MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:28:59 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4691)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1370 800.3824 799.3751 799.3864 -14.1 0 3 1.2 +1Score > 16 indicates identity VAEYYR
4338 953.4766 2857.4080 2857.4095 -0.55 1 3 0.58 +1Score > 21 indicates identity
Score > 13 indicates homology
VELPEEMGIQDYTIGFAQKQFSVVK + 2 Deamidated (NQ)
1573 890.4432 889.4359 889.4505 -16.4 0 3 0.56 +1Score > 22 indicates identity
Score > 13 indicates homology
STVQDIAR + Deamidated (NQ)
3814 1050.0021 2097.9896 2098.0113 -10.3 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
NQVLVISGPGNNGGDGLVCAR + 2 Deamidated (NQ)
4058 770.3903 2308.1491 2308.1158 14.4 1 3 0.59 +1Score > 21 indicates identity
Score > 13 indicates homology
FSETGSFSSRFVTCVVSGNLK
2368 560.7740 1119.5334 1119.5230 9.32 0 3 0.74 +1Score > 21 indicates identity
Score > 14 indicates homology
SQVLECQQK + Deamidated (NQ)
1290 755.3965 754.3892 754.3973 -10.7 1 3 1.3 +1Score > 17 indicates identity KDLNHK + Deamidated (NQ)
1475 848.3976 847.3903 847.3858 5.34 0 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
MSPQDVR + Oxidation (M)
2857 700.3724 1398.7302 1398.7177 8.99 1 3 2.4 +1Score > 19 indicates identity NEMSPLKAGVPGAK + Deamidated (NQ)
3212 824.9062 1647.7978 1647.7926 3.17 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
TKEMLQFIHEAQR + 2 Deamidated (NQ); Oxidation (M)
1816 486.2814 970.5482 970.5447 3.62 0 3 1.8 +1Score > 18 indicates identity NVAVVDINK
1477 848.4152 847.4079 847.4109 -3.55 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
SLPMQEK + Oxidation (M)
2813 674.8338 1347.6530 1347.6591 -4.52 0 3 0.55 +1Score > 21 indicates identity
Score > 13 indicates homology
LLQMELEEAQK + Deamidated (NQ); Oxidation (M)
3645 1002.5482 2003.0818 2003.0761 2.86 1 3 2.5 +1Score > 19 indicates identity QAPDFTNKTLVMLANVLK + Deamidated (NQ)
1142 669.3336 668.3263 668.3354 -13.6 1 3 2.1 +1Score > 19 indicates identity KNNHR + Deamidated (NQ)
1397 813.4423 812.4350 812.4466 -14.2 0 3 1.4 +1Score > 17 indicates identity MPEPIVK
1504 431.2150 860.4154 860.4239 -9.86 0 3 0.54 +1Score > 21 indicates identity
Score > 13 indicates homology
QQEQSLK + Deamidated (NQ)
4052 770.0509 2307.1309 2307.1554 -10.6 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
QGSTSSNPGSVLASASILSSNKSK + Deamidated (NQ)
1292 756.3541 755.3468 755.3562 -12.4 0 3 1.4 +1Score > 17 indicates identity NATHQGK + Deamidated (NQ)
2241 542.8226 1083.6306 1083.6288 1.69 1 3 1.6 +1Score > 17 indicates identity EVKQGVVPTK
1737 472.7437 943.4728 943.4723 0.61 0 3 2.5 +1Score > 19 indicates identity ANVAINQGR + 2 Deamidated (NQ)
3403 903.3919 1804.7692 1804.7533 8.81 0 3 1.1 +1Score > 16 indicates identity ASSQSSSPNTNPHSQMK + 2 Deamidated (NQ); Oxidation (M)
3310 863.4125 1724.8104 1724.8403 -17.3 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
NTMNLLANGNHPGIIK + 3 Deamidated (NQ); Oxidation (M)
3388 892.9346 1783.8546 1783.8529 0.96 0 3 0.9 +1Score > 21 indicates identity
Score > 15 indicates homology
