MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:29:11 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 18)


Page: 1 2 Next 

+1

Accession Score Description
1 W0T3H5_KLUMD 96 Phosphoglycerate kinase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=PGK PE=3 SV=1

+2

Accession Score Description
1 HBA_HUMAN 84 Hemoglobin subunit alpha OS=Homo sapiens GN=HBA1 PE=1 SV=2

+3

Accession Score Description
1 TRYP_PIG 71 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 W0T6Z2_KLUMD 66 40S ribosomal protein S3 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=RPS3 PE=3 SV=1

+5

Accession Score Description
1 W0TBS2_KLUMD 61 SDO1-like protein YHR087W OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=RTC3 PE=4 SV=1

+6

Accession Score Description
1 W0TCR9_KLUMD 61 Inorganic pyrophosphatase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=IPP1 PE=3 SV=1

-7

Accession Score Description
1 W0T9W3_KLUMD 56 Fructose-bisphosphate aldolase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=FBA1 PE=3 SV=1
Score Mass Matches Sequences emPAI
7.1 W0T9W3_KLUMD 56 39643 1 (1) 1 (1) 0.08
Fructose-bisphosphate aldolase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=FBA1 PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2837   450.2409 1347.7009 1347.6823 13.8 0 56 1.9e-005 +1Score > 21 indicates identity U K.DFHTAIDFGVVK.V

+8

Accession Score Description
1 W0T6W1_KLUMD 43 Phosphoglycerate mutase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=GPM1 PE=3 SV=2

+9

Accession Score Description
1 W0TGV8_KLUMD 32 Elongation factor 1-alpha OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=TEF PE=3 SV=1

+10

Accession Score Description
1 W0T408_KLUMD 30 Inulinase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=INU1 PE=1 SV=1
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