MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:29:11 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 4753)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2012 1048.5187 1047.5114 1047.5025 8.50 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
FKYANDYK
4130 766.6998 2297.0776 2297.0369 17.7 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LSDELEDSVVYSNQNTPMQK + Deamidated (NQ)
1738 987.4643 986.4570 986.4743 -17.5 0 2 2.6 +1Score > 18 indicates identity NPLPSMSPK + Deamidated (NQ); Oxidation (M)
2847 682.3728 1362.7310 1362.7143 12.3 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
STPPSDPAKAAPPK
1540 469.7159 937.4172 937.3997 18.7 0 2 2.3 +1Score > 18 indicates identity AMGGTCVNK + Deamidated (NQ)
2074 1060.5176 1059.5103 1059.5057 4.36 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
NGSQQQNKR + Deamidated (NQ)
3374 871.9055 1741.7964 1741.8271 -17.6 0 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
NEIGGQNHVSFSVQPK + 2 Deamidated (NQ)
4269 827.4291 2479.2655 2479.2853 -8.00 1 2 3.5 +1Score > 20 indicates identity RTLASGIGTSGSGLGGIIFNLGMQR + Deamidated (NQ); Oxidation (M)
897 321.1849 640.3552 640.3656 -16.2 0 1 1.1 +1Score > 14 indicates identity VAAQPR
4756 1118.5378 5587.6526 5587.6623 -1.74 0 1 1.7 +1Score > 16 indicates identity HFVPVGEYQGQLFGDSSVSPLANYVSQEEWASIISDINGLLAAAYNPVQFR + 5 Deamidated (NQ)
4241 813.7852 2438.3338 2438.3090 10.2 1 1 1.6 +1Score > 16 indicates identity LESLGVIPDTMATLAPKAEVNLR + Deamidated (NQ)
1216 830.3930 829.3857 829.3930 -8.72 0 1 1.5 +1Score > 16 indicates identity SLNQPNR + 2 Deamidated (NQ)
4089 754.7054 2261.0944 2261.0782 7.17 0 1 0.77 +1Score > 21 indicates identity
Score > 13 indicates homology
LLFMALNDEVFAIQMEAMK + 3 Oxidation (M)
2674 604.3069 1206.5992 1206.5914 6.49 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
MDNLKLQDSK + Oxidation (M)
3054 506.2484 1515.7234 1515.7165 4.52 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
NQQQSTGTGNGVVPK + 2 Deamidated (NQ)
2885 701.3397 1400.6648 1400.6684 -2.57 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
FGAPQDLQPGQSR + Deamidated (NQ)
2776 644.2968 1286.5790 1286.5747 3.37 1 1 2.7 +1Score > 18 indicates identity YLECSAKMNR + Oxidation (M)
1665 968.4799 967.4726 967.4909 -18.9 1 1 3.4 +1Score > 19 indicates identity MVYAANKR + Oxidation (M)
4587 1064.8428 3191.5066 3191.5544 -15.0 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
HVTTLISMDDLSENELKSINDNFLTNVK + 2 Deamidated (NQ)
3366 867.3951 1732.7756 1732.8090 -19.3 1 1 3 +1Score > 19 indicates identity WCRLASELTDPEEK
2942 723.8749 1445.7352 1445.7548 -13.5 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LATSTMNNLIPQK + Oxidation (M)
4641 1140.5413 3418.6021 3418.6498 -14.0 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
QNLEHTILMLKAMGINDLLNFEFMDPPPK + 2 Deamidated (NQ); 3 Oxidation (M)
1060 371.7096 741.4046 741.3956 12.2 1 1 1.4 +1Score > 15 indicates identity KHICGK
2414 571.7631 1141.5116 1141.5186 -6.09 0 1 2.4 +1Score > 18 indicates identity HNVDNAMTPK + Oxidation (M)
1058 742.4113 741.4040 741.4021 2.60 0 1 1.4 +1Score > 15 indicates identity VPSQSPK
1252 844.4264 843.4191 843.4338 -17.4 0 1 3.2 +1Score > 19 indicates identity NDSGLPLK + Deamidated (NQ)
3759 1029.4778 2056.9410 2056.9346 3.13 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
DNAANMKDYLITMWESR
828 586.2842 585.2769 585.2870 -17.3 0 1 1.2 +1Score > 15 indicates identity ANPQR + Deamidated (NQ)
2170 541.2631 1080.5116 1080.5200 -7.69 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
ANNPQHGITK + 2 Deamidated (NQ)
1342 440.2571 878.4996 878.5086 -10.2 0 1 2.4 +1Score > 18 indicates identity GAHVAVGLR
2712 618.8149 1235.6152 1235.5994 12.9 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
ALKTEGGSGDSSK
1283 857.4203 856.4130 856.4290 -18.7 0 1 3.1 +1Score > 19 indicates identity QDPAGEIK
3578 960.4537 1918.8928 1918.8917 0.62 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
GLHLNEDIYAGMNAMLR + 2 Deamidated (NQ)
1170 800.4280 799.4207 799.4300 -11.6 0 1 2.7 +1Score > 18 indicates identity NAQQAIR