DFFHNNGEGPPLAELK
2349 557.2816 1112.5486 1112.5615 -11.5 0 3 2.4 +1Score > 19 indicates identity WGVPENGVVR + Deamidated (NQ)
1607 905.4769 904.4696 904.4688 0.90 0 3 0.6 +1Score > 22 indicates identity
Score > 13 indicates homology
EGGIMGVVK + Oxidation (M)
1474 848.3811 847.3738 847.3858 -14.1 0 3 1.3 +1Score > 16 indicates identity MSEAAAPR + Oxidation (M)
1853 982.5602 981.5529 981.5720 -19.4 1 3 1 +1Score > 15 indicates identity QRVVLDPR
3966 739.6545 2215.9417 2215.9774 -16.1 0 3 1.5 +1Score > 17 indicates identity ITCAICPEYDLCVPCFAK
4086 781.3718 2341.0936 2341.1116 -7.70 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
MIRCLNGMNYVSNDIPVTVK + 2 Deamidated (NQ); Oxidation (M)
3421 911.9205 1821.8264 1821.8493 -12.5 0 3 2.4 +1Score > 19 indicates identity VQINEAQEQSTAAYNR + Deamidated (NQ)
1585 448.7292 895.4438 895.4442 -0.34 1 3 0.69 +1Score > 20 indicates identity
Score > 14 indicates homology
MIKMMSR
2132 1054.4810 1053.4737 1053.4615 11.6 0 3 1.9 +1Score > 18 indicates identity TDDSFSSAPK
2489 1149.5571 1148.5498 1148.5309 16.5 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
GLGTSTQQEAR + 2 Deamidated (NQ)
907 487.2524 486.2451 486.2438 2.74 0 3 0.54 +1Score > 13 indicates identity ANPGK + Deamidated (NQ)
1252 730.3386 729.3313 729.3446 -18.2 0 3 0.62 +1Score > 13 indicates identity SNPHFK + Deamidated (NQ)
4682 1118.5424 5587.6756 5587.6623 2.38 0 3 1.2 +1Score > 16 indicates identity HFVPVGEYQGQLFGDSSVSPLANYVSQEEWASIISDINGLLAAAYNPVQFR + 5 Deamidated (NQ)
1282 748.3997 747.3924 747.3875 6.59 1 3 0.91 +1Score > 20 indicates identity
Score > 15 indicates homology
SNGGSKAK
2687 610.3245 1218.6344 1218.6357 -1.01 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
TLGTGNSWKVR + Deamidated (NQ)
1021 589.2886 588.2813 588.2867 -9.19 0 3 2.6 +1Score > 19 indicates identity AVDER
2779 659.3273 1316.6400 1316.6646 -18.6 1 3 0.76 +1Score > 20 indicates identity
Score > 14 indicates homology
LMNNIDKGLGPK + 2 Deamidated (NQ); Oxidation (M)
2815 676.8782 1351.7418 1351.7282 10.1 0 3 1.6 +1Score > 17 indicates identity LLNQVLMNHIR + 2 Deamidated (NQ)
2736 636.2980 1270.5814 1270.6050 -18.5 0 3 2.6 +1Score > 19 indicates identity YSLQMQVQMK + Oxidation (M)
4294 701.3411 2801.3353 2801.3793 -15.7 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
VTKHVQPLSNPAYDVSSELVQNMVK + 3 Deamidated (NQ); Oxidation (M)
3735 1030.5262 2059.0378 2059.0731 -17.1 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
MSSVAAAQNKINDLLEAIR + Oxidation (M)
2745 638.8350 1275.6554 1275.6347 16.3 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
NVGLSETELWK + Deamidated (NQ)
3516 638.3242 1911.9508 1911.9876 -19.3 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
IMLANHPDKGGSPYLATK
3032 762.3604 1522.7062 1522.6953 7.19 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
FHQHNFHLENAK + 2 Deamidated (NQ)
2973 745.3635 1488.7124 1488.7321 -13.2 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
NLREGGNAGFPLSR + 2 Deamidated (NQ)
1924 500.7915 999.5684 999.5713 -2.81 1 2 0.61 +1Score > 20 indicates identity
