MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:28:59 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 4691)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1683 928.4138 927.4065 927.4120 -5.92 0 5 0.69 +1Score > 16 indicates identity YDMGTVSR
2003 512.2512 1022.4878 1022.5066 -18.4 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
MNSNITSIK + Oxidation (M)
1775 478.7373 955.4600 955.4658 -5.98 1 5 1.3 +1Score > 19 indicates identity NCEKLHR
1608 453.2423 904.4700 904.4688 1.37 0 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
EGGIMGVVK + Oxidation (M)
1577 446.7365 891.4584 891.4596 -1.32 1 5 0.72 +1Score > 22 indicates identity
Score > 16 indicates homology
LGMRGQSK + Oxidation (M)
2315 553.2643 1104.5140 1104.5233 -8.39 1 5 0.95 +1Score > 20 indicates identity
Score > 17 indicates homology
NMELEARDK
2511 577.7504 1153.4862 1153.4921 -5.07 0 5 0.93 +1Score > 17 indicates identity TQMENGITSR + 2 Deamidated (NQ); Oxidation (M)
2677 607.3464 1212.6782 1212.6826 -3.59 1 5 1.6 +1Score > 19 indicates identity KNGNLDLGIIR + Deamidated (NQ)
3206 822.8383 1643.6620 1643.6872 -15.3 0 5 0.37 +1Score > 13 indicates identity EIDSEVDGMTEYQK + Deamidated (NQ)
3270 847.3845 1692.7544 1692.7665 -7.11 1 5 1.4 +1Score > 19 indicates identity MSEYDKYTTPLSSR + Oxidation (M)
4066 1161.5581 2321.1016 2321.0693 13.9 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
LSEMVVANGDDSTKPVAEDSNK + Oxidation (M)
2115 525.2886 1048.5626 1048.5665 -3.69 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
ANGLQKYVR + Deamidated (NQ)
1498 429.2083 856.4020 856.4000 2.34 0 5 1.2 +1Score > 18 indicates identity TSMLFNK + Deamidated (NQ); Oxidation (M)
1406 821.4272 820.4199 820.4191 0.97 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
RNLFDR + Deamidated (NQ)
1297 379.6939 757.3732 757.3719 1.84 0 5 1 +1Score > 17 indicates identity INPGDSR
1517 434.1959 866.3772 866.3817 -5.14 0 5 1.2 +1Score > 18 indicates identity HNNHMAK + Oxidation (M)
3632 998.4750 1994.9354 1994.9181 8.71 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
RIQEQNNSVEIYSDGNK + 2 Deamidated (NQ)
4645 1145.3274 4577.2805 4577.2238 12.4 0 5 1 +1Score > 17 indicates identity TLEETLNGSVLEPPAQVQTSSSSEEINWYSSFHGIGSKPFPK
933 499.2883 498.2810 498.2802 1.63 0 5 0.35 +1Score > 13 indicates identity GVVPQ
2117 525.2931 1048.5716 1048.5852 -12.9 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
MVLRLDFR
1809 967.4905 966.4832 966.4658 18.0 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
NQIQSFTK + 2 Deamidated (NQ)
2011 1024.5107 1023.5034 1023.5025 0.89 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
IKNDYWGK + Deamidated (NQ)
1785 960.5276 959.5203 959.5036 17.5 1 4 0.42 +1Score > 22 indicates identity
Score > 13 indicates homology
QSINNVKR + 2 Deamidated (NQ)
2840 692.3411 1382.6676 1382.6578 7.11 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
NANKIQNYNFR + 2 Deamidated (NQ)
1143 669.3584 668.3511 668.3606 -14.1 0 4 0.71 +1Score > 15 indicates identity RPDGPK
2469 573.2867 1144.5588 1144.5724 -11.8 1 4 0.46 +1Score > 21 indicates identity
Score > 14 indicates homology
NQEQILNKR + 3 Deamidated (NQ)
2005 512.2849 1022.5552 1022.5437 11.3 0 4 1.7 +1Score > 19 indicates identity QLVEFYPK
2957 739.8544 1477.6942 1477.7157 -14.5 0 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
GTTVGIMPSPMEVK + 2 Oxidation (M)
3515 956.4812 1910.9478 1910.9785 -16.0 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
GGLGNMHFLTNLIRNPR + 2 Deamidated (NQ)
1612 454.2154 906.4162 906.4303 -15.5 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
IMLQDMR + Deamidated (NQ)
1878 495.7411 989.4676 989.4665 1.14 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
LSEEDIER
2868 703.8558 1405.6970 1405.7201 -16.4 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
IGNDNIKTFNLR + 2 Deamidated (NQ)
1428 416.2151 830.4156 830.4134 2.74 1 4 0.58 +1Score > 21 indicates identity
Score > 14 indicates homology
KDESPQK
1502 860.4210 859.4137 859.4147 -1.19 1 4 0.58 +1Score > 20 indicates identity
