MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:29:11 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4753)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1630 960.5312 959.5239 959.5328 -9.23 0 20 0.074 +1Score > 21 indicates identity FVQEVLPK + Deamidated (NQ)
2974 487.6018 1459.7836 1459.8068 -15.9 1 20 0.064 +1Score > 20 indicates identity LNIDRLMELSLK + Oxidation (M)
1281 428.7306 855.4466 855.4450 1.92 0 17 0.067 +1Score > 18 indicates identity AQGQPNIK + Deamidated (NQ)
864 311.1537 620.2928 620.2952 -3.76 0 17 0.11 +1Score > 19 indicates identity MLQGR + Deamidated (NQ); Oxidation (M)
906 324.1721 646.3296 646.3286 1.67 1 16 0.23 +1Score > 23 indicates identity
Score > 22 indicates homology		 EKNGAK + Deamidated (NQ)
1039 366.2088 730.4030 730.3973 7.82 0 15 0.29 +1Score > 22 indicates identity IGQISGR + Deamidated (NQ)
779 515.3312 514.3239 514.3227 2.34 0 15 0.23 +1Score > 21 indicates identity AVAVR
999 352.2036 702.3926 702.3912 2.08 0 15 0.45 +1Score > 24 indicates identity VASLADK
1088 380.2045 758.3944 758.3922 2.91 0 14 0.21 +1Score > 20 indicates identity QQIENK
1013 357.2136 712.4126 712.4119 1.01 0 14 0.078 +1Score > 16 indicates identity QDLIPK
2532 583.2845 1164.5544 1164.5662 -10.1 0 14 0.27 +1Score > 21 indicates identity ILNLYNQQR + 4 Deamidated (NQ)
1149 397.2278 792.4410 792.4381 3.67 0 14 0.2 +1Score > 19 indicates identity LSFDIAK
899 641.3636 640.3563 640.3544 2.98 0 14 0.063 +1Score > 14 indicates identity KPGDPK
1142 786.4717 785.4644 785.4647 -0.36 0 14 0.074 +1Score > 21 indicates identity
Score > 15 indicates homology		 VDVIVNK
1358 442.7280 883.4414 883.4512 -11.0 1 13 0.19 +1Score > 19 indicates identity LKDHSQR + Deamidated (NQ)
1164 800.3790 799.3717 799.3864 -18.4 0 12 0.14 +1Score > 16 indicates identity VAEYYR
3956 1076.0193 2150.0240 2149.9983 12.0 1 12 0.42 +1Score > 21 indicates identity ADMARLQLTSPACTEVNEK + Deamidated (NQ); Oxidation (M)
1359 442.7282 883.4418 883.4512 -10.5 1 12 0.27 +1Score > 19 indicates identity LKDHSQR + Deamidated (NQ)
994 701.3582 700.3509 700.3504 0.79 0 12 0.14 +1Score > 16 indicates identity LNNPSR + Deamidated (NQ)
2069 529.7986 1057.5826 1057.5920 -8.84 0 11 0.6 +1Score > 22 indicates identity NFPLLNAIR + Deamidated (NQ)
1103 767.4061 766.3988 766.3861 16.6 1 11 0.28 +1Score > 20 indicates identity
Score > 18 indicates homology		 KDVYDK
2954 724.3835 1446.7524 1446.7579 -3.78 0 11 0.6 +1Score > 21 indicates identity LGATIDVEHSHLR
809 556.3209 555.3136 555.3241 -18.9 1 11 0.087 +1Score > 13 indicates identity ARGPR
2955 724.3838 1446.7530 1446.7579 -3.37 0 11 0.61 +1Score > 21 indicates identity LGATIDVEHSHLR
924 657.3579 656.3506 656.3493 1.96 0 11 0.2 +1Score > 17 indicates identity LQPGDK
