MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:29:11 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4753)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1021 361.7016 721.3886 721.3759 17.7 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
FTLDAR
914 328.1744 654.3342 654.3449 -16.3 0 3 1.3 +1Score > 17 indicates identity IGNSHK
2574 588.7809 1175.5472 1175.5611 -11.8 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
WAPVNSPNYK + Deamidated (NQ)
2807 662.8403 1323.6660 1323.6459 15.2 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
NFLVFQGDVER + Deamidated (NQ)
2693 610.3024 1218.5902 1218.6067 -13.5 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
MTPLKNNWAK + Deamidated (NQ); Oxidation (M)
1827 504.2498 1006.4850 1006.4832 1.87 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
NIQQYNAR + Deamidated (NQ)
1173 401.1969 800.3792 800.3664 16.0 0 3 1.2 +1Score > 16 indicates identity NAQEDPK
3138 782.4104 1562.8062 1562.8304 -15.4 1 3 0.66 +1Score > 22 indicates identity
Score > 14 indicates homology
LYQQSEQLAVVRK + 2 Deamidated (NQ)
857 618.2739 617.2666 617.2769 -16.6 0 3 0.74 +1Score > 14 indicates identity QEGER
1194 408.6964 815.3782 815.3773 1.12 0 3 1 +1Score > 16 indicates identity NNDPTVR + Deamidated (NQ)
1257 848.3794 847.3721 847.3858 -16.1 0 3 1.4 +1Score > 17 indicates identity MSEAAAPR + Oxidation (M)
4233 809.3663 2425.0771 2425.1141 -15.3 1 3 2.2 +1Score > 19 indicates identity KGELEEDCEMAGGIIIGQGYEK
1115 387.6912 773.3678 773.3668 1.41 0 3 1.6 +1Score > 17 indicates identity QLDNER
3514 927.4623 1852.9100 1852.9418 -17.1 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
LAGVPQELPQDVKDSQK + 2 Deamidated (NQ)
2045 1054.4805 1053.4732 1053.4615 11.2 0 3 1.8 +1Score > 18 indicates identity TDDSFSSAPK
1956 518.2064 1034.3982 1034.3941 4.02 0 3 0.52 +1Score > 13 indicates identity QDNNHYDK + 2 Deamidated (NQ)
3267 825.8732 1649.7318 1649.7099 13.3 0 3 2.6 +1Score > 20 indicates identity MSQMNTFTMSQALK + Deamidated (NQ); 2 Oxidation (M)
3763 687.0266 2058.0580 2058.0745 -8.05 1 3 0.83 +1Score > 21 indicates identity
Score > 14 indicates homology
IGDFGLAKNVQKPQESLGR + 2 Deamidated (NQ)
3767 1030.5289 2059.0432 2059.0731 -14.5 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
MSSVAAAQNKINDLLEAIR + Oxidation (M)
3438 899.9941 1797.9736 1797.9526 11.7 1 3 2.2 +1Score > 19 indicates identity FGSVLFGELRNAVFSR
3446 903.3924 1804.7702 1804.7533 9.37 0 3 1.2 +1Score > 16 indicates identity ASSQSSSPNTNPHSQMK + 2 Deamidated (NQ); Oxidation (M)
2865 692.8527 1383.6908 1383.6994 -6.15 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
KNNAAAASPDSLPK + Deamidated (NQ)
3596 968.5120 1935.0094 1935.0425 -17.1 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
QNIHPVITESAKTELVR + Deamidated (NQ)
4612 1097.8180 3290.4322 3290.4801 -14.6 1 3 1.5 +1Score > 17 indicates identity MVVMNPNNWHWVDKNTLPWTNDYLER + 3 Deamidated (NQ); Oxidation (M)
4450 967.4770 2899.4092 2899.4109 -0.60 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
QQINAMVLSHWLQDVCGFKPNAAEK + Oxidation (M)
923 329.1823 656.3500 656.3428 11.0 0 3 1.4 +1Score > 17 indicates identity MVVHR + Oxidation (M)
1598 475.7388 949.4630 949.4730 -10.4 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
HNHSTLNK
2579 588.8234 1175.6322 1175.6186 11.6 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
QLPNVLNYSK + Deamidated (NQ)
3286 834.9963 1667.9780 1667.9458 19.4 0 3 0.56 +1Score > 13 indicates identity SIRPLDVEAIINTVK + Deamidated (NQ)
4040 1108.5117 2215.0088 2215.0141 -2.38 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
GINGLQQQQQPSQQYPDQR + 3 Deamidated (NQ)
1256 846.4236 845.4163 845.4065 11.6 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
MSAPAPTR + Oxidation (M)
3132 521.6004 1561.7794 1561.7487 19.7 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
VYFADEPGMYKVK + Oxidation (M)