Score > 14 indicates homology
GNNLQRR + 3 Deamidated (NQ)
2114 525.2886 1048.5626 1048.5665 -3.69 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
ANGLQKYVR + Deamidated (NQ)
1281 748.3193 747.3120 747.3221 -13.5 0 4 0.48 +1Score > 13 indicates identity ESMTHK + Oxidation (M)
1830 488.7659 975.5172 975.4985 19.2 0 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
LGASLANSSR + Deamidated (NQ)
1747 474.2411 946.4676 946.4607 7.32 0 4 0.99 +1Score > 21 indicates identity
Score > 17 indicates homology
IEVSEAGDK
1302 764.4310 763.4237 763.4228 1.18 1 4 0.49 +1Score > 20 indicates identity
Score > 13 indicates homology
NLKDFK
990 558.2901 557.2828 557.2809 3.43 0 4 0.56 +1Score > 14 indicates identity QGEPK
3287 855.3630 1708.7114 1708.7289 -10.2 1 4 0.77 +1Score > 15 indicates identity GGNSNNGSSSTGKHFQK + 3 Deamidated (NQ)
1363 398.2228 794.4310 794.4286 3.06 1 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
RYISEK
1836 490.2289 978.4432 978.4440 -0.77 1 4 1.8 +1Score > 19 indicates identity ANAGNKMQK + 2 Deamidated (NQ); Oxidation (M)
1556 441.7389 881.4632 881.4607 2.92 0 4 1.5 +1Score > 18 indicates identity QGTHLTPK + Deamidated (NQ)
2504 1151.5509 1150.5436 1150.5353 7.20 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
VTENVSASESK + Deamidated (NQ)
1980 1016.5290 1015.5217 1015.5298 -7.94 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
AEKNNVVNK + Deamidated (NQ)
2206 538.2865 1074.5584 1074.5709 -11.6 1 4 0.56 +1Score > 21 indicates identity
Score > 14 indicates homology
IEGLSKWNK + Deamidated (NQ)
2733 635.8063 1269.5980 1269.6023 -3.37 1 4 1.9 +1Score > 19 indicates identity VSTDCYQRLK + Deamidated (NQ)
2978 745.8618 1489.7090 1489.6831 17.4 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
HATQQSNTMKSEK + Deamidated (NQ)
1017 586.3901 585.3828 585.3737 15.5 0 4 0.66 +1Score > 15 indicates identity IIVEL
4558 1184.5691 3550.6855 3550.6798 1.60 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
TNALIPINTASNSNSNSASSSALEINGVSFIDPNK + 4 Deamidated (NQ)
3968 1109.0143 2216.0140 2215.9981 7.18 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
GINGLQQQQQPSQQYPDQR + 4 Deamidated (NQ)
2735 635.8490 1269.6834 1269.6928 -7.40 1 4 2.1 +1Score > 20 indicates identity IPVATDEEIRK
4557 1184.5688 3550.6846 3550.6798 1.35 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
TNALIPINTASNSNSNSASSSALEINGVSFIDPNK + 4 Deamidated (NQ)
1548 880.4176 879.4103 879.3947 17.8 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
ANGSTHHR + Deamidated (NQ)
3730 1029.4885 2056.9624 2056.9297 15.9 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
DKIPDVNANGNGNVNGASNGK + 3 Deamidated (NQ)
1032 602.3430 601.3357 601.3435 -12.9 0 4 0.75 +1Score > 21 indicates identity
Score > 15 indicates homology
LINDK
2802 669.3263 1336.6380 1336.6445 -4.84 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
MARSLSWDDLK + Oxidation (M)
4043 768.3829 2302.1269 2302.0924 15.0 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
QPNSTAGELRTESNAINQVIR + 5 Deamidated (NQ)
4641 752.1891 4507.0909 4507.0669 5.33 0 4 1.5 +1Score > 18 indicates identity VSEIQTQLPSFDESTVEAASQVGQMVSDASNQIGYLSSIGMAK + 5 Deamidated (NQ)
2022 514.2578 1026.5010 1026.4883 12.4 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
WHNQSGIGK + Deamidated (NQ)
2872 705.8613 1409.7080 1409.7224 -10.2 0 4 0.98 +1Score > 20 indicates identity
Score > 16 indicates homology
SLFNVSGMIADLK + Oxidation (M)
1301 764.4294 763.4221 763.4228 -0.91 1 4 0.47 +1Score > 21 indicates identity
Score > 13 indicates homology
NLKDFK
1472 846.4427 845.4354 845.4429 -8.85 1 4 0.67 +1Score > 23 indicates identity
Score > 14 indicates homology
KDPIMAR + Oxidation (M)
2165 532.2690 1062.5234 1062.5346 -10.5 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
AVNVAPSDYK
2271 546.7725 1091.5304 1091.5207 8.94 1 4 0.82 +1Score > 21 indicates identity
Score > 15 indicates homology
RSSSENIDGK
2863 702.3655 1402.7164 1402.6940 16.0 0 4 0.51 +1Score > 21 indicates identity
