MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NM2_PSEPK | 4307 | Outer membrane protein H1 OS=Pseudomonas putida (strain KT2440) GN=oprH PE=4 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 2609 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 2412 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=1 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPK | 2344 | ATP synthase subunit beta OS=Pseudomonas putida (strain KT2440) GN=atpD PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 3.1 | ATPB_PSEPK |
2344 | 49385 | 58 (49) | 20 (17) | 2.40 | |
| ATP synthase subunit beta OS=Pseudomonas putida (strain KT2440) GN=atpD PE=3 SV=1 | |||||||
58 peptide matches (33 non-duplicate, 25 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 278 |
1 |
586.3677 | 585.3604 | 585.3598 | 1.01 | 0 | 25 | 0.027 | 2Score > 22 indicates identity |
U | K.QLVAR.A |
| 286 |
587.3518 | 586.3445 | 586.3438 | 1.16 | 0 | 7 | 1.1 | 10Score > 25 indicates identity Score > 20 indicates homology |
U | K.QLVAR.A + Deamidated (NQ) | |
| 312 |
305.1848 | 608.3550 | 608.3533 | 2.79 | 0 | 16 | 0.028 | 1Score > 13 indicates identity |
U | K.YVSLK.D | |
| 501 |
715.4481 | 714.4408 | 714.4388 | 2.82 | 0 | 15 | 0.27 | 4Score > 22 indicates identity |
U | E.VSALLGR.M | |
| 502 |
358.2283 | 714.4420 | 714.4388 | 4.53 | 0 | 9 | 0.74 | 7Score > 20 indicates identity |
U | E.VSALLGR.M | |
| 658 |
790.3958 | 789.3885 | 789.3868 | 2.13 | 0 | 50 | 9.6e-005 | 1Score > 22 indicates identity |
U | K.DSNVLDK.V | |
| 659 |
395.7019 | 789.3892 | 789.3868 | 3.05 | 0 | 28 | 0.015 | 1Score > 22 indicates identity |
U | K.DSNVLDK.V | |
| 1075 |
2 |
481.2794 | 960.5442 | 960.5393 | 5.16 | 0 | 65 | 8.9e-007 | 1Score > 17 indicates identity |
U | K.VGLFGGAGVGK.T |
| 1076 |
961.5524 | 960.5451 | 960.5393 | 6.07 | 0 | 69 | 3.7e-007 | 1Score > 17 indicates identity |
U | K.VGLFGGAGVGK.T | |
| 1079 |
962.5441 | 961.5368 | 961.5345 | 2.40 | 0 | 47 | 8e-005 | 2Score > 18 indicates identity |
U | R.GVQYVLQR.Y | |
| 1081 |
1 |
481.7763 | 961.5380 | 961.5345 | 3.67 | 0 | 34 | 0.0016 | 1Score > 18 indicates identity |
U | R.GVQYVLQR.Y |
| 1082 |
482.2677 | 962.5208 | 962.5185 | 2.41 | 0 | 7 | 1.1 | 3Score > 20 indicates identity |
U | R.GVQYVLQR.Y + Deamidated (NQ) | |
| 1361 |
553.7922 | 1105.5698 | 1105.5624 | 6.77 | 1 | 12 | 0.083 | 1Score > 21 indicates identity Score > 13 indicates homology |
U | K.TVNMMELIR.N | |
| 1368 |
554.2952 | 1106.5758 | 1106.5641 | 10.6 | 1 | 9 | 0.83 | 2Score > 21 indicates identity |
U | R.TIAMGSTEGLK.R | |
| 1401 |
1121.6271 | 1120.6198 | 1120.6128 | 6.25 | 0 | 109 | 5.1e-011 | 1Score > 18 indicates identity |
U | K.DTIAGFSGILK.G | |
| 1403 |
1 |
561.3215 | 1120.6284 | 1120.6128 | 13.9 | 0 | 30 | 0.0034 | 1Score > 17 indicates identity |
U | K.DTIAGFSGILK.G |
| 1432 |
1 |
567.3181 | 1132.6216 | 1132.6162 | 4.83 | 1 | 63 | 1.8e-006 | 1Score > 20 indicates identity Score > 18 indicates homology |
U | R.VALTGLTMAEK.F |
| 1603 |
2 |
602.8379 | 1203.6612 | 1203.6499 | 9.40 | 0 | 40 | 0.00041 | 1Score > 19 indicates identity |
U | R.DVVPSVYNALK.V |
| 2077 |
484.2708 | 1449.7906 | 1449.7827 | 5.41 | 1 | 61 | 2.4e-006 | 1Score > 17 indicates identity |
U | R.YTLAGTEVSALLGR.M | |
| 2079 |
2 |
725.9044 | 1449.7942 | 1449.7827 | 7.94 | 1 | 98 | 3.8e-010 | 1Score > 17 indicates identity |
U | R.YTLAGTEVSALLGR.M |
| 2391 |