1254 845.4373 844.4300 844.4290 1.19 0 11 0.49 +1Score > 20 indicates identity NVQNAGIK + 2 Deamidated (NQ)
1867 508.7574 1015.5002 1015.5087 -8.31 0 11 0.28 +1Score > 20 indicates identity
Score > 18 indicates homology		 LVWGQEER
1215 828.4426 827.4353 827.4501 -17.9 0 11 0.29 +1Score > 18 indicates identity TGTPSPLR
1554 470.7647 939.5148 939.5099 5.25 0 11 0.24 +1Score > 17 indicates identity MIPELPPK + Oxidation (M)
1165 800.3800 799.3727 799.3864 -17.1 0 10 0.22 +1Score > 16 indicates identity VAEYYR
952 337.1799 672.3452 672.3443 1.48 0 10 0.39 +1Score > 19 indicates identity SPVGDAK
2956 724.3863 1446.7580 1446.7579 0.089 0 10 0.73 +1Score > 22 indicates identity LGATIDVEHSHLR
995 351.1833 700.3520 700.3504 2.39 0 10 0.21 +1Score > 16 indicates identity LNNPSR + Deamidated (NQ)
1441 911.3939 910.3866 910.3888 -2.38 1 10 0.18 +1Score > 15 indicates identity MMKQNNK + 2 Deamidated (NQ); Oxidation (M)
4101 758.0237 2271.0493 2271.0866 -16.4 1 10 0.58 +1Score > 20 indicates identity SIEALKDLHLNDQEESSDTK
1025 724.4112 723.4039 723.4028 1.61 0 10 0.21 +1Score > 16 indicates identity LGEHIR
1055 371.1992 740.3838 740.3817 2.92 0 10 0.26 +1Score > 17 indicates identity KPDPER
3908 1061.4911 2120.9676 2120.9862 -8.73 1 10 0.51 +1Score > 20 indicates identity IQEQNNSVEIYSDGNKGPK + 2 Deamidated (NQ)
1040 367.1855 732.3564 732.3588 -3.27 0 10 0.25 +1Score > 22 indicates identity
Score > 17 indicates homology		 MGELQR
1102 767.4055 766.3982 766.3861 15.8 1 10 0.36 +1Score > 20 indicates identity
Score > 18 indicates homology		 KDVYDK
802 545.3109 544.3036 544.2969 12.4 0 10 0.57 +1Score > 23 indicates identity
Score > 20 indicates homology		 AAVER
1429 906.4333 905.4260 905.4429 -18.7 1 10 0.27 +1Score > 21 indicates identity
Score > 17 indicates homology		 KDHMFTK
951 337.1799 672.3452 672.3443 1.48 0 10 0.46 +1Score > 19 indicates identity SPVGDAK
2723 623.3365 1244.6584 1244.6547 3.03 0 10 0.16 +1Score > 22 indicates identity
Score > 14 indicates homology		 VGTAQIAMLANR + Deamidated (NQ)
851 612.3359 611.3286 611.3391 -17.2 0 10 0.11 +1Score > 13 indicates identity AVTHGK
1034 728.3969 727.3896 727.3977 -11.1 1 10 0.34 +1Score > 17 indicates identity SQPGRIA
2235 1095.5339 1094.5266 1094.5066 18.3 0 10 0.29 +1Score > 21 indicates identity
Score > 17 indicates homology		 LSDMNPQFK + Oxidation (M)
1131 783.4006 782.3933 782.3897 4.58 1 10 0.5 +1Score > 19 indicates identity KMWYR
856 616.3676 615.3603 615.3592 1.89 0 10 0.78 +1Score > 25 indicates identity
Score > 21 indicates homology		 VAGLEK
2465 576.7687 1151.5228 1151.5241 -1.06 1 10 0.65 +1Score > 20 indicates identity MSANGKQQGSK + Deamidated (NQ); Oxidation (M)
1148 397.2113 792.4080 792.4051 3.69 0 10 0.9 +1Score > 22 indicates identity LSMGIEK + Oxidation (M)
880 317.6639 633.3132 633.3156 -3.69 0 9 0.49 +1Score > 21 indicates identity
