MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:28:59 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4691)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1183 344.7165 687.4184 687.4167 2.56 0 19 0.064 +1Score > 20 indicates identity TEIVVK
1677 463.7591 925.5036 925.4943 10.1 0 18 0.053 +1Score > 18 indicates identity MPPLVPEK + Oxidation (M)
1080 316.6852 631.3558 631.3541 2.80 0 18 0.17 +1Score > 25 indicates identity
Score > 23 indicates homology		 VITGNK + Deamidated (NQ)
1066 311.1536 620.2926 620.2952 -4.08 0 17 0.11 +1Score > 19 indicates identity MLQGR + Deamidated (NQ); Oxidation (M)
2035 516.2645 1030.5144 1030.5294 -14.6 1 16 0.18 +1Score > 22 indicates identity
Score > 22 indicates homology		 LRQEDQLK + 2 Deamidated (NQ)
2561 583.2880 1164.5614 1164.5662 -4.09 0 16 0.17 +1Score > 21 indicates identity ILNLYNQQR + 4 Deamidated (NQ)
1255 366.1890 730.3634 730.3722 -11.9 1 16 0.11 +1Score > 19 indicates identity NGIRNR + 2 Deamidated (NQ)
1184 345.1956 688.3766 688.3643 17.9 0 16 0.077 +1Score > 23 indicates identity
Score > 17 indicates homology		 TLNIVE + Deamidated (NQ)
1254 731.3600 730.3527 730.3609 -11.3 0 16 0.076 +1Score > 17 indicates identity NLQGGNK + Deamidated (NQ)
1468 845.4375 844.4302 844.4290 1.43 0 16 0.17 +1Score > 20 indicates identity NVQNAGIK + 2 Deamidated (NQ)
2855 698.3723 1394.7300 1394.7558 -18.4 1 16 0.16 +1Score > 21 indicates identity
Score > 20 indicates homology		 FLALYKDEIQR
1257 366.2094 730.4042 730.3973 9.46 0 15 0.32 +1Score > 22 indicates identity IGQISGR + Deamidated (NQ)
1207 701.3593 700.3520 700.3504 2.36 0 15 0.075 +1Score > 16 indicates identity LNNPSR + Deamidated (NQ)
1874 494.7823 987.5500 987.5349 15.3 1 15 0.24 +1Score > 21 indicates identity ERDVLQTK
1367 399.7076 797.4006 797.3959 5.89 0 15 0.11 +1Score > 17 indicates identity EVEFFK
1351 393.7414 785.4682 785.4647 4.53 0 14 0.26 +1Score > 21 indicates identity IGDLAGIK
3737 687.3550 2059.0432 2059.0731 -14.6 1 13 0.33 +1Score > 21 indicates identity MSSVAAAQNKINDLLEAIR + Oxidation (M)
1544 440.2343 878.4540 878.4610 -7.91 1 13 0.25 +1Score > 21 indicates identity
Score > 20 indicates homology		 QTFKQAR + Deamidated (NQ)
1185 691.3084 690.3011 690.2933 11.4 0 13 0.13 +1Score > 17 indicates identity QNSGQR + 2 Deamidated (NQ)
2105 1048.5547 1047.5474 1047.5461 1.22 1 13 0.082 +1Score > 22 indicates identity
Score > 14 indicates homology		 GDRLGFLDR
1929 501.2885 1000.5624 1000.5441 18.4 0 13 0.28 +1Score > 22 indicates identity
Score > 20 indicates homology		 VDLIGQNLK + 2 Deamidated (NQ)
2050 518.2390 1034.4634 1034.4451 17.8 0 13 0.24 +1Score > 19 indicates identity LNNQNEMR + Deamidated (NQ); Oxidation (M)
2447 380.5409 1138.6009 1138.6233 -19.7 1 12 0.26 +1Score > 19 indicates identity QIPAENLPKK + 2 Deamidated (NQ)
1369 800.3804 799.3731 799.3864 -16.6 0 12 0.15 +1Score > 16 indicates identity VAEYYR
