MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:29:11 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 4753)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1907 512.7437 1023.4728 1023.4543 18.2 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
MSNSVSSSPK + Deamidated (NQ)
1204 411.7276 821.4406 821.4317 10.9 0 5 0.46 +1Score > 21 indicates identity
Score > 14 indicates homology
LSQIMSK + Oxidation (M)
1027 725.3946 724.3873 724.3868 0.77 0 5 1 +1Score > 17 indicates identity QIAEHK
1068 744.3541 743.3468 743.3562 -12.6 0 5 0.75 +1Score > 16 indicates identity GAPQTNR + Deamidated (NQ)
963 341.1880 680.3614 680.3493 17.8 1 5 0.99 +1Score > 17 indicates identity QNKYK + Deamidated (NQ)
3699 670.3350 2007.9832 2007.9910 -3.92 1 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
SRMSVSVFFDCLYILR + Oxidation (M)
3768 687.3554 2059.0444 2059.0731 -14.0 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
MSSVAAAQNKINDLLEAIR + Oxidation (M)
2218 546.7710 1091.5274 1091.5207 6.19 1 5 0.81 +1Score > 21 indicates identity
Score > 16 indicates homology
RSSSENIDGK
805 554.3060 553.2987 553.2972 2.71 0 5 1.6 +1Score > 19 indicates identity NAPPR
1018 721.3158 720.3085 720.3112 -3.74 0 5 0.53 +1Score > 14 indicates identity CEGNIK + Deamidated (NQ)
1621 478.7368 955.4590 955.4658 -7.02 1 5 1.5 +1Score > 19 indicates identity NCEKLHR
2849 682.8069 1363.5992 1363.6150 -11.6 1 5 1.4 +1Score > 19 indicates identity SVGDAMRDLDNR + Oxidation (M)
1157 795.4116 794.4043 794.3923 15.2 0 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
EIDPHGK
2244 1098.4812 1097.4739 1097.4778 -3.50 0 4 0.93 +1Score > 17 indicates identity FFDEANAER
1502 928.4749 927.4676 927.4702 -2.74 0 4 1.2 +1Score > 18 indicates identity YETFLQK
787 529.2636 528.2563 528.2656 -17.5 0 4 0.36 +1Score > 13 indicates identity EGAPR
3547 945.4468 1888.8790 1888.8803 -0.64 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
SENDYSHKVDVNLGPSK + Deamidated (NQ)
1315 434.6971 867.3796 867.3657 16.1 0 4 0.49 +1Score > 14 indicates identity HNNHMAK + Deamidated (NQ); Oxidation (M)
1949 516.7789 1031.5432 1031.5400 3.17 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
RGPEYVSPK
4262 824.7481 2471.2225 2471.2552 -13.2 0 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
LSYNMLDTNLNSLNIALLYMR
1556 941.4437 940.4364 940.4362 0.20 0 4 1.5 +1Score > 19 indicates identity NGGSHLNNK + Deamidated (NQ)
2921 720.8623 1439.7100 1439.7256 -10.8 0 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
LGPNNDNAALEALK + Deamidated (NQ)
4736 1145.0789 4576.2865 4576.2202 14.5 1 4 0.89 +1Score > 16 indicates identity EELSNIEEDLLQPNDEMFLLASFLMNFKEITNTVISIMK + 2 Deamidated (NQ); Oxidation (M)
1433 906.4468 905.4395 905.4389 0.74 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
LSNERMR + Deamidated (NQ)
2428 573.2847 1144.5548 1144.5724 -15.3 0 4 0.6 +1Score > 21 indicates identity
Score > 15 indicates homology
ANQGTGLQDIK + Deamidated (NQ)
2753 635.8055 1269.5964 1269.6023 -4.63 1 4 1.8 +1Score > 19 indicates identity VSTDCYQRLK + Deamidated (NQ)
1210 413.7065 825.3984 825.4021 -4.41 0 4 1.4 +1Score > 18 indicates identity ELNFFR + Deamidated (NQ)
1351 441.7385 881.4624 881.4607 2.01 0 4 1.4 +1Score > 18 indicates identity QGTHLTPK + Deamidated (NQ)
1428 453.7200 905.4254 905.4137 13.0 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
NQANMRR + Deamidated (NQ); Oxidation (M)
2745 631.3343 1260.6540 1260.6574 -2.69 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
GLNNFINRANK + Deamidated (NQ)
3076 765.9091 1529.8036 1529.8300 -17.3 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
NLLDNTTLQIEKK + Deamidated (NQ)
922 329.1821 656.3496 656.3428 10.4 0 4 0.98 +1Score > 17 indicates identity MVVHR + Oxidation (M)
2756 635.8480 1269.6814 1269.6928 -8.97 1 4 0.63 +1Score > 20 indicates identity
Score > 15 indicates homology
IPVATDEEIRK
1419 903.4492 902.4419 902.4246 19.2 0 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
EADHYLR
