MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:29:11 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4753)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1070 372.6872 743.3598 743.3490 14.6 0 1 1.9 +1Score > 16 indicates identity EGTYFK
1892 511.2722 1020.5298 1020.5240 5.73 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
ETALDFIGR
2751 633.8244 1265.6342 1265.6438 -7.55 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
DIFSMILQQR + Oxidation (M)
2867 693.3414 1384.6682 1384.6946 -19.0 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
NANILRQDTPNK + 2 Deamidated (NQ)
4730 1100.7581 4399.0033 4399.0511 -10.9 1 1 2.6 +1Score > 17 indicates identity WMAIAKASIDDGTMVSGDLDLGYEGILGIWNGLEGLSNQNR + 4 Deamidated (NQ); Oxidation (M)
2698 611.8311 1221.6476 1221.6353 10.1 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
EPGNGHLEKIK + Deamidated (NQ)
2738 629.8005 1257.5864 1257.5990 -9.94 0 1 3.2 +1Score > 18 indicates identity YSSTFLSSNPR
4673 1163.5531 3487.6375 3487.5765 17.5 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
RFQQDDAHIFCTQDQIEQEIANIFEFLK + 5 Deamidated (NQ)
1293 860.4160 859.4087 859.3977 12.9 0 1 3.4 +1Score > 18 indicates identity WWSQPR + Deamidated (NQ)
2859 688.8450 1375.6754 1375.6765 -0.76 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
VAANMNRNINIK + 3 Deamidated (NQ); Oxidation (M)
3742 1021.9371 2041.8596 2041.8204 19.2 0 1 1.9 +1Score > 16 indicates identity SNGSSMASPNAELQQDDMK + Deamidated (NQ); 2 Oxidation (M)
3244 817.9081 1633.8016 1633.7907 6.69 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
RSSNVTNSNTIVSPR + 3 Deamidated (NQ)
3493 920.4440 1838.8734 1838.8898 -8.88 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NNSDYDQVNLLTTTIK + Deamidated (NQ)
3339 855.3578 1708.7010 1708.7289 -16.3 1 1 1.4 +1Score > 15 indicates identity GGNSNNGSSSTGKHFQK + 3 Deamidated (NQ)
3240 544.9421 1631.8045 1631.8042 0.16 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
KNAENPDLFLDINK + 2 Deamidated (NQ)
3645 987.0695 1972.1244 1972.1027 11.0 1 1 1 +1Score > 13 indicates identity VQLLNIVRNIATMTAGLK + 2 Deamidated (NQ); Oxidation (M)
3907 1061.4888 2120.9630 2120.9507 5.83 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
EEDQGFPVLMGGPLSNGMAR + Deamidated (NQ); Oxidation (M)
4088 1131.5520 2261.0894 2261.0782 4.99 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LLFMALNDEVFAIQMEAMK + 3 Oxidation (M)
931 333.2029 664.3912 664.3908 0.71 1 0 2.7 +1Score > 17 indicates identity KNIYK
2801 659.3288 1316.6430 1316.6394 2.73 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
SMPAQGVDDLIR + Oxidation (M)
866 624.3364 623.3291 623.3391 -16.0 0 0 3.4 +1Score > 18 indicates identity ATAPHK
4202 789.7214 2366.1424 2366.0995 18.1 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
SMLLSGTDNHLAGLGQMAEYAR + 2 Oxidation (M)
3061 760.3810 1518.7474 1518.7613 -9.12 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
MSQKVGHTGLAFAR + Deamidated (NQ); Oxidation (M)
1395 898.3911 897.3838 897.4014 -19.6 0 0 1.7 +1Score > 15 indicates identity GNASFVMR + Deamidated (NQ); Oxidation (M)
3724 1014.0004 2025.9862 2025.9643 10.8 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
DISINSWDLVNESYSKR + Deamidated (NQ)
3205 805.8698 1609.7250 1609.7233 1.08 1 0 4.5 +1Score > 19 indicates identity FSNSFTTQNNHRR + 2 Deamidated (NQ)
1054 371.1802 740.3458 740.3453 0.73 0 0 1.9 +1Score > 16 indicates identity GIDHNGK + Deamidated (NQ)
1122 391.1906 780.3666 780.3766 -12.8 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
YGSDALR
1261 425.1967 848.3788 848.3875 -10.2 0 0 3.7 +1Score > 19 indicates identity STNQQLR + 3 Deamidated (NQ)
2412 571.7616 1141.5086 1141.5186 -8.72 0 0 3 +1Score > 18 indicates identity HNVDNAMTPK + Oxidation (M)