Score > 13 indicates homology
KQNQLQIK + Deamidated (NQ)
4402 742.8748 2967.4701 2967.5077 -12.7 1 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
AIPNGETDTWILASKNALSTLSSSYTVK + Deamidated (NQ)
3644 668.7007 2003.0803 2003.0687 5.76 1 2 2.7 +1Score > 19 indicates identity YINGLIQQDLALDGSQKK
3585 657.3284 1968.9634 1968.9251 19.4 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
MTQYKNPQEFINDLAR + 2 Deamidated (NQ)
980 545.2587 544.2514 544.2493 3.93 0 2 0.82 +1Score > 14 indicates identity QGDPK + Deamidated (NQ)
4248 912.4641 2734.3705 2734.3636 2.52 1 2 0.79 +1Score > 21 indicates identity
Score > 14 indicates homology
KFMSVYNPTGAEPIPQNLIINNTR + 2 Deamidated (NQ); Oxidation (M)
2056 518.2841 1034.5536 1034.5556 -1.88 1 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
MVHSLHRR
3800 696.3416 2086.0030 2086.0041 -0.53 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
LQEYMTPIQERAESTFK + Oxidation (M)
3280 852.9236 1703.8326 1703.8301 1.51 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
MANSVHPVEIRYSGK + Deamidated (NQ); Oxidation (M)
3953 734.7386 2201.1940 2201.2209 -12.2 1 2 2 +1Score > 18 indicates identity LATVTRVPVNPFDVSAIVFR + Deamidated (NQ)
1987 1019.4833 1018.4760 1018.4679 7.95 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
DETRNQQK + Deamidated (NQ)
1835 979.4491 978.4418 978.4440 -2.26 0 2 2.8 +1Score > 19 indicates identity ADGSMNLQK + Oxidation (M)
3041 765.9099 1529.8052 1529.8300 -16.2 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
NLLDNTTLQIEKK + Deamidated (NQ)
4707 908.7443 6354.1592 6354.0341 19.7 1 2 0.61 +1Score > 13 indicates identity NNFDELATLLSNYENLINDTHMIFIPGPNDPWSSMVGLGTTTLWPQKEIPSSFVQK + 3 Deamidated (NQ); 2 Oxidation (M)
1722 940.4482 939.4409 939.4522 -12.0 0 2 2.9 +1Score > 19 indicates identity NGGTDIHAR
1368 799.4156 798.4083 798.4123 -5.01 0 2 1.9 +1Score > 17 indicates identity ISTTYSK
1461 421.7409 841.4672 841.4592 9.51 1 2 1.8 +1Score > 17 indicates identity VVKHGMR + Oxidation (M)
3056 769.8671 1537.7196 1537.7381 -12.0 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
RMSQFPMQPGLSK + 2 Oxidation (M)
1501 859.4873 858.4800 858.4810 -1.18 1 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
NNELLKK + Deamidated (NQ)
2177 533.7624 1065.5102 1065.5090 1.13 1 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
LGNTRNNFK + 3 Deamidated (NQ)
1915 999.4786 998.4713 998.4821 -10.8 0 2 2.3 +1Score > 18 indicates identity NGAFIHDPK + Deamidated (NQ)
3466 931.4774 1860.9402 1860.9217 9.96 0 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
NISGTIDAPINGGIAEYR + Deamidated (NQ)
1010 584.3054 583.2981 583.3078 -16.6 1 2 0.63 +1Score > 13 indicates identity KNHGK + Deamidated (NQ)
1276 743.3547 742.3474 742.3358 15.7 0 2 0.91 +1Score > 14 indicates identity NNSHGSK
1211 702.3253 701.3180 701.3231 -7.29 0 2 0.7 +1Score > 13 indicates identity INNNPK + 3 Deamidated (NQ)
3031 762.3552 1522.6958 1522.6973 -0.97 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
ENKICDSNFPGIK + 2 Deamidated (NQ)