2097 532.7737 1063.5328 1063.5298 2.85 0 4 0.69 +1Score > 21 indicates identity
Score > 15 indicates homology
QNLIFDGTR + Deamidated (NQ)
3604 971.4497 1940.8848 1940.8673 9.04 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
ESIYLMSVENGGVNGNEK + 2 Deamidated (NQ)
4773 1044.5227 6261.0925 6261.1705 -12.5 1 4 0.53 +1Score > 14 indicates identity CAEQRFTNTVQGLMILCTMCRPFLVCLGLIPQAVLSGLFFIMGIQGIIGNVIIAR + 4 Deamidated (NQ); 3 Oxidation (M)
1367 444.2147 886.4148 886.4032 13.1 0 4 1 +1Score > 17 indicates identity TGGPNSEPK + Deamidated (NQ)
3451 905.4506 1808.8866 1808.8680 10.3 0 4 0.47 +1Score > 21 indicates identity
Score > 13 indicates homology
LSDELVEIVNSGDQYK + Deamidated (NQ)
1155 794.3926 793.3853 793.3970 -14.7 0 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
NINFSAK + Deamidated (NQ)
927 331.1616 660.3086 660.3078 1.21 0 4 0.62 +1Score > 22 indicates identity
Score > 14 indicates homology
QEQQK + Deamidated (NQ)
1306 865.4074 864.4001 864.4123 -14.1 1 4 0.69 +1Score > 20 indicates identity
Score > 15 indicates homology
KEQMSSR
1262 425.2094 848.4042 848.4062 -2.27 1 4 0.43 +1Score > 21 indicates identity
Score > 13 indicates homology
KLCNQGK + 2 Deamidated (NQ)
3452 906.5016 1810.9886 1810.9829 3.19 0 4 1.6 +1Score > 19 indicates identity HNLLDNVVNVVLYGLK + 2 Deamidated (NQ)
3647 987.4800 1972.9454 1972.9378 3.90 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
LSQNDWLNIPEASDITR + 2 Deamidated (NQ)
1581 474.2335 946.4524 946.4356 17.8 0 4 0.91 +1Score > 20 indicates identity
Score > 16 indicates homology
VQESNENK
1426 453.2417 904.4688 904.4688 0.041 0 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
EGGIMGVVK + Oxidation (M)
1447 911.4614 910.4541 910.4396 15.9 0 4 1.8 +1Score > 19 indicates identity TSSSFTPGK
3075 765.9039 1529.7932 1529.7824 7.07 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
AVLESVGIEVEEEK
3233 408.1970 1628.7589 1628.7716 -7.78 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
SSEQPLDNIICQPK + Deamidated (NQ)
2429 573.2847 1144.5548 1144.5724 -15.3 1 4 0.49 +1Score > 21 indicates identity
Score > 13 indicates homology
NQEQILNKR + 3 Deamidated (NQ)
2813 664.8293 1327.6440 1327.6660 -16.5 0 4 0.73 +1Score > 20 indicates identity
Score > 15 indicates homology
TFSENYVLQVK + Deamidated (NQ)
2872 695.3854 1388.7562 1388.7412 10.8 0 4 0.83 +1Score > 20 indicates identity
Score > 16 indicates homology
IFNILGTNQVNR + Deamidated (NQ)
4094 756.3768 2266.1086 2266.1151 -2.87 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
DQIKDLDNAVNAYLMLVSSR + 2 Deamidated (NQ)
1955 517.7682 1033.5218 1033.5161 5.56 1 4 0.63 +1Score > 21 indicates identity
Score > 14 indicates homology
KPCSRCVK
920 328.1928 654.3710 654.3701 1.49 0 4 0.42 +1Score > 13 indicates identity SVPAPGK
2050 1054.5170 1053.5097 1053.5025 6.81 1 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
MSLRFDNR + Oxidation (M)
4007 1093.5425 2185.0704 2185.0395 14.2 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
LGDEKYGLSIMISSEGMSPR + Oxidation (M)
1591 949.4724 948.4651 948.4698 -4.98 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
VAAMSVAQR + Deamidated (NQ); Oxidation (M)
2678 604.8243 1207.6340 1207.6350 -0.76 0 4 2.1 +1Score > 20 indicates identity SPLQGFGLFSR
4757 1118.7423 5588.6751 5588.6463 5.15 0 4 1 +1Score > 16 indicates identity HFVPVGEYQGQLFGDSSVSPLANYVSQEEWASIISDINGLLAAAYNPVQFR + 6 Deamidated (NQ)
2598 590.2873 1178.5600 1178.5642 -3.49 0 4 0.72 +1Score > 21 indicates identity
Score > 15 indicates homology
QPSMGPTFGIK + Deamidated (NQ); Oxidation (M)
1093 764.3574 763.3501 763.3534 -4.30 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
AAVNMNK + Deamidated (NQ); Oxidation (M)
804 554.3004 553.2931 553.2972 -7.41 0 4 1.7 +1Score > 18 indicates identity NAPPR
2502 580.7850 1159.5554 1159.5543 0.98 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
MSPPGLSQEAK + Oxidation (M)
3762 1029.4890 2056.9634 2056.9297 16.4 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
DKIPDVNANGNGNVNGASNGK + 3 Deamidated (NQ)
