MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:29:11 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 4753)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
806 555.3145 554.3072 554.3176 -18.8 0 7 0.18 +1Score > 13 indicates identity LTGHK
1609 953.4340 952.4267 952.4138 13.6 0 7 0.71 +1Score > 18 indicates identity EGGGNNIYK + 2 Deamidated (NQ)
2200 544.7735 1087.5324 1087.5397 -6.66 0 7 0.32 +1Score > 21 indicates identity
Score > 15 indicates homology
IGELNDEVAK + Deamidated (NQ)
3832 696.3525 2086.0357 2086.0106 12.0 0 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology
NFSTLTVNDIIEISYNNK + 2 Deamidated (NQ)
865 623.3515 622.3442 622.3551 -17.5 1 7 0.35 +1Score > 21 indicates identity
Score > 15 indicates homology
KHGGPK
2591 589.7945 1177.5744 1177.5696 4.15 1 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology
RQQQLMAMR + Deamidated (NQ); Oxidation (M)
855 308.6872 615.3598 615.3592 1.12 0 7 0.67 +1Score > 25 indicates identity
Score > 18 indicates homology
AVLEGK
1045 368.1754 734.3362 734.3381 -2.51 0 7 0.9 +1Score > 19 indicates identity NMANIR + Deamidated (NQ); Oxidation (M)
2409 571.3069 1140.5992 1140.6026 -2.95 1 7 0.91 +1Score > 19 indicates identity EPIQNSKVPK + 2 Deamidated (NQ)
1167 400.7171 799.4196 799.4188 1.05 0 7 0.69 +1Score > 18 indicates identity TDAIQPR
919 655.3774 654.3701 654.3701 0.081 0 7 0.19 +1Score > 13 indicates identity SVPAPGK
3476 611.9616 1832.8630 1832.8985 -19.4 1 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology
CIINQSQNSQRMLNK
1104 384.2079 766.4012 766.3861 19.8 0 7 0.63 +1Score > 20 indicates identity
Score > 18 indicates homology
QITNYK + Deamidated (NQ)
862 619.3464 618.3391 618.3449 -9.36 1 7 0.6 +1Score > 21 indicates identity
Score > 17 indicates homology
STAKGR
2904 713.9092 1425.8038 1425.8303 -18.6 1 7 0.56 +1Score > 17 indicates identity TKAVNLLTSPINR
2936 723.8718 1445.7290 1445.7548 -17.8 0 7 0.27 +1Score > 21 indicates identity
Score > 14 indicates homology
LATSTMNNLIPQK + Oxidation (M)
1225 836.4393 835.4320 835.4300 2.40 1 7 0.46 +1Score > 21 indicates identity
Score > 16 indicates homology
KGNYAQR
2326 559.7856 1117.5566 1117.5615 -4.34 1 7 1 +1Score > 22 indicates identity
Score > 19 indicates homology
LPKDSETSNK
2466 1152.5311 1151.5238 1151.5427 -16.4 0 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology
LTNSLMMNGR + Oxidation (M)
971 341.6867 681.3588 681.3520 10.1 0 7 0.77 +1Score > 18 indicates identity KPMYK + Oxidation (M)
1185 406.2207 810.4268 810.4348 -9.79 0 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology
RPQDAPK
1255 423.2226 844.4306 844.4232 8.85 0 7 0.25 +1Score > 20 indicates identity
Score > 13 indicates homology
WDFHIK
3319 847.3845 1692.7544 1692.7665 -7.11 1 7 0.88 +1Score > 19 indicates identity MSEYDKYTTPLSSR + Oxidation (M)
2206 545.2943 1088.5740 1088.5767 -2.44 0 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology
LGLGWAYGPR
1106 385.1974 768.3802 768.3766 4.73 0 7 0.84 +1Score > 19 indicates identity GVSYSTR
