MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:28:59 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 4691)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1058 615.3113 614.3040 614.3024 2.70 0 7 0.21 +1Score > 13 indicates identity NGEPAK
3040 765.9080 1529.8014 1529.8300 -18.7 1 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 NLLDNTTLQIEKK + Deamidated (NQ)
1065 619.3495 618.3422 618.3449 -4.34 1 7 0.37 +1Score > 21 indicates identity
Score > 16 indicates homology		 STAKGR
2644 594.8165 1187.6184 1187.6146 3.25 0 7 0.76 +1Score > 21 indicates identity
Score > 19 indicates homology		 SGSNNLLDVLR + Deamidated (NQ)
2310 368.5361 1102.5865 1102.6056 -17.4 0 7 0.47 +1Score > 21 indicates identity
Score > 17 indicates homology		 AGLVLQLMNK + Deamidated (NQ); Oxidation (M)
1077 629.2916 628.2843 628.2929 -13.6 0 7 0.34 +1Score > 15 indicates identity QDPNR
1206 350.7166 699.4186 699.4167 2.83 0 7 0.7 +1Score > 18 indicates identity ETPLIK
2034 516.2634 1030.5122 1030.5083 3.79 0 7 0.73 +1Score > 22 indicates identity
Score > 18 indicates homology		 FNESPVNPK
2145 1058.4951 1057.4878 1057.4975 -9.10 1 7 0.68 +1Score > 18 indicates identity MNLEHKDR + Oxidation (M)
2642 594.8128 1187.6110 1187.6146 -2.99 0 7 0.83 +1Score > 21 indicates identity
Score > 19 indicates homology		 SGSNNLLDVLR + Deamidated (NQ)
2674 604.8214 1207.6282 1207.6350 -5.56 0 7 1 +1Score > 20 indicates identity
Score > 20 indicates homology		 SPLQGFGLFSR
1060 308.6877 615.3608 615.3592 2.74 0 7 0.67 +1Score > 25 indicates identity
Score > 18 indicates homology		 AVLEGK
1104 323.1718 644.3290 644.3354 -9.86 1 7 0.76 +1Score > 18 indicates identity ADGRAR
2871 705.8550 1409.6954 1409.7038 -5.91 1 7 0.54 +1Score > 21 indicates identity
Score > 17 indicates homology		 QALEELSYSKDK
3553 650.0022 1946.9848 1946.9949 -5.20 0 7 0.26 +1Score > 20 indicates identity
Score > 14 indicates homology		 TLNIGNLTEISDELYPR
986 555.3143 554.3070 554.3176 -19.2 0 7 0.2 +1Score > 13 indicates identity LTGHK
3713 1023.0363 2044.0580 2044.0841 -12.7 1 7 0.27 +1Score > 20 indicates identity
Score > 14 indicates homology		 LYVQPAITPKSLLGDDTGR + Deamidated (NQ)
4109 593.7970 2371.1589 2371.1867 -11.7 1 7 0.27 +1Score > 22 indicates identity
Score > 14 indicates homology		 TILQELGEDTSREGLLDTPQR + Deamidated (NQ)
2971 743.3759 1484.7372 1484.7405 -2.22 1 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology		 IPSQRTTIHEMR + Deamidated (NQ); Oxidation (M)
1056 614.2803 613.2730 613.2820 -14.6 0 7 0.21 +1Score > 13 indicates identity NAGHSK + Deamidated (NQ)
1016 586.3212 585.3139 585.3122 2.90 0 7 0.66 +1Score > 17 indicates identity AGVDPK
1242 363.2014 724.3882 724.3908 -3.53 0 7 0.63 +1Score > 17 indicates identity IGNFFK
3339 873.4383 1744.8620 1744.8454 9.56 0 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 WNNQMKPLVQNLNK + 3 Deamidated (NQ); Oxidation (M)
1453 421.2448 840.4750 840.4705 5.41 0 7 0.46 +1Score > 16 indicates identity LDGLQPAK
