MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:28:59 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 4691)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4384 982.4807 2944.4203 2944.4240 -1.26 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
MPMPNPMAKATPMAITIPIEKPMETR + Deamidated (NQ); 3 Oxidation (M)
1090 321.1849 640.3552 640.3656 -16.2 0 2 1 +1Score > 14 indicates identity VAAQPR
2157 1061.5056 1060.4983 1060.4897 8.12 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
NGSQQQNKR + 2 Deamidated (NQ)
3080 781.8938 1561.7730 1561.7624 6.84 0 2 0.89 +1Score > 22 indicates identity
Score > 14 indicates homology
AELLSFTNNIPEGR + 2 Deamidated (NQ)
1568 889.3995 888.3922 888.4049 -14.3 0 2 1.2 +1Score > 15 indicates identity QNSEQQR
1990 510.2739 1018.5332 1018.5342 -0.92 1 2 0.83 +1Score > 20 indicates identity
Score > 13 indicates homology
RSLTNCLR
2649 1190.5712 1189.5639 1189.5575 5.43 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
SATQQQQQLR + 3 Deamidated (NQ)
1097 644.3317 643.3244 643.3289 -6.99 0 2 1.1 +1Score > 14 indicates identity GLNPSR + Deamidated (NQ)
3117 794.4167 1586.8188 1586.8403 -13.5 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
TIAETLAEELINAAK + Deamidated (NQ)
3165 810.4099 1618.8052 1618.7991 3.82 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
NLITLANYYYSQR + Deamidated (NQ)
2783 660.8276 1319.6406 1319.6568 -12.3 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
NAVNSKGLVSSNK + 3 Deamidated (NQ)
4224 885.4553 2653.3441 2653.3096 13.0 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
GVQHVPNEELIFRIENSSLSNNR + 2 Deamidated (NQ)
1772 477.7503 953.4860 953.5004 -15.1 0 1 2.8 +1Score > 19 indicates identity VTNPMPAPK
2718 626.8150 1251.6154 1251.6169 -1.16 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LEPALGMNTYK + Oxidation (M)
4131 809.0316 2424.0730 2424.1114 -15.9 1 1 2.6 +1Score > 18 indicates identity HNDENLISKMTAIEEELDHGK + 2 Deamidated (NQ)
1289 755.3596 754.3523 754.3609 -11.4 0 1 1.8 +1Score > 16 indicates identity QNLNHK + 2 Deamidated (NQ)
1485 426.2360 850.4574 850.4661 -10.2 1 1 0.99 +1Score > 20 indicates identity
Score > 14 indicates homology
RTGVSFGK
2914 723.8739 1445.7332 1445.7548 -14.9 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LAAMVNNEAKLQK + Deamidated (NQ); Oxidation (M)
4525 1136.5536 3406.6390 3406.5825 16.6 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
QIVEQMCNAGHELAAIFMSGGQCRNGLLMR + Oxidation (M)
3079 778.8474 1555.6802 1555.6611 12.3 1 1 2.5 +1Score > 18 indicates identity NGNGNGGRTDDHDVK + Deamidated (NQ)
958 529.2632 528.2559 528.2656 -18.3 0 1 0.72 +1Score > 13 indicates identity NNGPK
1616 454.7312 907.4478 907.4399 8.73 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
NASFVNVR + 2 Deamidated (NQ)
2850 695.8749 1389.7352 1389.7537 -13.3 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
QGVIQLSMIEKK + Deamidated (NQ); Oxidation (M)
2453 571.3064 1140.5982 1140.5775 18.2 1 1 3.3 +1Score > 19 indicates identity DISHLDNAKK + Deamidated (NQ)
1400 814.5010 813.4937 813.4960 -2.76 0 1 3.1 +1Score > 19 indicates identity ELLAQLK
1405 820.4280 819.4207 819.4338 -15.9 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
SKDELTK
1321 386.2254 770.4362 770.4286 9.90 1 1 1.7 +1Score > 16 indicates identity IPEEKR
2834 690.3298 1378.6450 1378.6286 12.0 0 1 0.83 +1Score > 20 indicates identity
Score > 13 indicates homology
LSNQMGTGVNINK + 4 Deamidated (NQ)
2470 573.3130 1144.6114 1144.6088 2.34 1 1 0.77 +1Score > 22 indicates identity
Score > 13 indicates homology
QKTENIINGK + Deamidated (NQ)
4041 1151.5695 2301.1244 2301.1131 4.94 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
GNNNINAKINGNNTLTNMINR + 2 Deamidated (NQ)
3990 750.3734 2248.0984 2248.0762 9.87 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
MNETLMQQQRELQMALQR + Deamidated (NQ)
2673 604.8187 1207.6228 1207.6350 -10.0 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
SPLQGFGLFSR
3924 725.0399 2172.0979 2172.0918 2.78 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
