MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:28:59 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4691)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3533 966.4498 1930.8850
3534 645.0035 1931.9887
3535 967.9515 1933.8884
3536 967.9529 1933.8912
3537 645.9764 1934.9074
3539 646.3127 1935.9163
3540 968.9873 1935.9600
3541 646.3428 1936.0066
3542 969.4958 1936.9770
3543 969.9949 1937.9752
3545 970.9832 1939.9518
3546 972.4681 1942.9216
3547 648.6896 1943.0470
3548 972.9244 1943.8342
3549 972.9717 1943.9288
3550 649.3198 1944.9376
3551 973.9874 1945.9602
3552 974.4556 1946.8966
3554 488.2300 1948.8909
3555 488.2307 1948.8937
3556 975.4545 1948.8944
3557 975.4609 1948.9072
3558 650.9720 1949.8942
3559 976.4378 1950.8610
3560 976.4486 1950.8826
3561 651.3021 1950.8845
3562 976.4511 1950.8876
3563 976.4684 1950.9222
3564 976.4761 1950.9376
3565 976.4868 1950.9590
3566 651.6546 1951.9420
3567 976.9865 1951.9584
3568 651.6603 1951.9591
3569 976.9988 1951.9830
3570 651.6695 1951.9867
3571 977.0011 1951.9876
3572 977.9845 1953.9544
3573 977.9866 1953.9586
3574 977.9975 1953.9804
3575 654.2773 1959.8101
3579 984.4805 1966.9464
3580 984.9835 1967.9524
3581 656.9915 1967.9527
3582 984.9890 1967.9634
3583 984.9911 1967.9676
3584 656.9969 1967.9689
3586 657.3359 1968.9859
3587 985.9891 1969.9636
3588 987.9776 1973.9406
3589 987.9860 1973.9574
3590 988.4539 1974.8932
3591 659.3137 1974.9193
3592 659.3218 1974.9436
3593 494.9873 1975.9201
3594 659.6475 1975.9207
3595 989.9742 1977.9338
3596 990.4653 1978.9160
3597 991.4470 1980.8794
3598 991.4810 1980.9474
3599 661.6456 1981.9150
3600 992.0056 1981.9966
3601 992.4725 1982.9304
3603 992.4921 1982.9696
3604 992.5032 1982.9918
3605 992.9794 1983.9442
3606 992.9860 1983.9574
3607 993.4655 1984.9164
3608 993.9819 1985.9492
3609 497.5491 1986.1673
3610 994.4968 1986.9790
3611 663.6769 1988.0089
3612 995.4644 1988.9142
3613 995.4705 1988.9264
3614 995.4804 1988.9462
3615 664.0107 1989.0103
3616 664.3095 1989.9067
3617 995.9730 1989.9314
3618 498.4904 1989.9325
3619 664.3188 1989.9346
3620 995.9792 1989.9438
3621 996.4575 1990.9004
3622 664.6434 1990.9084
3623 996.4650 1990.9154
3624 996.9633 1991.9120
3625 665.3292 1992.9658
3626 997.5007 1992.9868
3627 997.9602 1993.9058
3629 998.4460 1994.8774
3630 998.4523 1994.8900
3631 665.9800 1994.9182
3633 998.4794 1994.9442
3634 998.5064 1994.9982
3635 999.4577 1996.9008
3636 667.3185 1998.9337
3637 1000.4770 1998.9394
3638 667.3373 1998.9901
3639 1000.5029 1998.9912
3640 1000.9777 1999.9408
3642 1002.4901 2002.9656
3643 1002.5429 2003.0712
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