1890 992.5450 991.5377 991.5239 13.9 0 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 WVANFLSR
2358 558.3092 1114.6038 1114.5870 15.2 0 7 0.38 +1Score > 22 indicates identity
Score > 15 indicates homology		 AQELIDNALK + Deamidated (NQ)
1055 614.2784 613.2711 613.2820 -17.7 0 7 0.22 +1Score > 13 indicates identity NAGHSK + Deamidated (NQ)
1078 315.6647 629.3148 629.3245 -15.3 0 7 0.25 +1Score > 13 indicates identity INNNR
1151 337.1798 672.3450 672.3443 1.18 0 7 0.95 +1Score > 19 indicates identity SPVGDAK
2264 545.8021 1089.5896 1089.5852 4.06 1 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 ITTKMIPDR + Oxidation (M)
1375 400.7361 799.4576 799.4552 3.10 0 7 0.92 +1Score > 20 indicates identity
Score > 19 indicates homology		 AQQIALR + Deamidated (NQ)
2791 664.8306 1327.6466 1327.6507 -3.06 0 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology		 STETSTYAELVK
1388 810.3645 809.3572 809.3555 2.11 0 6 0.65 +1Score > 17 indicates identity GEANQYK + Deamidated (NQ)
3155 805.8699 1609.7252 1609.7233 1.20 1 6 1.1 +1Score > 19 indicates identity FSNSFTTQNNHRR + 2 Deamidated (NQ)
1423 414.2219 826.4292 826.4371 -9.50 0 6 0.96 +1Score > 19 indicates identity GQPIMGPK
2760 648.8343 1295.6540 1295.6762 -17.1 0 6 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 TSFFQALGVPTK + Deamidated (NQ)
1806 483.7391 965.4636 965.4818 -18.8 0 6 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 IGYTLDQR + Deamidated (NQ)
2759 648.8340 1295.6534 1295.6762 -17.5 0 6 0.29 +1Score > 20 indicates identity
Score > 14 indicates homology		 TSFFQALGVPTK + Deamidated (NQ)
2754 644.3175 1286.6204 1286.6143 4.81 0 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology		 DIQESGNWLPK + Deamidated (NQ)
2768 654.3919 1306.7692 1306.7860 -12.8 0 6 0.23 +1Score > 13 indicates identity LLVNILPGKPNK + 2 Deamidated (NQ)
2141 528.7718 1055.5290 1055.5144 13.9 0 6 1 +1Score > 19 indicates identity QIGVMSMYK
2727 631.3196 1260.6246 1260.6020 18.0 0 6 0.36 +1Score > 22 indicates identity
Score > 14 indicates homology		 GPQQQLIMSDK + Deamidated (NQ); Oxidation (M)
2506 576.3330 1150.6514 1150.6420 8.23 1 6 0.59 +1Score > 16 indicates identity LIAKNGFMIK + Deamidated (NQ); Oxidation (M)
1253 731.3581 730.3508 730.3609 -13.8 0 6 0.68 +1Score > 17 indicates identity ENIEAR
2023 514.2758 1026.5370 1026.5498 -12.4 1 6 1 +1Score > 19 indicates identity AVVEKYYR
1232 720.3817 719.3744 719.3748 -0.55 1 6 0.87 +1Score > 21 indicates identity
Score > 18 indicates homology		 REMLR + Oxidation (M)
2610 589.7949 1177.5752 1177.5696 4.83 1 6 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 RQQQLMAMR + Deamidated (NQ); Oxidation (M)
2739 636.3207 1270.6268 1270.6227 3.26 0 6 0.81 +1Score > 20 indicates identity
Score > 18 indicates homology		 MGLEALLDEHK + Oxidation (M)
2707 617.3135 1232.6124 1232.6070 4.39 1 6 0.63 +1Score > 22 indicates identity
Score > 17 indicates homology		 MELQQKAEVR + 2 Deamidated (NQ)
1070 313.1877 624.3608 624.3707 -15.8 0 6 0.67 +1Score > 17 indicates identity APRPGK
