MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:29:11 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4753)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3049 505.5619 1513.6639
3050 379.4237 1513.6657
3051 757.9140 1513.8134
3052 757.9156 1513.8166
3053 758.8687 1515.7228
3055 759.3870 1516.7594
3056 759.4263 1516.8380
3060 759.8675 1517.7204
3062 760.8637 1519.7128
3063 761.3381 1520.6616
3064 761.3741 1520.7336
3065 761.8459 1521.6772
3066 762.3615 1522.7084
3067 762.3938 1522.7730
3068 762.9316 1523.8486
3070 509.5849 1525.7329
3072 510.2246 1527.6520
3073 382.9206 1527.6533
3078 766.8549 1531.6952
3079 767.3678 1532.7210
3080 767.3732 1532.7318
3082 767.3861 1532.7576
3083 767.4020 1532.7894
3084 767.4033 1532.7920
3085 767.8566 1533.6986
3086 512.2408 1533.7006
3087 768.3569 1534.6992
3088 768.3684 1534.7222
3089 768.3777 1534.7408
3090 768.4009 1534.7872
3091 512.9005 1535.6797
3092 768.8519 1535.6892
3095 769.8602 1537.7058
3097 769.8656 1537.7166
3098 769.8875 1537.7604
3099 770.3600 1538.7054
3100 770.3685 1538.7224
3102 515.2134 1542.6184
3103 773.8156 1545.6166
3105 773.9019 1545.7892
3106 516.2711 1545.7915
3107 773.9070 1545.7994
3108 774.3712 1546.7278
3109 774.3973 1546.7800
3110 774.8619 1547.7092
3111 775.3521 1548.6896
3112 775.3663 1548.7180
3113 775.3730 1548.7314
3114 775.8534 1549.6922
3115 775.8615 1549.7084
3116 776.4322 1550.8498
3117 776.8486 1551.6826
3118 776.8959 1551.7772
3119 777.8825 1553.7504
3120 778.3500 1554.6854
3121 778.3782 1554.7418
3122 778.8516 1555.6886
3123 519.9186 1556.7340
3124 779.4203 1556.8260
3125 780.3824 1558.7502
3126 780.3836 1558.7526
3127 781.3878 1560.7610
3128 781.8334 1561.6522
3130 781.8953 1561.7760
3133 521.6019 1561.7839
3135 781.9230 1561.8314
3136 782.3698 1562.7250
3139 782.8590 1563.7034
3141 785.8810 1569.7474
3142 786.3863 1570.7580
3143 786.4323 1570.8500
3144 786.9142 1571.8138
3145 786.9166 1571.8186
3146 787.3915 1572.7684
3147 787.8942 1573.7738
3148 787.9156 1573.8166
3149 787.9174 1573.8202
3150 525.9161 1574.7265
3151 788.3912 1574.7678
3152 789.3926 1576.7706
3153 789.8978 1577.7810
3154 789.8980 1577.7814
3155 789.9152 1577.8158
3156 789.9162 1577.8178
3157 789.9163 1577.8180
3158 790.8860 1579.7574
3159 791.3923 1580.7700
3160 528.2373 1581.6901
3162 793.3878 1584.7610
3163 397.4418 1585.7381
3164 529.5869 1585.7389
3165 793.8901 1585.7656
3166 794.4257 1586.8368
3167 794.9061 1587.7976
3168 530.2738 1587.7996
3169 794.9078 1587.8010
3170 794.9125 1587.8104
3171 795.3482 1588.6818
3172 795.3873 1588.7600
3173 530.8827 1589.6263
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