941 669.3322 668.3249 668.3354 -15.7 1 4 1.9 +1Score > 19 indicates identity KNNHR + Deamidated (NQ)
1409 450.7249 899.4352 899.4348 0.45 0 4 1.3 +1Score > 17 indicates identity QNDAVEPK
4693 1184.5721 3550.6945 3550.6798 4.14 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
TNALIPINTASNSNSNSASSSALEINGVSFIDPNK + 4 Deamidated (NQ)
1471 460.7159 919.4172 919.3995 19.3 1 4 0.58 +1Score > 20 indicates identity
Score > 14 indicates homology
KDGSGNNAR + 2 Deamidated (NQ)
3094 769.3375 1536.6604 1536.6474 8.49 1 4 1.3 +1Score > 17 indicates identity NASLTNQDRNNCK + 3 Deamidated (NQ)
959 339.6643 677.3140 677.3054 12.8 0 3 0.51 +1Score > 21 indicates identity
Score > 13 indicates homology
MSEAPK + Oxidation (M)
1202 411.2361 820.4576 820.4443 16.3 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
DVGKQFK
1065 372.2067 742.3988 742.4086 -13.1 1 3 1.3 +1Score > 17 indicates identity AGRTGPGK
4390 922.4689 2764.3849 2764.3959 -3.99 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
QQAAETKELQQIPPQLLFVHWQK + 5 Deamidated (NQ)
1017 720.3683 719.3610 719.3602 1.13 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
WKGNSK + Deamidated (NQ)
1200 820.3843 819.3770 819.3909 -16.9 1 3 0.98 +1Score > 20 indicates identity
Score > 16 indicates homology
KMDAAER
1125 781.3943 780.3870 780.3766 13.4 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
TNLYDR
1063 743.4058 742.3985 742.3973 1.62 1 3 1.3 +1Score > 17 indicates identity QKNPEK
2386 567.7829 1133.5512 1133.5539 -2.35 0 3 0.85 +1Score > 21 indicates identity
Score > 15 indicates homology
AMAHYQGIVK + Deamidated (NQ); Oxidation (M)
3058 506.9098 1517.7076 1517.7362 -18.8 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
FNAVLENTPSSDPK
1616 477.7494 953.4842 953.4665 18.6 1 3 1.8 +1Score > 18 indicates identity KSTTTSNSK + Deamidated (NQ)
1081 756.3530 755.3457 755.3562 -13.9 0 3 1.4 +1Score > 17 indicates identity NATHQGK + Deamidated (NQ)
2046 1054.4807 1053.4734 1053.4615 11.3 0 3 1.7 +1Score > 18 indicates identity TDDSFSSAPK
4707 920.7051 3678.7913 3678.7195 19.5 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
FAQDVEAGMVWINSSNDVDINIPFGGVKMSGHGR + 2 Oxidation (M)
3358 863.4122 1724.8098 1724.8403 -17.6 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
NTMNLLANGNHPGIIK + 3 Deamidated (NQ); Oxidation (M)
3995 725.6854 2174.0344 2174.0267 3.53 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
DVTGVSFITDALDDYSQSLK + Deamidated (NQ)
4220 797.4355 2389.2847 2389.2562 11.9 1 3 1.3 +1Score > 17 indicates identity SVYVGNLNILQSIVPNMIRQK + 4 Deamidated (NQ)
2679 604.8251 1207.6356 1207.6350 0.57 0 3 2.4 +1Score > 20 indicates identity SPLQGFGLFSR
1183 810.3637 809.3564 809.3555 1.12 0 3 1.4 +1Score > 17 indicates identity GEANQYK + Deamidated (NQ)
3556 948.4896 1894.9646 1894.9458 9.92 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
GLAMSLGIGDEAGVSALYR + Oxidation (M)
4565 1039.4932 3115.4578 3115.4332 7.88 0 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
VFCTVEVADYNGIQGIWVTDESNEEIK + Deamidated (NQ)
1448 456.2364 910.4582 910.4760 -19.5 0 3 2.2 +1Score > 19 indicates identity GPIDPSTPK
1822 1006.4976 1005.4903 1005.4913 -0.97 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
AMDADIAKR + Oxidation (M)
4236 608.8110 2431.2149 2431.2165 -0.67 1 3 0.87 +1Score > 21 indicates identity
Score > 15 indicates homology
SYNGAIGVDPDKRPCGTILEAAK
1139 393.1982 784.3818 784.3755 8.03 0 3 1.6 +1Score > 18 indicates identity YFSSGPK
3660 662.3203 1983.9391 1983.9061 16.6 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
ADESKQEPNVFQTTFNK + 2 Deamidated (NQ)
2604 591.2800 1180.5454 1180.5468 -1.12 0 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
LEIMAQDSMK + Oxidation (M)
4493 742.8732 2967.4637 2967.5077 -14.8 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
AIPNGETDTWILASKNALSTLSSSYTVK + Deamidated (NQ)
3238 816.9009 1631.7872 1631.7687 11.3 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
YIQSSHVMMKFTK + Deamidated (NQ); 2 Oxidation (M)
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