1431 906.4418 905.4345 905.4389 -4.79 1 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology
LSNERMR + Deamidated (NQ)
981 690.3494 689.3421 689.3530 -15.8 0 7 1 +1Score > 23 indicates identity
Score > 19 indicates homology
LTPMGR + Oxidation (M)
954 338.1771 674.3396 674.3347 7.29 0 7 0.54 +1Score > 21 indicates identity
Score > 17 indicates homology
NDALSR
2584 589.3006 1176.5866 1176.5743 10.5 1 7 0.24 +1Score > 21 indicates identity
Score > 13 indicates homology
GIERDMMGLR
2106 1066.5194 1065.5121 1065.5203 -7.67 1 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology
QQTKQYNR + Deamidated (NQ)
904 322.6723 643.3300 643.3289 1.76 0 7 0.53 +1Score > 16 indicates identity GLNPSR + Deamidated (NQ)
1764 993.4747 992.4674 992.4597 7.81 1 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology
LSDEDMRK
2202 544.7740 1087.5334 1087.5397 -5.74 0 7 0.73 +1Score > 21 indicates identity
Score > 18 indicates homology
IGELNDEVAK + Deamidated (NQ)
2725 624.3267 1246.6388 1246.6340 3.92 1 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology
VKENNVLGSCK
1889 1020.4930 1019.4857 1019.5036 -17.5 1 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology
SINEWSRK + Deamidated (NQ)
2279 1105.5751 1104.5678 1104.5597 7.33 0 6 0.92 +1Score > 21 indicates identity
Score > 19 indicates homology
INTLGGMINR + Deamidated (NQ); Oxidation (M)
2822 667.8426 1333.6706 1333.6812 -7.93 1 6 1 +1Score > 22 indicates identity
Score > 19 indicates homology
IRFQEMGLAPR + Deamidated (NQ); Oxidation (M)
1113 772.4228 771.4155 771.4239 -10.8 0 6 0.85 +1Score > 18 indicates identity VAQNAIR + Deamidated (NQ)
1179 403.6988 805.3830 805.3930 -12.3 0 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
TSQSQVR + Deamidated (NQ)
792 531.2777 530.2704 530.2700 0.79 0 6 0.23 +1Score > 13 indicates identity AAQGIA + Deamidated (NQ)
2939 723.8737 1445.7328 1445.7548 -15.2 0 6 0.31 +1Score > 21 indicates identity
Score > 14 indicates homology
LATSTMNNLIPQK + Oxidation (M)
2705 615.3012 1228.5878 1228.5796 6.72 1 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology
QQEEKSPNNR
1496 928.4130 927.4057 927.4120 -6.77 0 6 0.52 +1Score > 16 indicates identity IGSYEGMR + Oxidation (M)
2916 717.8915 1433.7684 1433.7435 17.4 1 6 1 +1Score > 21 indicates identity
Score > 19 indicates homology
KLIGMSIDNIDSK + Deamidated (NQ)
1223 418.2375 834.4604 834.4599 0.60 1 6 0.3 +1Score > 20 indicates identity
Score > 13 indicates homology
KTPQSFK
831 588.2993 587.2920 587.3027 -18.2 0 6 0.72 +1Score > 17 indicates identity NINAR + Deamidated (NQ)
2576 588.7815 1175.5484 1175.5611 -10.8 0 6 1 +1Score > 21 indicates identity
Score > 19 indicates homology
WAPVNSPNYK + Deamidated (NQ)
2915 717.8914 1433.7682 1433.7435 17.2 1 6 1 +1Score > 21 indicates identity
Score > 19 indicates homology
KLIGMSIDNIDSK + Deamidated (NQ)
1584 474.2384 946.4622 946.4607 1.61 0 6 0.67 +1Score > 21 indicates identity
Score > 17 indicates homology