1897 994.4607 993.4534 993.4437 9.82 0 12 0.11 +1Score > 20 indicates identity
Score > 15 indicates homology		 NANLSMAEK + Deamidated (NQ); Oxidation (M)
1101 644.3385 643.3312 643.3289 3.58 0 12 0.14 +1Score > 16 indicates identity GLNPSR + Deamidated (NQ)
1561 442.7288 883.4430 883.4512 -9.18 1 12 0.28 +1Score > 19 indicates identity LKDHSQR + Deamidated (NQ)
3355 879.4675 1756.9204 1756.8883 18.3 0 12 0.36 +1Score > 20 indicates identity LIPSVSEDELNHYLK + Deamidated (NQ)
2816 676.8788 1351.7430 1351.7282 11.0 0 12 0.2 +1Score > 17 indicates identity LLNQVLMNHIR + 2 Deamidated (NQ)
1099 322.6723 643.3300 643.3402 -15.7 0 11 0.18 +1Score > 16 indicates identity IGQNGR
1124 329.1825 656.3504 656.3606 -15.4 0 11 0.19 +1Score > 17 indicates identity GPQLSR
2842 462.5399 1384.5979 1384.6219 -17.3 1 11 0.17 +1Score > 16 indicates identity DGEVTNNNEHKK + Deamidated (NQ)
1049 612.3376 611.3303 611.3391 -14.4 0 11 0.074 +1Score > 13 indicates identity AVTHGK
1310 767.4069 766.3996 766.3861 17.6 1 11 0.24 +1Score > 20 indicates identity
Score > 18 indicates homology		 KDVYDK
2924 724.3857 1446.7568 1446.7579 -0.74 0 11 0.61 -1Score > 22 indicates identity LGATIDVEHSHLR
2079 1042.5183 1041.5110 1041.5164 -5.21 1 11 0.38 +1Score > 19 indicates identity KETMYLNK + Oxidation (M)
1030 601.3644 600.3571 600.3595 -3.93 0 11 0.33 +1Score > 19 indicates identity NLLNK
1464 843.4942 842.4869 842.4861 0.94 0 11 0.48 +1Score > 20 indicates identity QLEAIIR + Deamidated (NQ)
2923 724.3848 1446.7550 1446.7579 -1.98 0 11 0.65 +1Score > 22 indicates identity LGATIDVEHSHLR
2478 573.7874 1145.5602 1145.5564 3.35 0 11 0.33 +1Score > 21 indicates identity
Score > 19 indicates homology		 ANQGTGLQDIK + 2 Deamidated (NQ)
3896 1076.0211 2150.0276 2149.9983 13.6 1 11 0.54 +1Score > 21 indicates identity ADMARLQLTSPACTEVNEK + Deamidated (NQ); Oxidation (M)
1098 644.3371 643.3298 643.3289 1.41 0 11 0.2 +1Score > 16 indicates identity GLNPSR + Deamidated (NQ)
2925 724.3885 1446.7624 1446.7579 3.13 0 11 0.11 +1Score > 22 indicates identity
Score > 14 indicates homology		 LGATIDVEHSHLR
966 531.2781 530.2708 530.2700 1.55 0 11 0.89 +1Score > 23 indicates identity AAQGIA + Deamidated (NQ)
2018 513.7838 1025.5530 1025.5546 -1.46 0 11 0.26 +1Score > 17 indicates identity IKPYTYNK
2422 567.2902 1132.5658 1132.5434 19.8 1 10 0.2 +1Score > 21 indicates identity
Score > 16 indicates homology		 KMDNPNGIVK + 2 Deamidated (NQ); Oxidation (M)
1824 487.2614 972.5082 972.5127 -4.62 0 10 0.25 +1Score > 21 indicates identity
Score > 17 indicates homology		 IENELEVK
1241 362.7235 723.4324 723.4279 6.28 0 10 0.095 +1Score > 13 indicates identity IPELPR
1918 500.2973 998.5800 998.5873 -7.21 1 10 0.31 +1Score > 18 indicates identity QNLDILKR
1467 845.4021 844.3948 844.4113 -19.5 0 10 0.31 +1Score > 17 indicates identity NEINPMK