1152 337.6620 673.3094 673.3071 3.44 0 6 0.31 +1Score > 13 indicates identity FEHLQ + Deamidated (NQ)
1307 766.3826 765.3753 765.3657 12.6 0 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology		 FNLSER + Deamidated (NQ)
3084 781.9004 1561.7862 1561.7624 15.3 0 6 0.34 +1Score > 22 indicates identity
Score > 14 indicates homology		 AELLSFTNNIPEGR + 2 Deamidated (NQ)
952 515.3212 514.3139 514.3227 -17.1 0 6 0.67 +1Score > 20 indicates identity
Score > 17 indicates homology		 AVAVR
1361 795.4126 794.4053 794.3923 16.4 0 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 EIDPHGK
1710 469.2370 936.4594 936.4553 4.47 0 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 VDGSLFNGK + Deamidated (NQ)
1600 903.5060 902.4987 902.5073 -9.48 1 6 0.54 +1Score > 22 indicates identity
Score > 16 indicates homology		 IGQQKTVK + 2 Deamidated (NQ)
1100 322.6728 643.3310 643.3289 3.31 0 6 0.57 +1Score > 16 indicates identity GLNPSR + Deamidated (NQ)
3085 781.9028 1561.7910 1561.7624 18.4 0 6 0.34 +1Score > 22 indicates identity
Score > 14 indicates homology		 AELLSFTNNIPEGR + 2 Deamidated (NQ)
3192 817.8984 1633.7822 1633.7817 0.33 1 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 LKHNSFQTICCAR
2663 601.2907 1200.5668 1200.5445 18.6 0 6 0.98 +1Score > 18 indicates identity ITQCIDNHTV + Deamidated (NQ)
1404 410.7104 819.4062 819.3915 18.0 0 6 0.42 +1Score > 21 indicates identity
Score > 15 indicates homology		 HFPYEK
2666 601.7927 1201.5708 1201.5938 -19.1 1 6 1.1 +1Score > 19 indicates identity ANNGAKQEIEK + Deamidated (NQ)
3101 787.9193 1573.8240 1573.8464 -14.2 1 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 DVIPNRNIYTEIK
1227 358.7043 715.3940 715.3864 10.7 0 6 0.29 +1Score > 21 indicates identity
Score > 13 indicates homology		 INELAR + Deamidated (NQ)
3894 1075.5015 2148.9884 2149.0143 -12.0 1 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 ADMARLQLTSPACTEVNEK + Oxidation (M)
2539 1160.5620 1159.5547 1159.5410 11.8 1 6 0.78 +1Score > 21 indicates identity
Score > 17 indicates homology		 NHWNADKFK + Deamidated (NQ)
1103 644.3638 643.3565 643.3653 -13.6 0 6 0.47 +1Score > 21 indicates identity
Score > 15 indicates homology		 GILNAR + Deamidated (NQ)
1234 721.3869 720.3796 720.3840 -6.06 1 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 LDSMKK
1753 475.7389 949.4632 949.4539 9.87 0 6 0.34 +1Score > 21 indicates identity
Score > 14 indicates homology		 LGCADSSLK
1319 771.3889 770.3816 770.3671 18.9 0 6 0.91 +1Score > 18 indicates identity VNSAHSR + Deamidated (NQ)
1364 398.7198 795.4250 795.4351 -12.6 1 6 1 +1Score > 18 indicates identity ANKAAHGK
1594 450.7253 899.4360 899.4348 1.34 0 6 0.94 +1Score > 18 indicates identity QNDAVEPK
2734 635.8320 1269.6494 1269.6605 -8.71 1 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 ISDFIKFDTGK
1605 905.4767 904.4694 904.4688 0.68 0 6 0.36 +1Score > 22 indicates identity
Score > 14 indicates homology		 EGGIMGVVK + Oxidation (M)