Score > 19 indicates homology		 DMAGIK
2755 635.8306 1269.6466 1269.6605 -10.9 1 9 0.61 +1Score > 20 indicates identity ISDFIKFDTGK
1205 824.4266 823.4193 823.4076 14.3 0 9 0.56 +1Score > 19 indicates identity ANDYLTK
1991 1042.5189 1041.5116 1041.5164 -4.63 1 9 0.59 +1Score > 19 indicates identity KETMYLNK + Oxidation (M)
2582 1177.5897 1176.5824 1176.5696 10.9 0 9 0.93 +1Score > 21 indicates identity DGINGVEMTLK + Deamidated (NQ)
3809 693.3287 2076.9643 2076.9772 -6.24 0 9 0.84 +1Score > 21 indicates identity SMDLLENNNTLTIPELDK + 2 Deamidated (NQ); Oxidation (M)
2968 730.3582 1458.7018 1458.6965 3.64 1 9 0.59 +1Score > 19 indicates identity HNMAKIYAGFYK + Deamidated (NQ); Oxidation (M)
1072 748.3173 747.3100 747.3221 -16.2 0 9 0.14 +1Score > 13 indicates identity ESMTHK + Oxidation (M)
916 655.3432 654.3359 654.3449 -13.7 0 9 0.34 +1Score > 17 indicates identity IGNSHK
2821 667.8414 1333.6682 1333.6812 -9.73 1 9 1 +1Score > 22 indicates identity
Score > 22 indicates homology		 IRFQEMGLAPR + Deamidated (NQ); Oxidation (M)
2267 1103.5052 1102.4979 1102.4825 14.0 0 9 0.47 +1Score > 18 indicates identity MSLNGSAHNR + Deamidated (NQ); Oxidation (M)
1084 757.4187 756.4114 756.4170 -7.38 0 9 0.33 +1Score > 17 indicates identity WNVLPK + Deamidated (NQ)
1548 470.2358 938.4570 938.4644 -7.80 1 9 0.27 +1Score > 20 indicates identity
Score > 16 indicates homology		 KVSGNFMR + Deamidated (NQ)
1494 928.4119 927.4046 927.4120 -7.97 0 9 0.26 +1Score > 16 indicates identity YDMGTVSR
1385 447.2657 892.5168 892.5243 -8.31 1 9 0.33 +1Score > 16 indicates identity APPRSPLR
3074 765.8776 1529.7406 1529.7362 2.92 0 9 0.17 +1Score > 21 indicates identity
Score > 14 indicates homology		 LGLDWTPDNVSANK + Deamidated (NQ)
4187 587.5263 2346.0761 2346.0347 17.7 0 9 0.73 +1Score > 20 indicates identity SVVQQDVPSEDEGAISEDEADK
2016 524.7764 1047.5382 1047.5349 3.21 1 9 0.28 +1Score > 22 indicates identity
Score > 16 indicates homology		 QNGVYNPKK + Deamidated (NQ)
2670 602.8374 1203.6602 1203.6459 11.9 0 9 0.57 +1Score > 19 indicates identity TLNSTVALTAGR + Deamidated (NQ)
1101 384.2062 766.3978 766.3861 15.3 0 9 0.57 +1Score > 20 indicates identity
Score > 19 indicates homology		 QITNYK + Deamidated (NQ)
2754 635.8292 1269.6438 1269.6605 -13.1 1 9 0.7 +1Score > 20 indicates identity ISDFIKFDTGK
2201 1088.5403 1087.5330 1087.5397 -6.13 0 9 0.94 +1Score > 21 indicates identity IGELNDEVAK + Deamidated (NQ)
1132 392.2042 782.3938 782.4035 -12.3 0 9 0.63 +1Score > 19 indicates identity QLETHR
4335 871.7382 2612.1928 2612.2201 -10.5 1 9 0.63 +1Score > 19 indicates identity IKLESLSQDIPNDDENTNNHSTK + Deamidated (NQ)
1033 728.3965 727.3892 727.3977 -11.6 1 9 0.44 +1Score > 17 indicates identity SQPGRIA