1565 443.7686 885.5226 885.5072 17.4 0 10 0.62 +1Score > 20 indicates identity HGYGLVIK
2832 689.3381 1376.6616 1376.6605 0.81 1 10 0.16 +1Score > 22 indicates identity
Score > 14 indicates homology		 EGRNMTIADEIK + Deamidated (NQ)
983 551.2851 550.2778 550.2785 -1.18 1 10 0.16 +1Score > 20 indicates identity
Score > 14 indicates homology		 KSGML + Oxidation (M)
3853 1061.4880 2120.9614 2120.9862 -11.7 1 10 0.53 +1Score > 20 indicates identity IQEQNNSVEIYSDGNKGPK + 2 Deamidated (NQ)
2800 667.8411 1333.6676 1333.6812 -10.2 1 10 0.92 +1Score > 22 indicates identity IRFQEMGLAPR + Deamidated (NQ); Oxidation (M)
2250 1088.5419 1087.5346 1087.5338 0.73 0 10 0.72 +1Score > 21 indicates identity NEGFLAFYK
1051 306.6775 611.3404 611.3391 2.19 0 10 0.11 +1Score > 13 indicates identity AVTHGK
2920 724.3574 1446.7002 1446.6959 2.98 0 10 0.83 +1Score > 21 indicates identity LNGGLGTSMGCVGPK
1958 1009.5026 1008.4953 1008.4777 17.5 1 10 0.95 +1Score > 22 indicates identity HDSYKGFR
2303 1103.5101 1102.5028 1102.4825 18.4 0 9 0.44 +1Score > 18 indicates identity MSLNGSAHNR + Deamidated (NQ); Oxidation (M)
1102 644.3387 643.3314 643.3289 3.90 0 9 0.26 +1Score > 16 indicates identity GLNPSR + Deamidated (NQ)
989 558.2895 557.2822 557.2809 2.35 0 9 0.17 +1Score > 14 indicates identity QGEPK
1763 477.2205 952.4264 952.4138 13.3 0 9 0.47 +1Score > 18 indicates identity EGGGNNIYK + 2 Deamidated (NQ)
1153 338.1770 674.3394 674.3347 7.02 1 9 0.61 +1Score > 21 indicates identity
Score > 20 indicates homology		 KNNNGK + Deamidated (NQ)
2263 1090.5385 1089.5312 1089.5190 11.3 0 9 1 +1Score > 22 indicates identity
Score > 22 indicates homology		 ANVETQIQGK + 3 Deamidated (NQ)
2721 629.8011 1257.5876 1257.5990 -8.99 0 9 0.44 +1Score > 18 indicates identity YSSTFLSSNPR
1006 574.3289 573.3216 573.3196 3.51 0 9 1 +1Score > 23 indicates identity
Score > 22 indicates homology		 LPMIT
2583 390.8729 1169.5969 1169.6153 -15.7 0 9 0.72 +1Score > 20 indicates identity HNSISGSLSLR
1460 421.7382 841.4618 841.4657 -4.61 1 9 0.39 +1Score > 17 indicates identity QPINNKK + Deamidated (NQ)
2968 742.8788 1483.7430 1483.7592 -10.9 0 9 0.23 +1Score > 21 indicates identity
Score > 15 indicates homology		 MLISNITFSISNK + Deamidated (NQ); Oxidation (M)
1426 828.4449 827.4376 827.4501 -15.1 0 9 0.46 +1Score > 18 indicates identity TGTPSPLR
2599 588.2999 1174.5852 1174.5690 13.8 1 9 0.56 +1Score > 22 indicates identity
Score > 19 indicates homology		 NNSNNSIQRK + Deamidated (NQ)
2898 720.8370 1439.6594 1439.6602 -0.53 1 9 0.68 +1Score > 19 indicates identity LGFMEAASDDEKK
2256 545.2949 1088.5752 1088.5614 12.7 1 9 0.17 +1Score > 21 indicates identity
Score > 13 indicates homology		 RIADNFPEK
1320 386.2039 770.3932 770.3922 1.31 0 9 0.42 +1Score > 17 indicates identity NANQVPK + Deamidated (NQ)
2938 486.8908 1457.6506 1457.6786 -19.3 0 9 0.52 +1Score > 18 indicates identity EIDWNSNNEPIK