1198 818.4114 817.4041 817.3930 13.6 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
LSNDVNR + Deamidated (NQ)
3562 952.9564 1903.8982 1903.8945 1.96 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
GEEPSADMVERAESLIR + Oxidation (M)
3327 568.6175 1702.8307 1702.8560 -14.9 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NDIQMDLGVVNLSLR + Deamidated (NQ); Oxidation (M)
2001 1044.5116 1043.5043 1043.5247 -19.5 0 0 4.3 +1Score > 19 indicates identity LQQEQLQR + 2 Deamidated (NQ)
2666 601.2970 1200.5794 1200.6026 -19.3 0 0 4.2 +1Score > 19 indicates identity WLLDNSPEVK + Deamidated (NQ)
3057 759.8602 1517.7058 1517.7362 -20.0 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
FNAVLENTPSSDPK
4116 1147.5597 2293.1048 2293.0631 18.2 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
DMLQSASGNISVNDDIDEILK + Deamidated (NQ); Oxidation (M)
4163 775.3820 2323.1242 2323.1583 -14.7 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
KFVEEENAATLQLGEEFQLK + Deamidated (NQ)
1662 968.4698 967.4625 967.4467 16.4 0 0 4.6 +1Score > 19 indicates identity GGATMMLGSK + Oxidation (M)
853 615.3472 614.3399 614.3387 1.92 0 0 2.5 +1Score > 17 indicates identity AKPNGK + Deamidated (NQ)
2858 688.8447 1375.6748 1375.6765 -1.20 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
VAANMNRNINIK + 3 Deamidated (NQ); Oxidation (M)
4004 1091.9956 2181.9766 2181.9370 18.2 0 0 4.5 +1Score > 19 indicates identity QTSSSSPATTGVGGSNGNSNSAGR + 2 Deamidated (NQ)
4660 863.4641 3449.8273 3449.8268 0.14 1 0 2.2 +1Score > 16 indicates identity LADMPSNEIATIFHANVLSTIKLIQAFHPLK + 2 Deamidated (NQ); Oxidation (M)
2722 623.3361 1244.6576 1244.6360 17.4 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
ERIATQDGNLK + Deamidated (NQ)
1753 991.4918 990.4845 990.4771 7.53 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
ATRVFPGDE
1791 500.2411 998.4676 998.4531 14.5 0 0 3.6 +1Score > 18 indicates identity WGQIGYMK + Deamidated (NQ); Oxidation (M)
2333 560.7728 1119.5310 1119.5230 7.18 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
SQVLECQQK + Deamidated (NQ)
3882 1055.5112 2109.0078 2109.0201 -5.79 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
TQVLKFNEYVHMTNNLR + 3 Deamidated (NQ)
4058 746.6814 2237.0224 2237.0416 -8.59 1 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
ISDNGGNLSMGQRQLLCLSR + 3 Deamidated (NQ); Oxidation (M)
3869 701.9990 2102.9752 2102.9942 -9.07 1 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
EKLEDQLQGMPFTAHVSR + 2 Deamidated (NQ); Oxidation (M)
4406 698.1235 2788.4649 2788.4224 15.2 1 0 3.4 +1Score > 18 indicates identity NISAHTSEVNFFNIAYLFIKNFAK + Deamidated (NQ)
894 641.3375 640.3302 640.3254 7.52 0 0 3.7 +1Score > 18 indicates identity YIAMK + Oxidation (M)
2525 582.8090 1163.6034 1163.6121 -7.43 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
AGWRLSSMLK + Oxidation (M)
3317 847.3821 1692.7496 1692.7698 -11.9 0 0 3.7 +1Score > 18 indicates identity LPIGDSSMEMIEEAR + Oxidation (M)
4026 734.7404 2201.1994 2201.2209 -9.76 1 0 2.9 +1Score > 17 indicates identity LATVTRVPVNPFDVSAIVFR + Deamidated (NQ)
2843 452.2327 1353.6763 1353.6928 -12.2 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
DKFLNIAQDYK
1376 891.4309 890.4236 890.4345 -12.2 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
TVNLNSNK + 2 Deamidated (NQ)
2281 553.2939 1104.5732 1104.5710 2.06 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
RQSMVEVTR
2711 618.8025 1235.5904 1235.6146 -19.5 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
QNFKVDSEIR + Deamidated (NQ)
1470 460.2497 918.4848 918.4732 12.7 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
ADLMLDIL + Oxidation (M)
