MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:29:11 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4753)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
212 333.2071 332.1998
213 333.2073 332.2000
214 333.2073 332.2000
215 333.2075 332.2002
216 333.2076 332.2003
217 333.2077 332.2004
218 333.2077 332.2004
219 333.2248 332.2175
220 333.2270 332.2197
221 333.2273 332.2200
222 333.2274 332.2201
223 333.2277 332.2204
224 335.1865 334.1792
225 335.1870 334.1797
226 335.1875 334.1802
227 335.2212 334.2139
228 335.2223 334.2150
229 335.2224 334.2151
230 335.2225 334.2152
231 335.2225 334.2152
232 335.2229 334.2156
233 335.2230 334.2157
234 335.2232 334.2159
235 335.2232 334.2159
236 335.2234 334.2161
237 335.2234 334.2161
238 335.2403 334.2330
239 335.2430 334.2357
240 335.2431 334.2358
241 335.2432 334.2359
242 335.2434 334.2361
243 337.1835 336.1762
244 337.1837 336.1764
245 337.1838 336.1765
246 337.1840 336.1767
247 337.1841 336.1768
248 337.1844 336.1771
249 337.1863 336.1790
250 337.1866 336.1793
251 337.1866 336.1793
252 337.2015 336.1942
253 337.2015 336.1942
254 337.2018 336.1945
255 337.2020 336.1947
256 337.2024 336.1951
257 337.2032 336.1959
258 337.2039 336.1966
259 337.2570 336.2497
260 337.2575 336.2502
261 338.2401 337.2328
262 338.2413 337.2340
263 338.2414 337.2341
264 338.2415 337.2342
265 338.2416 337.2343
266 339.1635 338.1562
267 339.1637 338.1564
268 339.1654 338.1581
269 339.1995 338.1922
270 339.2034 338.1961
271 339.2737 338.2664
272 341.1476 340.1403
273 341.1771 340.1698
274 341.1796 340.1723
275 341.1814 340.1741
276 341.1968 340.1895
277 341.1969 340.1896
278 341.1969 340.1896
279 341.1972 340.1899
280 341.1975 340.1902
281 341.1978 340.1905
282 341.1994 340.1921
283 341.2166 340.2093
284 341.2173 340.2100
285 341.2173 340.2100
286 343.1937 342.1864
287 343.2319 342.2246
288 343.2971 342.2898
289 345.1450 344.1377
290 346.2605 345.2532
291 347.1853 346.1780
292 347.1860 346.1787
293 347.1862 346.1789
294 347.1863 346.1790
295 347.1864 346.1791
296 347.1865 346.1792
297 347.1870 346.1797
298 347.1873 346.1800
299 347.1874 346.1801
300 347.1889 346.1816
301 347.2054 346.1981
302 349.2018 348.1945
303 349.2020 348.1947
304 349.2021 348.1948
305 349.2022 348.1949
306 349.2025 348.1952
307 349.2027 348.1954
308 349.2028 348.1955
309 349.2029 348.1956
310 349.2029 348.1956
311 349.2029 348.1956
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