2766 638.8337 1275.6528 1275.6347 14.3 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
NVGLSETELWK + Deamidated (NQ)
2794 655.3245 1308.6344 1308.6098 18.8 0 3 0.77 +1Score > 21 indicates identity
Score > 14 indicates homology
ANIQNHYYTGK + Deamidated (NQ)
1673 485.7467 969.4788 969.4953 -17.0 0 2 1.7 +1Score > 17 indicates identity VTNPMPAPK + Oxidation (M)
2739 629.8022 1257.5898 1257.5990 -7.24 0 2 2.1 +1Score > 18 indicates identity YSSTFLSSNPR
2583 589.2988 1176.5830 1176.5696 11.4 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
DGINGVEMTLK + Deamidated (NQ)
3059 506.9099 1517.7079 1517.7362 -18.6 0 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
FNAVLENTPSSDPK
801 544.3099 543.3026 543.3129 -18.9 1 2 1.2 +1Score > 16 indicates identity RSPGK
1406 899.4943 898.4870 898.4985 -12.7 1 2 1.5 +1Score > 17 indicates identity DASVPRVR
4195 787.3704 2359.0894 2359.0862 1.33 0 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
MSHEGFQIPTNLDAAAAGAAQSR + Deamidated (NQ); Oxidation (M)
2703 613.2924 1224.5702 1224.5510 15.7 0 2 2.9 +1Score > 19 indicates identity ALQDAVNSYDK + 2 Deamidated (NQ)
3850 698.9726 2093.8960 2093.9350 -18.7 0 2 1.5 +1Score > 17 indicates identity DDIPEEIIQEMDDLYEK
2855 685.3445 1368.6744 1368.6521 16.3 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
ATKVSDVQYDSR + Deamidated (NQ)
975 342.1960 682.3774 682.3762 1.82 0 2 0.94 +1Score > 15 indicates identity IPSNPR
1655 484.2132 966.4118 966.4117 0.16 0 2 1.4 +1Score > 16 indicates identity CDVPFSDK
2680 604.8254 1207.6362 1207.6350 1.06 0 2 2.9 +1Score > 19 indicates identity SPLQGFGLFSR
3231 543.5922 1627.7548 1627.7729 -11.2 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
GQEAPNQPFTPAAGLK + 3 Deamidated (NQ)
3680 998.4747 1994.9348 1994.9181 8.41 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
RIQEQNNSVEIYSDGNK + 2 Deamidated (NQ)
1495 464.7099 927.4052 927.4120 -7.30 0 2 1.3 +1Score > 16 indicates identity YDMGTVSR
2480 578.2701 1154.5256 1154.5203 4.60 0 2 2.2 +1Score > 18 indicates identity NHLNNEGLDK + 2 Deamidated (NQ)
4479 985.8158 2954.4256 2954.4089 5.66 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
SQGMKNGVLAIPGVAFMPGNEQTCYIR + Deamidated (NQ); Oxidation (M)
1337 877.4305 876.4232 876.4341 -12.4 0 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
LSPFEQR + Deamidated (NQ)
3698 670.3311 2007.9715 2007.9935 -11.0 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
VNTNMVSGGGANIGPSFLKK + 2 Deamidated (NQ); Oxidation (M)
934 667.3530 666.3457 666.3561 -15.6 0 2 1.9 +1Score > 18 indicates identity LNHQR
1042 367.1911 732.3676 732.3806 -17.7 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
ATLWDK
1176 802.4332 801.4259 801.4418 -19.9 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
INMPLAK + Oxidation (M)
1962 518.2840 1034.5534 1034.5556 -2.07 1 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
MVHSLHRR
827 584.3412 583.3339 583.3442 -17.6 0 2 0.6 +1Score > 13 indicates identity VAAPAR
3101 514.5887 1540.7443 1540.7272 11.1 0 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
YYMDALYAFQLK + Oxidation (M)
3189 800.9018 1599.7890 1599.7675 13.5 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
RVEHASLPSNSQMK + Deamidated (NQ); Oxidation (M)
3235 815.9032 1629.7918 1629.7861 3.53 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
FIVGMEFPNKEFR + Deamidated (NQ); Oxidation (M)
4618 1111.5300 3331.5682 3331.5666 0.46 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
SGHEQVVNAKINEYYIVNGCSPLDLEQPR + 3 Deamidated (NQ)
2062 1058.4938 1057.4865 1057.4975 -10.3 1 2 2 +1Score > 18 indicates identity MNLEHKDR + Oxidation (M)
2676 604.8203 1207.6260 1207.6350 -7.38 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
SPLQGFGLFSR
2104 533.7614 1065.5082 1065.5090 -0.75 1 2 0.91 +1Score > 21 indicates identity
Score > 14 indicates homology
LGNTRNNFK + 3 Deamidated (NQ)
973 683.3751 682.3678 682.3762 -12.3 0 2 1 +1Score > 15 indicates identity TAINHK