1307 865.4399 864.4326 864.4242 9.70 0 8 1 -1Score > 21 indicates identity
Score > 21 indicates homology		 GPAFNGFR
1519 466.2428 930.4710 930.4883 -18.5 0 8 0.21 +1Score > 21 indicates identity
Score > 14 indicates homology		 AGLNVSSQR
2199 544.7732 1087.5318 1087.5397 -7.21 0 8 0.97 +1Score > 21 indicates identity IGELNDEVAK + Deamidated (NQ)
860 309.6663 617.3180 617.3173 1.20 1 8 0.19 +1Score > 22 indicates identity
Score > 14 indicates homology		 KDIGW
896 321.1848 640.3550 640.3656 -16.5 0 8 0.22 +1Score > 14 indicates identity VAAQPR
1619 478.2519 954.4892 954.4923 -3.19 0 8 0.67 +1Score > 19 indicates identity EHFIPNAK
861 618.3314 617.3241 617.3173 11.0 1 8 1 +1Score > 23 indicates identity
Score > 21 indicates homology		 KDIGW
1382 446.7359 891.4572 891.4596 -2.67 1 8 0.5 +1Score > 22 indicates identity
Score > 18 indicates homology		 LGMRGQSK + Oxidation (M)
1516 466.2305 930.4464 930.4406 6.24 0 8 0.61 +1Score > 18 indicates identity NGKPSGNQK + 2 Deamidated (NQ)
2382 567.2900 1132.5654 1132.5434 19.5 1 8 0.48 +1Score > 21 indicates identity
Score > 17 indicates homology		 KMDNPNGIVK + 2 Deamidated (NQ); Oxidation (M)
2358 1126.5155 1125.5082 1125.4907 15.6 1 8 0.67 +1Score > 19 indicates identity MGKVMSNNGR + Deamidated (NQ); 2 Oxidation (M)
2503 1160.5629 1159.5556 1159.5410 12.6 1 8 0.25 +1Score > 21 indicates identity
Score > 14 indicates homology		 NHWNADKFK + Deamidated (NQ)
2892 704.3691 1406.7236 1406.7446 -14.9 1 8 1 +1Score > 22 indicates identity
Score > 20 indicates homology		 WVDTFELKQIK + Deamidated (NQ)
2686 608.7791 1215.5436 1215.5666 -18.9 1 8 0.5 +1Score > 17 indicates identity MGQGASSHGQKK + Deamidated (NQ)
858 618.3250 617.3177 617.3173 0.68 1 8 1 +1Score > 22 indicates identity
Score > 20 indicates homology		 KDIGW
2026 525.2929 1048.5712 1048.5852 -13.3 1 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 MVLRLDFR
1073 374.6909 747.3672 747.3625 6.29 0 8 0.28 +1Score > 20 indicates identity
Score > 15 indicates homology		 TTMPLW
2008 1047.4966 1046.4893 1046.4741 14.6 0 8 0.98 +1Score > 20 indicates identity NNSNNSIQR + Deamidated (NQ)
2792 653.8455 1305.6764 1305.6929 -12.6 1 8 0.47 +1Score > 21 indicates identity
Score > 17 indicates homology		 RVTVEDPSYLK
808 556.3101 555.3028 555.3017 2.11 0 8 0.23 +1Score > 14 indicates identity SPAPGK
2985 737.8649 1473.7152 1473.7212 -4.03 1 8 0.26 +1Score > 21 indicates identity
Score > 14 indicates homology		 KDHGDVSNQLYAK
970 682.3640 681.3567 681.3558 1.35 0 7 0.83 +1Score > 19 indicates identity QELHR
3766 687.3550 2059.0432 2059.0731 -14.6 1 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 MSSVAAAQNKINDLLEAIR + Oxidation (M)
1974 519.7480 1037.4814 1037.4890 -7.29 0 7 0.72 +1Score > 18 indicates identity GSIDEQHPR
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