IVNHMIIDSLNEMQIKNK + Deamidated (NQ); 2 Oxidation (M)
2861 701.3397 1400.6648 1400.6684 -2.57 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
FGAPQDLQPGQSR + Deamidated (NQ)
2036 516.2873 1030.5600 1030.5481 11.6 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
APLNDVMKK + Oxidation (M)
1932 501.7626 1001.5106 1001.4964 14.2 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
QGVPMNSLR + Deamidated (NQ)
2894 717.3577 1432.7008 1432.6908 7.00 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
SVDKTFSMFLDK + Oxidation (M)
1435 833.4102 832.4029 832.4079 -5.99 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
DPAFLDR
4546 1175.5660 3523.6762 3523.7062 -8.52 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
ATNQPVLQTIQSTELTPLTLTMANDNRFMQK + 4 Deamidated (NQ); Oxidation (M)
1147 671.4226 670.4153 670.4126 4.07 0 1 1.8 +1Score > 16 indicates identity VLNAVR
3389 892.9363 1783.8580 1783.8736 -8.70 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LAAMLDYNLEMLVGSK + Deamidated (NQ); Oxidation (M)
1434 417.2085 832.4024 832.4187 -19.5 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
LMPMTPK + Oxidation (M)
2173 533.2672 1064.5198 1064.5026 16.2 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
DYENVSLPK + Deamidated (NQ)
3035 509.2599 1524.7579 1524.7613 -2.24 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
FFDEAPVLFVEGR
4536 1144.5928 3430.7566 3430.7974 -11.9 0 1 3.4 +1Score > 19 indicates identity LAQIQFWLIFVGANVIFLPMHFLGVNGMPR + 3 Deamidated (NQ)
1610 453.7198 905.4250 905.4137 12.5 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
NQANMRR + Deamidated (NQ); Oxidation (M)
4494 1090.1932 3267.5578 3267.5558 0.60 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
QGQHIFNFPHEESGAQQNQLQQKQQYR
2519 385.5410 1153.6012 1153.6091 -6.89 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
ANVVPGTNKPR + 2 Deamidated (NQ)
3131 797.3892 1592.7638 1592.7360 17.5 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
TKAEMNMLMNLHK + Deamidated (NQ); 2 Oxidation (M)
2889 716.8205 1431.6264 1431.6113 10.6 0 1 2.1 +1Score > 17 indicates identity QQQAQAQAEAQAR + 5 Deamidated (NQ)
4232 898.4481 2692.3225 2692.3151 2.73 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
LSTPQRNNLSASTSSSTLSTGDNKPK + 2 Deamidated (NQ)
3931 726.3973 2176.1701 2176.1602 4.53 1 1 2.5 +1Score > 18 indicates identity DIVLDAFLPIAFRVTPMDK + Oxidation (M)
1440 835.4360 834.4287 834.4388 -12.1 0 1 0.87 +1Score > 21 indicates identity
Score > 13 indicates homology
GWIQGFK
3602 661.9889 1982.9449 1982.9692 -12.3 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
MNQEIMSLSNILIYEGK + Deamidated (NQ)
4289 931.1840 2790.5302 2790.4843 16.4 1 1 1.4 +1Score > 15 indicates identity GSVPVYWAELNNLKYKPQLLLSEK + 2 Deamidated (NQ)
4250 912.7610 2735.2612 2735.3007 -14.4 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
YVSIEACATMGVVSNVDHQNRSLGK + Deamidated (NQ)
1177 685.3902 684.3829 684.3806 3.35 0 1 1.3 +1Score > 15 indicates identity ANVPVGK + Deamidated (NQ)
1339 784.3488 783.3415 783.3286 16.5 0 1 1.5 +1Score > 15 indicates identity ESEEYK
1145 336.2136 670.4126 670.4014 16.8 1 1 3 +1Score > 18 indicates identity AVPKEK
2808 671.8506 1341.6866 1341.7001 -10.0 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
TVSGGLRGPNVQR + 2 Deamidated (NQ)
3077 778.3840 1554.7534 1554.7388 9.42 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
MVWSVYLNELER + Deamidated (NQ); Oxidation (M)
1998 511.7451 1021.4756 1021.4577 17.6 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
RGSGPYDDR
4216 873.4280 2617.2622 2617.2768 -5.58 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
GSMADVPADLMEQIHGLETLFTVK + Oxidation (M)
2532 1158.5552 1157.5479 1157.5313 14.4 0 1 3.5 +1Score > 19 indicates identity DSPANQEEIR
3182 543.9279 1628.7619 1628.7538 4.97 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
MLGSCYERELLNK + Deamidated (NQ); Oxidation (M)
1291 756.3533 755.3460 755.3562 -13.5 0 1 2.4 +1Score > 17 indicates identity NATHQGK + Deamidated (NQ)