2801 446.2263 1335.6571 1335.6452 8.85 0 2 0.82 +1Score > 22 indicates identity
Score > 14 indicates homology
AVQESMSTLNTR
3453 618.2831 1851.8275 1851.8461 -10.1 0 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
AIGNWAPYVAGANMDASK + Deamidated (NQ); Oxidation (M)
4400 989.4772 2965.4098 2965.4127 -1.00 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
VMSSNYLSYQPAIGRNSTFVGLSDSQK + Deamidated (NQ); Oxidation (M)
1122 328.1930 654.3714 654.3701 2.10 0 2 0.65 +1Score > 13 indicates identity SVPAPGK
2976 497.5761 1489.7065 1489.7195 -8.73 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
HNGTAGSSLLASMTK + Oxidation (M)
3750 688.9825 2063.9257 2063.9139 5.71 1 2 3 +1Score > 19 indicates identity LNAKMNEIKPNDNEEMR + 3 Deamidated (NQ); Oxidation (M)
1131 665.3749 664.3676 664.3657 2.95 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
QHGVPK
2046 517.7688 1033.5230 1033.5161 6.72 1 2 0.81 +1Score > 21 indicates identity
Score > 13 indicates homology
KPCSRCVK
3578 983.4504 1964.8862 1964.8745 5.97 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
MSNNTETDKQVNIGPSGR + 2 Deamidated (NQ); Oxidation (M)
1888 496.7457 991.4768 991.4644 12.5 0 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
NNLMIADGK + Deamidated (NQ); Oxidation (M)
2626 1183.5630 1182.5557 1182.5591 -2.81 0 2 0.74 +1Score > 20 indicates identity
Score > 13 indicates homology
NEFVEMSIAK + Oxidation (M)
1902 498.2812 994.5478 994.5521 -4.28 1 2 2.5 +1Score > 18 indicates identity MLIKQSFK + Deamidated (NQ)
3538 968.5125 1935.0104 1935.0425 -16.6 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
QNIHPVITESAKTELVR + Deamidated (NQ)
2226 540.7961 1079.5776 1079.5910 -12.3 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
GRALIMTYR
2796 666.8491 1331.6836 1331.6867 -2.30 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
QAVMLALDKGNR + Deamidated (NQ); Oxidation (M)
3100 787.9014 1573.7882 1573.8100 -13.8 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
SDPIGIAYLNQNLR + Deamidated (NQ)
1166 341.2009 680.3872 680.3857 2.26 0 2 0.67 +1Score > 13 indicates identity AAPTPPK
1013 584.3411 583.3338 583.3442 -17.7 0 2 0.67 +1Score > 13 indicates identity KPPSR
4406 990.5259 2968.5559 2968.5983 -14.3 0 2 2 +1Score > 17 indicates identity NQVSLTIFALFVALIGQAFMIGASEALK + Deamidated (NQ); Oxidation (M)
1982 1018.4835 1017.4762 1017.4913 -14.8 0 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
MSTPEQAVR
2931 726.3748 1450.7350 1450.7164 12.8 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
TPRNSLDSVGSYR
2878 711.3958 1420.7770 1420.7786 -1.11 0 2 3 +1Score > 19 indicates identity QQLLSNHAILQR + Deamidated (NQ)
4466 1047.8423 3140.5051 3140.5396 -11.0 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology
HFPGELFSVAPYFQRNFPNLFVLNER + 3 Deamidated (NQ)
2372 1122.5090 1121.5017 1121.5142 -11.1 0 2 3 +1Score > 19 indicates identity KPEGWQNYT
4073 776.3727 2326.0963 2326.0812 6.49 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
SIVSGYKENSELENNDSVNVK + 2 Deamidated (NQ)
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