1718 470.2388 938.4630 938.4644 -1.41 1 8 0.71 +1Score > 20 indicates identity KVSGNFMR + Deamidated (NQ)
2681 608.7825 1215.5504 1215.5666 -13.3 1 8 0.44 +1Score > 17 indicates identity MGQGASSHGQKK + Deamidated (NQ)
1064 618.3315 617.3242 617.3173 11.2 1 8 0.17 +1Score > 23 indicates identity
Score > 13 indicates homology		 KDIGW
2249 544.7735 1087.5324 1087.5397 -6.66 0 8 1 +1Score > 21 indicates identity
Score > 21 indicates homology		 IGELNDEVAK + Deamidated (NQ)
2104 1048.5491 1047.5418 1047.5349 6.61 0 8 1 +1Score > 22 indicates identity
Score > 21 indicates homology		 GAVASASFNPK
1611 906.4334 905.4261 905.4429 -18.5 1 8 1 +1Score > 21 indicates identity
Score > 21 indicates homology		 KDHMFTK
1208 351.1836 700.3526 700.3504 3.25 0 8 0.33 +1Score > 16 indicates identity LNNPSR + Deamidated (NQ)
1537 878.4486 877.4413 877.4294 13.6 0 8 0.82 +1Score > 22 indicates identity
Score > 20 indicates homology		 LSSFNGPR + Deamidated (NQ)
1063 309.6666 617.3186 617.3173 2.17 1 8 0.18 +1Score > 22 indicates identity
Score > 13 indicates homology		 KDIGW
2387 1126.5139 1125.5066 1125.4907 14.2 1 8 0.54 +1Score > 18 indicates identity MGKVMSNNGR + Deamidated (NQ); 2 Oxidation (M)
1470 423.2317 844.4488 844.4555 -7.92 0 8 0.55 +1Score > 23 indicates identity
Score > 18 indicates homology		 NLGTVWR
2598 588.2881 1174.5616 1174.5578 3.28 1 8 0.51 +1Score > 21 indicates identity
Score > 17 indicates homology		 VETDNRANEK
1658 461.7556 921.4966 921.4807 17.3 0 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 VSYQSLPK + Deamidated (NQ)
2652 596.8052 1191.5958 1191.5772 15.7 0 8 0.53 +1Score > 22 indicates identity
Score > 18 indicates homology		 SAPVLNDSFDK
2209 538.7756 1075.5366 1075.5484 -11.0 1 8 0.69 +1Score > 21 indicates identity
Score > 19 indicates homology		 MDIPPGFKR + Oxidation (M)
3429 611.9625 1832.8657 1832.8985 -17.9 1 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 CIINQSQNSQRMLNK
1061 616.3698 615.3625 615.3592 5.47 0 8 1 +1Score > 25 indicates identity
Score > 20 indicates homology		 AVLEGK
1441 418.2383 834.4620 834.4599 2.52 1 8 0.91 +1Score > 20 indicates identity KTPQSFK
1762 953.4335 952.4262 952.4138 13.1 0 8 0.7 +1Score > 19 indicates identity EGGGNNIYK + 2 Deamidated (NQ)
1432 833.3923 832.3850 832.3927 -9.17 0 8 0.91 +1Score > 20 indicates identity GIDQASDK
1386 807.4343 806.4270 806.4174 11.9 0 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 ADTYIPK
1754 475.7401 949.4656 949.4730 -7.70 0 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 HNHSTLNK
1601 903.5104 902.5031 902.5073 -4.60 0 8 0.25 +1Score > 22 indicates identity
Score > 14 indicates homology		 VLQSALSGK + Deamidated (NQ)
1469 423.2231 844.4316 844.4232 10.0 0 7 0.21 +1Score > 21 indicates identity
Score > 13 indicates homology		 WDFHIK
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