2952 724.3734 1446.7322 1446.7388 -4.53 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
LATSTMNNLIPQK + Deamidated (NQ); Oxidation (M)
2967 487.2389 1458.6949 1458.6660 19.8 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
ALNMTSSALSEYR + Deamidated (NQ); Oxidation (M)
2524 582.8063 1163.5980 1163.5791 16.3 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
RCQAIGLMSK + Deamidated (NQ)
3632 656.0320 1965.0742 1965.0571 8.70 1 1 1.8 +1Score > 16 indicates identity AWDAIAKQLAPSNPLELK + Deamidated (NQ)
3672 664.6440 1990.9102 1990.9484 -19.2 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
TLPDNDANATIVDDFVSGK
2644 1190.5708 1189.5635 1189.5575 5.09 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
SATQQQQQLR + 3 Deamidated (NQ)
1783 997.4606 996.4533 996.4400 13.4 0 1 3.4 +1Score > 19 indicates identity SEGDSFIDK
4280 830.4541 2488.3405 2488.2957 18.0 0 1 2.4 +1Score > 17 indicates identity MLISLETIHQNISEMYPVLLK + Deamidated (NQ); Oxidation (M)
1459 458.2324 914.4502 914.4392 12.1 0 1 2.6 +1Score > 18 indicates identity QSLQMHR + Oxidation (M)
2804 660.3364 1318.6582 1318.6802 -16.7 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
AMILPTSKEEGK + Oxidation (M)
3282 834.3949 1666.7752 1666.7910 -9.47 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LQDLNKEIHNDGNR + 2 Deamidated (NQ)
2854 685.3195 1368.6244 1368.5979 19.4 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
KGNEICNNYGAK + 2 Deamidated (NQ)
3104 773.8999 1545.7852 1545.7861 -0.54 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
VLMNIFSNLEHTK + Deamidated (NQ)
4536 756.8776 3023.4813 3023.4876 -2.07 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
VNQNLLESHSFINYKQNVEYVNIVR + 4 Deamidated (NQ)
1881 1019.4826 1018.4753 1018.4679 7.26 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
DETRNQQK + Deamidated (NQ)
3131 781.8963 1561.7780 1561.7624 10.0 0 1 0.95 +1Score > 22 indicates identity
Score > 13 indicates homology
AELLSFTNNIPEGR + 2 Deamidated (NQ)
4539 1010.4532 3028.3378 3028.3897 -17.2 0 1 3.5 +1Score > 19 indicates identity ALQDSSSAHATNNSYLSQGSSLTIQFGNK + 3 Deamidated (NQ)
3630 983.4482 1964.8818 1964.8745 3.73 1 1 3.7 +1Score > 19 indicates identity MSNNTETDKQVNIGPSGR + 2 Deamidated (NQ); Oxidation (M)
3765 1030.5248 2059.0350 2059.0731 -18.5 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
MSSVAAAQNKINDLLEAIR + Oxidation (M)
2118 1069.5135 1068.5062 1068.5161 -9.25 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
EEAMYILGK + Oxidation (M)
1168 400.7174 799.4202 799.4188 1.80 1 1 2.8 +1Score > 18 indicates identity LGDPDRK
1378 446.2252 890.4358 890.4531 -19.4 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
GLMISGGEK
3392 585.2657 1752.7753 1752.8029 -15.7 1 1 3.8 +1Score > 19 indicates identity DLDGNKLCAFYNPPK + 2 Deamidated (NQ)
3246 818.3850 1634.7554 1634.7285 16.5 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
SANPVPPTNNTNTNGH + Deamidated (NQ)
2260 551.2820 1100.5494 1100.5713 -19.9 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
TINLNPKGDK + 2 Deamidated (NQ)
4705 916.9564 3663.7965 3663.8573 -16.6 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
GGALLNATNAALHVVENQYLFHSVKDVTTLFYR + 3 Deamidated (NQ)
3093 768.8679 1535.7212 1535.7467 -16.6 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
AVGDSENIFSAADIK
1733 986.4759 985.4686 985.4828 -14.4 0 1 2.8 +1Score > 18 indicates identity AEPLATNNR + Deamidated (NQ)
4385 689.8419 2755.3385 2755.3044 12.4 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
AMTFSIECELSQNGNIRLLNDSLK + 3 Deamidated (NQ)
2768 639.3297 1276.6448 1276.6333 9.06 0 1 0.96 +1Score > 22 indicates identity
Score > 13 indicates homology
GDILISGNSICK + Deamidated (NQ)
1258 848.4064 847.3991 847.3923 8.08 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
SLNEANAK + 2 Deamidated (NQ)
3069 509.2592 1524.7558 1524.7613 -3.62 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
FFDEAPVLFVEGR
3794 1037.5032 2072.9918 2072.9803 5.56 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LPQGTNVSDYTIPHYDPR + Deamidated (NQ)
3887 705.3387 2112.9943 2112.9885 2.74 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NLEPTIMSLFTGASNAESSK + Deamidated (NQ); Oxidation (M)