1146 336.2138 670.4130 670.4014 17.4 1 1 3.1 +1Score > 18 indicates identity AVPKEK
1023 594.2900 593.2827 593.2809 3.05 0 1 2.2 +1Score > 17 indicates identity GNFQK + Deamidated (NQ)
3410 605.2985 1812.8737 1812.8981 -13.5 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LGPNYLHIPVNCPYR + Deamidated (NQ)
1401 409.6671 817.3196 817.3089 13.1 0 1 0.81 +1Score > 13 indicates identity NGNGNNPK + 4 Deamidated (NQ)
2408 565.2991 1128.5836 1128.5637 17.7 0 1 4 +1Score > 19 indicates identity MLINYYANK
1648 460.2502 918.4858 918.4732 13.8 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
ADLMLDIL + Oxidation (M)
2692 611.8093 1221.6040 1221.5877 13.4 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
LGGGTYVPDQSK + Deamidated (NQ)
2705 615.7855 1229.5564 1229.5724 -13.0 1 1 2.6 +1Score > 17 indicates identity GWGCHTSAKAR
1155 678.3231 677.3158 677.3166 -1.20 0 1 0.97 +1Score > 21 indicates identity
Score > 13 indicates homology
NQMIR + Deamidated (NQ); Oxidation (M)
1949 1008.4860 1007.4787 1007.4593 19.3 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
MASLQAENK + Deamidated (NQ); Oxidation (M)
4095 784.7023 2351.0851 2351.0911 -2.55 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
AQSISSNGSPLTMNVPASSTTQR + 2 Deamidated (NQ); Oxidation (M)
1503 861.4131 860.4058 860.3988 8.18 0 1 3.3 +1Score > 19 indicates identity GLEGGNASR + Deamidated (NQ)
1906 498.7434 995.4722 995.4667 5.55 0 1 3.6 +1Score > 19 indicates identity MLEAMELK + 2 Oxidation (M)
4180 844.7719 2531.2939 2531.3358 -16.6 1 1 3.9 +1Score > 19 indicates identity IVYERIAPALCNLAFVHASYPK
4251 912.7921 2735.3545 2735.3476 2.52 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
KFMSVYNPTGAEPIPQNLIINNTR + 3 Deamidated (NQ); Oxidation (M)
2998 752.8969 1503.7792 1503.7528 17.6 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
RIQNISNESSINK + 2 Deamidated (NQ)
1717 939.4657 938.4584 938.4644 -6.34 1 1 4.1 +1Score > 20 indicates identity KVSGNFMR + Deamidated (NQ)
2190 535.2612 1068.5078 1068.5200 -11.3 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
EPSQAAVDPR
4540 861.6827 3442.7017 3442.7127 -3.20 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
MSMLAQIKQICIIASVDHIQAPFLWDHFK + 3 Deamidated (NQ)
2817 451.8985 1352.6737 1352.6798 -4.55 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
EHMEKIYLFK + Oxidation (M)
3436 919.8881 1837.7616 1837.7709 -5.05 0 1 1.2 +1Score > 14 indicates identity MEDDLENAGNEAISAMK + Deamidated (NQ)
1976 508.2662 1014.5178 1014.5346 -16.5 0 1 4.1 +1Score > 19 indicates identity IVQNEIDGK
2647 1190.5614 1189.5541 1189.5575 -2.81 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
SATQQQQQLR + 3 Deamidated (NQ)
3099 787.8971 1573.7796 1573.8100 -19.3 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
SDPIGIAYLNQNLR + Deamidated (NQ)
2696 611.8306 1221.6466 1221.6353 9.28 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
EPGNGHLEKIK + Deamidated (NQ)
1246 363.7120 725.4094 725.4072 3.13 0 1 2 +1Score > 16 indicates identity QPSVAPK
1786 480.7679 959.5212 959.5162 5.30 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
NHIRQHR
2827 687.8698 1373.7250 1373.7051 14.5 1 1 0.88 +1Score > 21 indicates identity
Score > 13 indicates homology
ESLPVFQNRER
3471 623.0381 1866.0925 1866.0574 18.8 1 1 0.84 +1Score > 13 indicates identity VANRLLITGTPLQNNIK + 2 Deamidated (NQ)
1543 440.2342 878.4538 878.4684 -16.5 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
WIASKMK + Oxidation (M)
2427 567.7832 1133.5518 1133.5452 5.88 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
LVTDNSVEEK + Deamidated (NQ)
4386 982.8149 2945.4229 2945.4481 -8.56 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
GYIIGDSLKHLQLDVPTIQGFDEVQC + Deamidated (NQ)
4458 1032.4622 3094.3648 3094.3199 14.5 1 1 3.2 +1Score > 18 indicates identity NNGSSMSPTGLTMGVTMGMGVTKSNSNSNSK + 3 Deamidated (NQ); Oxidation (M)
4513 1123.2120 3366.6142 3366.5691 13.4 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
MMGTNGLPFSSVIAMLNANYMMSRLSSHYK + Oxidation (M)
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