4740 1010.0935 5045.4311 5045.3998 6.21 1 0 2.8 +1Score > 17 indicates identity VSMANAGPNTNACQFFITTSNEPMPHLDGKHVVFGQVISGLEDLIR + 2 Deamidated (NQ); 2 Oxidation (M)
1769 995.4869 994.4796 994.4971 -17.6 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
DSIEFSGLK
2266 552.2559 1102.4972 1102.5077 -9.47 0 0 3.8 +1Score > 18 indicates identity CNPNTEVLR + Deamidated (NQ)
2464 576.7684 1151.5222 1151.5095 11.1 0 0 4.7 +1Score > 19 indicates identity SPSNDNLYDK
3198 802.4115 1602.8084 1602.7790 18.4 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
IALENYWSHRNAK + 2 Deamidated (NQ)
1059 742.4113 741.4040 741.4021 2.60 0 0 1.9 +1Score > 15 indicates identity VPSQSPK
1189 814.3699 813.3626 813.3657 -3.81 0 0 1.7 +1Score > 15 indicates identity TFDQFR + Deamidated (NQ)
1443 456.2314 910.4482 910.4409 8.03 0 0 4.2 +1Score > 19 indicates identity WLANHDR
2593 589.8148 1177.6150 1177.5979 14.6 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
NQIYDTSPLK
3380 873.9319 1745.8492 1745.8294 11.4 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
WNNQMKPLVQNLNK + 4 Deamidated (NQ); Oxidation (M)
1749 991.4674 990.4601 990.4692 -9.16 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
SLDMGPQVK + Deamidated (NQ); Oxidation (M)
2917 718.3453 1434.6760 1434.6561 13.9 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
QLHTMEQTQYR + Deamidated (NQ)
3096 513.5787 1537.7143 1537.6864 18.1 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
EMEAMRPEGTLSR + 2 Oxidation (M)
3423 889.4370 1776.8594 1776.8570 1.36 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
ELEDFAFPDPETIVR
859 309.6662 617.3178 617.3173 0.90 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
IGNWK + Deamidated (NQ)
1569 472.7390 943.4634 943.4610 2.56 0 0 3.8 +1Score > 18 indicates identity QSLAPNANK + 2 Deamidated (NQ)
1893 1021.5491 1020.5418 1020.5240 17.5 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
ETALDFIGR
4446 962.7916 2885.3530 2885.4083 -19.2 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
GYPVNQPNEQVGLPSDFAKPQSIIQR + 4 Deamidated (NQ)
2775 643.8293 1285.6440 1285.6375 5.13 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
EVGNVQNNEKR
3137 521.9329 1562.7769 1562.7953 -11.8 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
SRPYNYIPSGRPR + Deamidated (NQ)
3454 605.2972 1812.8698 1812.8981 -15.6 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LGPNYLHIPVNCPYR + Deamidated (NQ)
4155 1156.0460 2310.0774 2310.0585 8.21 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
SATRNSSFSISQQGQGQGQGGAR + 2 Deamidated (NQ)
4141 768.7222 2303.1448 2303.1546 -4.26 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
VARSLDNAASTWIWTVNELR + 2 Deamidated (NQ)
1549 939.4653 938.4580 938.4644 -6.76 1 0 4.9 +1Score > 20 indicates identity KVSGNFMR + Deamidated (NQ)
3081 767.3748 1532.7350 1532.7259 5.95 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
ARAWDEFVEANPK + Deamidated (NQ)
3994 725.3459 2173.0159 2172.9845 14.4 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
ENPDVVLVMDGGQGTELENR + 2 Deamidated (NQ)
1891 1021.5353 1020.5280 1020.5240 3.94 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
ETALDFIGR
2761 636.3211 1270.6276 1270.6292 -1.23 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
LLQDEQLPNAK + 3 Deamidated (NQ)
3595 968.4989 1934.9832 1934.9870 -1.95 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
IISNMNGSVVDSLELLSK + Deamidated (NQ); Oxidation (M)
1 300.2086 299.2013
2 301.1436 300.1363
3 301.1436 300.1363
4 301.1437 300.1364
5 301.1440 300.1367
6 301.1440 300.1367
7 301.1440 300.1367
8 301.1444 300.1371
9 301.2213 300.2140
10 303.8864 302.8791
11 303.8865 302.8792
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