MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NM2_PSEPK | 4307 | Outer membrane protein H1 OS=Pseudomonas putida (strain KT2440) GN=oprH PE=4 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 2609 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 2412 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=1 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPK | 2344 | ATP synthase subunit beta OS=Pseudomonas putida (strain KT2440) GN=atpD PE=3 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88ES5_PSEPK | 2322 | Flagellin OS=Pseudomonas putida (strain KT2440) GN=fliC PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88L46_PSEPK | 1745 | Outer membrane protein OprF OS=Pseudomonas putida (strain KT2440) GN=oprF PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 1711 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FB0_PSEPK | 1295 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=kgdB PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FA7_PSEPK | 1233 | Succinate dehydrogenase flavoprotein subunit OS=Pseudomonas putida (strain KT2440) GN=sdhA PE=3 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPK | 843 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FA8_PSEPK | 775 | Succinate dehydrogenase, iron-sulfur protein OS=Pseudomonas putida (strain KT2440) GN=sdhB PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 10.1 | Q88FA8_PSEPK |
775 | 26610 | 29 (26) | 13 (12) | 3.50 | |
| Succinate dehydrogenase, iron-sulfur protein OS=Pseudomonas putida (strain KT2440) GN=sdhB PE=4 SV=1 | |||||||
29 peptide matches (20 non-duplicate, 9 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 344 |
629.3636 | 628.3563 | 628.3544 | 3.04 | 0 | 37 | 0.00077 | 1Score > 18 indicates identity |
U | K.GLNPTK.A | |
| 345 |
315.1856 | 628.3566 | 628.3544 | 3.55 | 0 | 18 | 0.054 | 1Score > 18 indicates identity |
U | K.GLNPTK.A | |
| 378 |
652.3913 | 651.3840 | 651.3816 | 3.66 | 0 | 11 | 0.21 | 3Score > 16 indicates identity |
U | K.AIGHVR.N | |
| 379 |
326.6993 | 651.3840 | 651.3816 | 3.70 | 0 | 27 | 0.0042 | 1Score > 16 indicates identity |
U | K.AIGHVR.N | |
| 390 |
665.3636 | 664.3563 | 664.3544 | 2.87 | 1 | 28 | 0.0097 | 1Score > 20 indicates identity |
U | K.VEVYR.Y | |
| 391 |
333.1855 | 664.3564 | 664.3544 | 3.06 | 1 | 23 | 0.031 | 1Score > 20 indicates identity |
U | K.VEVYR.Y | |
| 491 |
354.6884 | 707.3622 | 707.3602 | 2.85 | 0 | 19 | 0.056 | 1Score > 22 indicates identity Score > 19 indicates homology |
U | R.FLADSR.D | |
| 772 |
422.7131 | 843.4116 | 843.4086 | 3.58 | 1 | 45 | 0.00015 | 1Score > 19 indicates identity |
U | R.LQSPEDR.D | |
| 1367 |
554.2518 | 1106.4890 | 1106.4880 | 0.94 | 0 | 44 | 0.00018 | 1Score > 19 indicates identity |
U | R.YNPDTDSAPK.M | |
| 1820 |
1 |
660.3819 | 1318.7492 | 1318.7397 | 7.22 | 0 | 80 | 2.5e-008 | 1Score > 16 indicates identity |
U | K.FLGPAALLQAYR.F |
| 1839 |
3 |
665.3462 | 1328.6778 | 1328.6686 | 6.94 | 0 | 56 | 1.6e-005 | 1Score > 21 indicates identity |
U | R.DLVVDMSIFYK.Q |
| 1855 |
1 |
671.9117 | 1341.8088 | 1341.7941 | 11.0 | 0 | 54 | 4.2e-006 | 1Score > 13 indicates identity |
U | K.DLMVLDVLALIK.E |
| 1885 |
679.9121 | 1357.8096 | 1357.7891 | 15.2 | 0 | 60 | 9.1e-007 | 1Score > 13 indicates identity |
U | K.DLMVLDVLALIK.E + Oxidation (M) | |
| 1902 |
2 |
683.8569 | 1365.6992 | 1365.6929 | 4.67 | 0 | 50 | 4.6e-005 | 1Score > 19 indicates identity |
U | R.LASLDDPFSVFR.C |
| 2063 |
721.9120 | 1441.8094 | 1441.7963 | 9.13 | 0 | 44 | 0.00013 | 1Score > 18 indicates identity |
U | K.NGLACITPLSAVVK.G | |
| 2065 |
1 |
481.6598 | 1441.9576 | 1441.9497 | 5.48 | 0 | 47 | 2.1e-005 | 1Score > 13 indicates identity |
U | K.LVLRPLPGLPVIR.D |
| 2068 |
1 |
721.9896 | 1441.9646 | 1441.9497 | 10.4 | 0 | 39 | 0.00013 | 1Score > 13 indicates identity |
U | K.LVLRPLPGLPVIR.D |
| 2067 |
721.9894 | 1441.9642 | 1441.9497 | 10.1 | 0 | (9) | 0.12 | 1Score > 13 indicates identity |
U | K.LVLRPLPGLPVIR.D | |
| 2826 |
599.3305 | 1794.9697 | 1794.9628 | 3.82 | 1 | 49 | 4.4e-005 | 1Score > 18 indicates identity |
U | K.VKPFLQNDTPAPAIER.L | |
| 2827 |
898.9930 | 1795.9714 | 1795.9468 | 13.7 | 1 | 70 | 3.6e-007 | 1Score > 18 indicates identity |
U | K.VKPFLQNDTPAPAIER.L + Deamidated (NQ) | |
| 2828 |
599.6656 | 1795.9750 | 1795.9468 | 15.7 | 1 | 56 | 7.6e-006 | 1Score > 18 indicates identity |
U | K.VKPFLQNDTPAPAIER.L + Deamidated (NQ) |
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