1864 493.2527 984.4908 984.4950 -4.21 1 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 YTKAMIDK + Oxidation (M)
1089 641.3264 640.3191 640.3254 -9.82 0 6 1 +1Score > 18 indicates identity YIAMK + Oxidation (M)
1154 339.6646 677.3146 677.3054 13.6 0 6 0.37 +1Score > 21 indicates identity
Score > 14 indicates homology		 MSEAPK + Oxidation (M)
2780 659.3299 1316.6452 1316.6394 4.40 0 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 SMPAQGVDDLIR + Oxidation (M)
2116 525.2930 1048.5714 1048.5852 -13.1 1 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 MVLRLDFR
1379 401.7256 801.4366 801.4497 -16.3 0 5 0.6 +1Score > 23 indicates identity
Score > 16 indicates homology		 WTILNR
1067 624.3356 623.3283 623.3279 0.73 0 5 1.1 +1Score > 18 indicates identity VSQYK
3858 1063.0374 2124.0602 2124.0826 -10.5 1 5 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 WLVAFLKTQMAPGSQYQR + Deamidated (NQ)
1258 367.1857 732.3568 732.3588 -2.72 0 5 0.32 +1Score > 22 indicates identity
Score > 13 indicates homology		 MDAIQR
1389 405.6860 809.3574 809.3555 2.39 0 5 0.78 +1Score > 17 indicates identity GEANQYK + Deamidated (NQ)
1393 406.2207 810.4268 810.4348 -9.79 0 5 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 RPQDAPK
1606 453.2420 904.4694 904.4800 -11.7 1 5 0.84 +1Score > 22 indicates identity
Score > 17 indicates homology		 DALATMKR
2909 482.9137 1445.7193 1445.7449 -17.7 1 5 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 TFHMKQLSNALR + Deamidated (NQ)
3628 665.6489 1993.9249 1993.9085 8.21 0 5 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 VAGQLNVMVQTMNDTSPR + 2 Deamidated (NQ); 2 Oxidation (M)
1338 783.4725 782.4652 782.4762 -14.1 0 5 0.31 +1Score > 13 indicates identity RPELIR
3204 822.3697 1642.7248 1642.7508 -15.8 0 5 1.2 +1Score > 19 indicates identity EQQNNQMQLPVAPK + 3 Deamidated (NQ); Oxidation (M)
3934 1091.9967 2181.9788 2181.9370 19.2 0 5 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 QTSSSSPATTGVGGSNGNSNSAGR + 2 Deamidated (NQ)
1020 588.3004 587.2931 587.3027 -16.3 0 5 0.88 +1Score > 17 indicates identity NINAR + Deamidated (NQ)
2892 717.3405 1432.6664 1432.6405 18.1 0 5 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 MSAGWNNAPNVEK + Oxidation (M)
3036 764.3563 1526.6980 1526.7001 -1.34 0 5 1.5 +1Score > 20 indicates identity EQELEHPFNNIR + 2 Deamidated (NQ)
1022 592.2728 591.2655 591.2612 7.24 0 5 0.61 +1Score > 16 indicates identity SDQSR
2888 716.3893 1430.7640 1430.7881 -16.8 1 5 0.34 +1Score > 21 indicates identity
Score > 13 indicates homology		 QGKLVPAGELPGHK + Deamidated (NQ)
3137 800.8676 1599.7206 1599.7376 -10.6 1 5 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 VAPKNLEEGEADDGR + Deamidated (NQ)
2778 658.8492 1315.6838 1315.6871 -2.46 0 5 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 EPISVSSEQVLK + Deamidated (NQ)
3112 793.8611 1585.7076 1585.7372 -18.6 1 5 1.4 +1Score > 19 indicates identity NHVNVNPNEKAYGK + 3 Deamidated (NQ)
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