533.3019 | 1596.8839 | 1596.8723 | 7.24 | 0 | 86 | 9.2e-009 | 1Score > 18 indicates identity |
U | R.GLDVVDTGAAISVPVGK.A | |
| 2392 |
1 |
799.4497 | 1596.8848 | 1596.8723 | 7.85 | 0 | 102 | 2.2e-010 | 1Score > 18 indicates identity |
U | R.GLDVVDTGAAISVPVGK.A |
| 2502 |
823.4151 | 1644.8156 | 1644.7930 | 13.8 | 0 | 73 | 3.4e-007 | 1Score > 21 indicates identity |
U | K.VALVYGQMNEPPGNR.L + Deamidated (NQ) | |
| 2559 |
6 |
834.9926 | 1667.9706 | 1667.9610 | 5.76 | 1 | 116 | 2.8e-012 | 1Score > 13 indicates identity |
U | R.IVQIIGAVIDVEFPR.D |
| 2561 |
1 |
556.9987 | 1667.9743 | 1667.9610 | 7.93 | 1 | 75 | 3.5e-008 | 1Score > 13 indicates identity |
U | R.IVQIIGAVIDVEFPR.D |
| 2571 |
557.3318 | 1668.9736 | 1668.9451 | 17.1 | 1 | 76 | 2.3e-008 | 1Score > 13 indicates identity |
U | R.IVQIIGAVIDVEFPR.D + Deamidated (NQ) | |
| 2655 |
2 |
571.3292 | 1710.9658 | 1710.9556 | 5.94 | 1 | 53 | 1.1e-005 | 1Score > 16 indicates identity |
U | K.YVSLKDTIAGFSGILK.G |
| 3087 |
979.5160 | 1957.0174 | 1956.9986 | 9.64 | 1 | 82 | 3.1e-008 | 1Score > 19 indicates identity |
U | R.FLSQPFFVAEVFTGSPGK.Y | |
| 3089 |
2 |
653.6786 | 1958.0140 | 1957.9826 | 16.0 | 1 | 77 | 8.3e-008 | 1Score > 19 indicates identity |
U | R.FLSQPFFVAEVFTGSPGK.Y + Deamidated (NQ) |
| 3091 |
653.6801 | 1958.0185 | 1957.9826 | 18.3 | 1 | (77) | 7.8e-008 | 1Score > 19 indicates identity |
U | R.FLSQPFFVAEVFTGSPGK.Y + Deamidated (NQ) | |
| 3092 |
653.6810 | 1958.0212 | 1957.9826 | 19.7 | 1 | (61) | 3.4e-006 | 1Score > 19 indicates identity |
U | R.FLSQPFFVAEVFTGSPGK.Y + Deamidated (NQ) | |
| 3090 |
1 |
980.0152 | 1958.0158 | 1957.9826 | 17.0 | 1 | 82 | 2.9e-008 | 1Score > 19 indicates identity |
U | R.FLSQPFFVAEVFTGSPGK.Y + Deamidated (NQ) |
| 3132 |
995.5179 | 1989.0212 | 1989.0055 | 7.93 | 0 | 131 | 4.6e-013 | 1Score > 20 indicates identity |
U | R.DIASLGIYPAVDPLDSTSR.Q | |
| 3857 |
2 |
813.0813 | 2436.2221 | 2436.1921 | 12.3 | 1 | 71 | 3.3e-007 | 1Score > 19 indicates identity |
U | R.GIHQPAPSFADQAGGNDLLETGIK.V + Deamidated (NQ) |
| 4710 |
961.9933 | 3843.9441 | 3843.9055 | 10.0 | 1 | 26 | 0.0053 | 1Score > 15 indicates identity |
U | K.EGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSR.D + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| Q88EE9_PSEPK |
25 | 0 | 3.1 | ||
| description | |||||
| 1 sameset of Q88EE9_PSEPK | |||||
| Q88KZ9_PSEPK |
25 | 98034 | |||
| DNA topoisomerase 1 OS=Pseudomonas putida (strain KT2440) GN=topA PE=3 SV=1 | |||||
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88ES5_PSEPK | 2322 | Flagellin OS=Pseudomonas putida (strain KT2440) GN=fliC PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88L46_PSEPK | 1745 | Outer membrane protein OprF OS=Pseudomonas putida (strain KT2440) GN=oprF PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 1711 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FB0_PSEPK | 1295 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=kgdB PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FA7_PSEPK | 1233 | Succinate dehydrogenase flavoprotein subunit OS=Pseudomonas putida (strain KT2440) GN=sdhA PE=3 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPK | 843 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FA8_PSEPK | 775 | Succinate dehydrogenase, iron-sulfur protein OS=Pseudomonas putida (strain KT2440) GN=sdhB PE=4 SV=1 |
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