MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:29:11 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–18 (out of 18)


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-11

Accession Score Description
1 W0TA84_KLUMD 29 Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=VIP1 PE=3 SV=1
Score Mass Matches Sequences emPAI
11.1 W0TA84_KLUMD 29 129426 2 (1) 1 (1) 0.03
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=VIP1 PE=3 SV=1

-2 peptide matches (1 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1053 +1 370.7011 739.3876 739.3864 1.65 0 29 0.0043 +1Score > 18 indicates identity U R.HINDLK.I + Deamidated (NQ)

+12

Accession Score Description
1 W0T7K9_KLUMD 25 phosphopyruvate hydratase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=ENO PE=3 SV=1

+13

Accession Score Description
1 W0T4P9_KLUMD 21 description

+14

Accession Score Description
1 W0TCN4_KLUMD 21 tRNA pseudouridine synthase 1 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=PUS1 PE=3 SV=1

+15

Accession Score Description
1 W0T8P3_KLUMD 20 Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR3 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=MGR3 PE=4 SV=1

+16

Accession Score Description
1 CAH2_HUMAN 19 Carbonic anhydrase 2 OS=Homo sapiens GN=CA2 PE=1 SV=2

+17

Accession Score Description
1 W0TBW2_KLUMD 18 Uncharacterized protein YJR141W OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=KLMA_40489 PE=4 SV=1

+18

Accession Score Description
1 W0T9E0_KLUMD 14 Glyceraldehyde-3-phosphate dehydrogenase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=GAP1 PE=3 SV=1
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