Score > 13 indicates homology
LQSVNQQLDSLR + 3 Deamidated (NQ)
1981 1017.5507 1016.5434 1016.5291 14.1 0 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
ANDVIATWK
938 502.3053 501.2980 501.2911 13.9 0 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
GANIK
2880 713.3417 1424.6688 1424.6969 -19.7 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
VAQVLQYSMNVR + 2 Deamidated (NQ); Oxidation (M)
2320 553.2930 1104.5714 1104.5710 0.43 1 4 0.55 +1Score > 21 indicates identity
Score > 13 indicates homology
RQSMVEVTR
2643 1188.6240 1187.6167 1187.6146 1.79 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
SGSNNLLDVLR + Deamidated (NQ)
1378 802.4003 801.3930 801.3868 7.75 0 4 1.9 +1Score > 19 indicates identity LNPNESK + Deamidated (NQ)
1123 657.3577 656.3504 656.3493 1.66 0 4 1.1 +1Score > 17 indicates identity LQPGDK
3770 1038.0488 2074.0830 2074.0596 11.3 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
YHSILTRTDTFDTVIHR
3110 792.8667 1583.7188 1583.7151 2.38 0 3 0.61 +1Score > 20 indicates identity
Score > 14 indicates homology
HIGGVADHNMYPTR + Deamidated (NQ); Oxidation (M)
3576 655.6341 1963.8805 1963.8905 -5.12 1 3 2 +1Score > 19 indicates identity MSNNTETDKQVNIGPSGR + Deamidated (NQ); Oxidation (M)
1076 628.3434 627.3361 627.3452 -14.5 1 3 0.97 +1Score > 16 indicates identity RAEPR
1422 414.2185 826.4224 826.4371 -17.7 0 3 2.1 +1Score > 19 indicates identity GQPIMGPK
2875 709.3248 1416.6350 1416.6534 -13.0 0 3 1.9 +1Score > 19 indicates identity ADGWISQAHYNR
3911 721.7194 2162.1364 2162.1041 14.9 1 3 2.2 +1Score > 19 indicates identity IMQYIAAASDTHEASIGKIK + Oxidation (M)
988 557.2960 556.2887 556.2969 -14.7 0 3 0.64 +1Score > 14 indicates identity VGPER
1408 411.2361 820.4576 820.4443 16.3 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
DVGKQFK
2614 590.2883 1178.5620 1178.5642 -1.79 0 3 0.77 +1Score > 21 indicates identity
Score > 15 indicates homology
QPSMGPTFGIK + Deamidated (NQ); Oxidation (M)
2693 611.8120 1221.6094 1221.6201 -8.68 1 3 0.82 +1Score > 22 indicates identity
Score > 15 indicates homology
SKSISAASTNEK
3309 863.4120 1724.8094 1724.8403 -17.9 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
NTMNLLANGNHPGIIK + 3 Deamidated (NQ); Oxidation (M)
1259 367.1909 732.3672 732.3806 -18.3 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
ATLWDK
2926 725.3505 1448.6864 1448.7115 -17.3 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
NLNIVQMMQVAR + Deamidated (NQ); 2 Oxidation (M)
2603 588.7821 1175.5496 1175.5611 -9.74 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
WAPVNSPNYK + Deamidated (NQ)
2465 572.7885 1143.5624 1143.5825 -17.6 0 3 0.83 +1Score > 20 indicates identity
Score > 15 indicates homology
NGAGFFHVAPK
3577 983.4493 1964.8840 1964.8745 4.85 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
MSNNTETDKQVNIGPSGR + 2 Deamidated (NQ); Oxidation (M)
3111 792.8883 1583.7620 1583.7427 12.2 1 3 0.99 +1Score > 21 indicates identity
Score > 16 indicates homology
QNTPLGNDIPDDRK + 2 Deamidated (NQ)
3736 687.3537 2059.0393 2059.0731 -16.4 1 3 0.58 +1Score > 21 indicates identity
Score > 13 indicates homology
MSSVAAAQNKINDLLEAIR + Oxidation (M)
3738 1030.5306 2059.0466 2059.0731 -12.9 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
MSSVAAAQNKINDLLEAIR + Oxidation (M)
1692 932.4529 931.4456 931.4610 -16.6 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
DLSEEALR
1533 877.4250 876.4177 876.4124 6.13 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
VTLCDNR
1148 671.4230 670.4157 670.4126 4.66 0 3 1.2 +1Score > 16 indicates identity VLNAVR
2686 610.3062 1218.5978 1218.6067 -7.23 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
MTPLKNNWAK + Deamidated (NQ); Oxidation (M)
3081 781.8981 1561.7816 1561.7624 12.4 0 3 0.61 +1Score > 22 indicates identity
Score > 14 indicates homology
AELLSFTNNIPEGR + 2 Deamidated (NQ)
1265 736.3653 735.3580 735.3551 3.93 0 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
GEFNLR + Deamidated (NQ)
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