IEVSEAGDK
1186 406.2251 810.4356 810.4236 14.9 0 6 1.2 +1Score > 19 indicates identity LGLHEDK
829 586.3203 585.3130 585.3122 1.37 0 6 0.8 +1Score > 17 indicates identity AGVDPK
1108 385.6973 769.3800 769.3718 10.7 1 6 0.88 +1Score > 18 indicates identity ENKNHK + Deamidated (NQ)
1137 785.3788 784.3715 784.3755 -5.13 0 6 0.83 +1Score > 18 indicates identity YFSSGPK
794 535.2516 534.2443 534.2472 -5.34 0 6 1.1 +1Score > 19 indicates identity SMGPK + Oxidation (M)
2091 532.2684 1062.5222 1062.5346 -11.6 0 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology
AVNVAPSDYK
3004 743.3740 1484.7334 1484.7405 -4.78 1 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology
IPSQRTTIHEMR + Deamidated (NQ); Oxidation (M)
1381 892.4641 891.4568 891.4484 9.47 0 6 0.5 +1Score > 22 indicates identity
Score > 15 indicates homology
GITLSNMR + Deamidated (NQ)
926 329.1832 656.3518 656.3493 3.83 0 6 0.81 +1Score > 17 indicates identity VQPEGK
2478 385.5409 1153.6009 1153.6091 -7.15 0 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology
ANVVPGTNKPR + 2 Deamidated (NQ)
903 322.6723 643.3300 643.3289 1.76 0 6 0.67 +1Score > 16 indicates identity GLNPSR + Deamidated (NQ)
1143 787.3635 786.3562 786.3620 -7.38 0 6 0.55 +1Score > 16 indicates identity NTGQDPR
3594 968.4534 1934.8922 1934.8931 -0.45 1 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology
ELEQTLPNSKEPFDMR + 2 Deamidated (NQ)
2517 582.2987 1162.5828 1162.6016 -16.1 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology
TLKGVMDNIR + Deamidated (NQ); Oxidation (M)
1064 372.2067 742.3988 742.4086 -13.1 1 6 0.77 +1Score > 17 indicates identity AGRTGPGK
1550 470.2366 938.4586 938.4457 13.8 1 6 1.4 +1Score > 20 indicates identity RGEFNSTK + Deamidated (NQ)
2533 583.2847 1164.5548 1164.5662 -9.75 0 5 0.63 +1Score > 21 indicates identity
Score > 16 indicates homology
ILNLYNQQR + 4 Deamidated (NQ)
878 630.3257 629.3184 629.3207 -3.58 0 5 0.35 +1Score > 13 indicates identity CAIAPV
3802 1038.0563 2074.0980 2074.0596 18.5 1 5 1.4 +1Score > 19 indicates identity YHSILTRTDTFDTVIHR
1296 431.2146 860.4146 860.4239 -10.8 0 5 0.69 +1Score > 21 indicates identity
Score > 16 indicates homology
QLQESEK
3134 781.9019 1561.7892 1561.7624 17.2 0 5 0.34 +1Score > 22 indicates identity
Score > 13 indicates homology
AELLSFTNNIPEGR + 2 Deamidated (NQ)
1590 949.4658 948.4585 948.4512 7.72 1 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology
ISENSKDR + Deamidated (NQ)
1508 465.7310 929.4474 929.4351 13.3 0 5 0.86 +1Score > 17 indicates identity VGLMYMGK + 2 Oxidation (M)
1597 475.7388 949.4630 949.4539 9.66 0 5 0.36 +1Score > 21 indicates identity
Score > 13 indicates homology
LGCADSSLK
3428 892.9320 1783.8494 1783.8736 -13.5 0 5 1 +1Score > 21 indicates identity
Score > 18 indicates homology
LAAMLDYNLEMLVGSK + Deamidated (NQ); Oxidation (M)
2884 700.3747 1398.7348 1398.7606 -18.4 0 5 1.5 +1Score > 19 indicates identity SLDEELIIGSPVK