1501 464.7410 927.4674 927.4702 -2.93 0 1 2.7 +1Score > 18 indicates identity YETFLQK
3939 1069.0087 2136.0028 2135.9840 8.81 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
SNQSMIINRNNCVIWDR + Deamidated (NQ); Oxidation (M)
4285 834.0410 2499.1012 2499.1322 -12.4 1 1 3.4 +1Score > 19 indicates identity KNSEVESPSLINTSDMAFEQGSK + 2 Deamidated (NQ)
4372 912.4583 2734.3531 2734.3128 14.7 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
QIVARNSQLTLCGMMVQYFNSLK + 2 Deamidated (NQ); 2 Oxidation (M)
1758 496.7443 991.4740 991.4644 9.71 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
NNLMIADGK + Deamidated (NQ); Oxidation (M)
1118 778.3750 777.3677 777.3657 2.59 0 1 0.98 +1Score > 20 indicates identity
Score > 13 indicates homology
NGSPFQK + Deamidated (NQ)
4733 1127.3445 4505.3489 4505.3560 -1.58 1 1 0.85 +1Score > 13 indicates identity AFAYMYMAIVLTSLLVLNYSLAMVMGVLAFPMTRTTTILK + 3 Oxidation (M)
3077 765.9110 1529.8074 1529.8300 -14.8 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NLLDNTTLQIEKK + Deamidated (NQ)
4674 1165.5756 3493.7050 3493.7733 -19.6 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
KVFFSVMLGLGLPLLFTMTLASAAMACTQTNK + Deamidated (NQ); 2 Oxidation (M)
3140 782.8708 1563.7270 1563.7416 -9.32 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
QEIQRGSPYEDIK + 2 Deamidated (NQ)
3161 792.8664 1583.7182 1583.7476 -18.5 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
YALIGDCTMHFLK + Oxidation (M)
1817 336.1556 1005.4450 1005.4259 19.0 0 1 2.3 +1Score > 17 indicates identity MHLMQNSK + 2 Deamidated (NQ); Oxidation (M)
4658 861.4396 3441.7293 3441.7378 -2.46 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
NEALFTTGVMDIKPYSINIIPGEVSFSLDVR + Deamidated (NQ); Oxidation (M)
3323 849.4070 1696.7994 1696.8090 -5.62 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NAVEQILLEQHNCK + 2 Deamidated (NQ)
3949 715.3611 2143.0615 2143.0545 3.24 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
QVISQSPEGAALQKEWTNR + 2 Deamidated (NQ)
4391 922.4780 2764.4122 2764.3959 5.88 1 1 4.1 +1Score > 19 indicates identity QQAAETKELQQIPPQLLFVHWQK + 5 Deamidated (NQ)
2141 1076.5073 1075.5000 1075.5033 -3.06 0 1 4.2 +1Score > 20 indicates identity EVSQDELGAK + Deamidated (NQ)
2857 687.3440 1372.6734 1372.6582 11.1 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
NIEASVQPSRDR + 2 Deamidated (NQ)
4675 875.6888 3498.7261 3498.7213 1.38 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
TATIIHLQNSIKELQSQLDVSSSQNDQLEEK + 3 Deamidated (NQ)
3206 806.3700 1610.7254 1610.7293 -2.41 1 1 4.1 +1Score > 19 indicates identity MECFARDNAVLNR + Oxidation (M)
1454 457.2286 912.4426 912.4263 18.0 0 1 3.7 +1Score > 19 indicates identity IFTEEMK + Oxidation (M)
4403 929.4742 2785.4008 2785.4061 -1.92 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
ILPYDSRDNEIPILEPPSYEPLSK + Deamidated (NQ)
2479 578.2640 1154.5134 1154.5203 -5.97 0 1 3.4 +1Score > 18 indicates identity NHLNNEGLDK + 2 Deamidated (NQ)
2731 625.3372 1248.6598 1248.6714 -9.23 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
NKIIESPSFSK
1138 393.1941 784.3736 784.3715 2.73 0 1 2.7 +1Score > 17 indicates identity TAAHQEK + Deamidated (NQ)
3631 655.9687 1964.8843 1964.9215 -18.9 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
SNVDEFDTALDSPSVQIK + Deamidated (NQ)
1478 461.7554 921.4962 921.4920 4.65 0 1 0.97 +1Score > 21 indicates identity
Score > 13 indicates homology
QNQLPPPK + Deamidated (NQ)
1629 480.7676 959.5206 959.5162 4.68 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
NHIRQHR
3038 755.3407 1508.6668 1508.6664 0.30 0 1 4.2 +1Score > 19 indicates identity MNSNSNLNGILEGK + 3 Deamidated (NQ); Oxidation (M)
3491 919.8867 1837.7588 1837.7709 -6.58 0 1 1.3 +1Score > 14 indicates identity MEDDLENAGNEAISAMK + Deamidated (NQ)
4726 1053.2776 4209.0813 4209.0003 19.2 1 1 3 +1Score > 18 indicates identity MASWDTISCVRLGSALACSQDEILPILLQFHQSISSC + Oxidation (M)
1732 493.7312 985.4478 985.4465 1.40 0 1 2.2 +1Score > 17 indicates identity HGQSEVNSK + Deamidated (NQ)
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