2677 604.8212 1207.6278 1207.6350 -5.89 0 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
SPLQGFGLFSR
3867 701.3382 2100.9928 2100.9885 2.03 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
NTKNIDLMTVDSNSVTFGK + 2 Deamidated (NQ); Oxidation (M)
1245 421.7407 841.4668 841.4592 9.04 1 2 1.8 +1Score > 17 indicates identity VVKHGMR + Oxidation (M)
3129 781.8941 1561.7736 1561.7624 7.23 0 2 0.88 +1Score > 22 indicates identity
Score > 14 indicates homology
AELLSFTNNIPEGR + 2 Deamidated (NQ)
1332 875.4996 874.4923 874.4946 -2.58 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
MIKELNK
881 634.3565 633.3492 633.3486 0.98 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
KDPFK
2512 581.8158 1161.6170 1161.6063 9.23 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
VMALAAQSNLK + Deamidated (NQ); Oxidation (M)
1272 854.4122 853.4049 853.4156 -12.5 0 2 2.4 +1Score > 18 indicates identity WMLYNK
3690 669.0362 2004.0868 2004.0527 17.0 1 2 2.4 +1Score > 18 indicates identity YINGLIQQDLALDGSQKK + Deamidated (NQ)
2635 594.3177 1186.6208 1186.6194 1.26 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
VSAEQLQQVGK + Deamidated (NQ)
3856 525.2551 2096.9913 2097.0198 -13.6 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
AQAEAQARVQAQAQAEAQAR + 2 Deamidated (NQ)
3445 902.4457 1802.8768 1802.8759 0.54 1 2 0.84 +1Score > 21 indicates identity
Score > 14 indicates homology
DPRTAESGSGTTQQLVR + Deamidated (NQ)
4362 898.7781 2693.3125 2693.3636 -19.0 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
SIDTDRFLFMQNPVFQRPPELK + Oxidation (M)
3226 812.4371 1622.8596 1622.8515 5.01 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
NYGKTALGTISEELK
1963 518.2846 1034.5546 1034.5556 -0.91 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
MVHSLHRR
898 321.1850 640.3554 640.3656 -15.9 0 2 1 +1Score > 14 indicates identity VAAQPR
2663 600.2733 1198.5320 1198.5479 -13.2 0 2 2.7 +1Score > 19 indicates identity NTHSHHSGPPK + Deamidated (NQ)
1402 899.4487 898.4414 898.4508 -10.5 0 2 2.1 +1Score > 18 indicates identity NGLNPGEAK
777 513.2687 512.2614 512.2595 3.83 0 2 0.67 +1Score > 13 indicates identity GPPDK
3436 898.4585 1794.9024 1794.9186 -8.98 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
VLNVKDFSSIMAELGR + Deamidated (NQ); Oxidation (M)
4628 843.4298 3369.6901 3369.7312 -12.2 1 2 2.8 +1Score > 19 indicates identity SNILDAICFVLGISSMSTVRAQNLQDLIYK + Deamidated (NQ)
3542 940.4294 1878.8442 1878.8703 -13.8 0 2 3.1 +1Score > 19 indicates identity MEQVDLVSANMLAAGANK + 2 Deamidated (NQ); Oxidation (M)
4624 1123.2041 3366.5905 3366.5691 6.35 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
MMGTNGLPFSSVIAMLNANYMMSRLSSHYK + Oxidation (M)
3615 651.3247 1950.9523 1950.9898 -19.2 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
KPLGTELAFASEGSKSDSK
833 596.3416 595.3343 595.3442 -16.6 0 2 1 +1Score > 14 indicates identity LGPGPR
791 531.2419 530.2346 530.2336 1.85 0 2 0.69 +1Score > 13 indicates identity GPDDK
1740 494.2557 986.4968 986.4855 11.5 1 2 3.3 +1Score > 19 indicates identity KHMQDALK + Deamidated (NQ); Oxidation (M)
1666 968.4810 967.4737 967.4909 -17.8 1 2 3.2 +1Score > 19 indicates identity MVYAANKR + Oxidation (M)
1117 778.3744 777.3671 777.3691 -2.50 0 2 0.94 +1Score > 20 indicates identity
Score > 14 indicates homology
IQNMQK + Deamidated (NQ); Oxidation (M)
1884 510.2733 1018.5320 1018.5342 -2.09 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
RSLTNCLR
2590 1178.5817 1177.5744 1177.5866 -10.3 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LEALNLFNNK + 3 Deamidated (NQ)
2675 604.8189 1207.6232 1207.6350 -9.70 0 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
SPLQGFGLFSR
4373 912.4688 2734.3846 2734.3636 7.68 1 2 0.95 +1Score > 20 indicates identity
Score > 14 indicates homology
KFMSVYNPTGAEPIPQNLIINNTR + 2 Deamidated (NQ); Oxidation (M)
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