1214 828.4419 827.4346 827.4501 -18.7 0 5 1 +1Score > 18 indicates identity TGTPSPLR
2793 653.8458 1305.6770 1305.6929 -12.1 0 5 0.46 +1Score > 21 indicates identity
Score > 14 indicates homology
ENIQSIQVTFK
1698 490.2278 978.4410 978.4440 -3.02 1 5 1.4 +1Score > 19 indicates identity ANAGNKMQK + 2 Deamidated (NQ); Oxidation (M)
3735 1020.9894 2039.9642 2039.9912 -13.2 0 5 1 +1Score > 21 indicates identity
Score > 18 indicates homology
IETPEQQQHNHLSVPGPK + 2 Deamidated (NQ)
4170 775.7148 2324.1226 2324.1318 -3.97 1 5 1 +1Score > 21 indicates identity
Score > 18 indicates homology
CLSSAKQTDSVINANIAFQEK + Deamidated (NQ)
1244 842.4384 841.4311 841.4341 -3.52 1 5 0.87 +1Score > 17 indicates identity CLHRTR
2233 548.2568 1094.4990 1094.5026 -3.27 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
GSTANMSLGGGK + Oxidation (M)
4676 1167.5460 3499.6162 3499.5553 17.4 1 5 1.6 +1Score > 19 indicates identity VNTNMNVNNMNVGSIPESSGVRHDQTNNNGNR + Deamidated (NQ); Oxidation (M)
3500 923.4495 1844.8844 1844.8682 8.81 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
VTNMAQAMVTSLGMSKK + Deamidated (NQ); 3 Oxidation (M)
3679 998.4723 1994.9300 1994.9181 6.00 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
RIQEQNNSVEIYSDGNK + 2 Deamidated (NQ)
3071 764.3559 1526.6972 1526.7001 -1.86 0 5 0.38 +1Score > 20 indicates identity
Score > 13 indicates homology
EQELEHPFNNIR + 2 Deamidated (NQ)
2668 601.7924 1201.5702 1201.5938 -19.6 1 5 1.4 +1Score > 19 indicates identity ANNGAKQEIEK + Deamidated (NQ)
1069 744.3647 743.3574 743.3715 -18.9 0 5 0.9 +1Score > 17 indicates identity EGHFVR
1599 475.7454 949.4762 949.4902 -14.7 1 5 0.56 +1Score > 21 indicates identity
Score > 15 indicates homology
KNMTSLQK + Deamidated (NQ)
1011 356.6844 711.3542 711.3452 12.7 0 5 1.2 +1Score > 18 indicates identity WNQHK
1693 488.7653 975.5160 975.4985 18.0 0 5 0.39 +1Score > 22 indicates identity
Score > 13 indicates homology
LGASLANSSR + Deamidated (NQ)
2335 560.7786 1119.5426 1119.5481 -4.91 1 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
QVLMEEKDK + Deamidated (NQ)
2750 633.3370 1264.6594 1264.6623 -2.24 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
RLSIVSSDSSSK
1877 1018.4888 1017.4815 1017.4951 -13.4 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
NGQATTRNR + Deamidated (NQ)
2451 1149.5569 1148.5496 1148.5309 16.3 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
GLGTSTQQEAR + 2 Deamidated (NQ)
2743 631.3195 1260.6244 1260.6020 17.8 0 5 0.49 +1Score > 22 indicates identity
Score > 14 indicates homology
GPQQQLIMSDK + Deamidated (NQ); Oxidation (M)
1134 392.2218 782.4290 782.4286 0.55 1 5 0.93 +1Score > 17 indicates identity KLEEHK
893 321.1723 640.3300 640.3293 1.24 0 5 1.3 +1Score > 18 indicates identity LHSQR + Deamidated (NQ)
1141 786.4711 785.4638 785.4647 -1.12 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
VDVIVNK
1545 939.4179 938.4106 938.3941 17.6 0 5 0.78 +1Score > 16 indicates identity STNDNSSSK
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