MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:28:59 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 22)

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+1

Accession Score Description
1 W0T3V3_KLUMD 117 Enoate reductase 1 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=KYE1 PE=4 SV=1

+2

Accession Score Description
1 W0T3H5_KLUMD 98 Phosphoglycerate kinase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=PGK PE=3 SV=1

-3

Accession Score Description
1 W0T7K9_KLUMD 85 phosphopyruvate hydratase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=ENO PE=3 SV=1
Score Mass Matches Sequences emPAI
3.1 W0T7K9_KLUMD 85 46830 6 (5) 4 (3) 0.31
phosphopyruvate hydratase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=ENO PE=3 SV=1

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1245   726.3441 725.3368 725.3344 3.33 0 2 1.9 +1Score > 18 indicates identity U R.SVYDSR.G
1371   800.3859 799.3786 799.3752 4.27 0 28 0.003 +1Score > 16 indicates identity U K.YDLDFK.N
1372 +1 400.6966 799.3786 799.3752 4.30 0 28 0.0032 +1Score > 16 indicates identity U K.YDLDFK.N
1603   452.7356 903.4566 903.4549 1.94 0 45 0.00031 +1Score > 22 indicates identity U R.IEEELGSK.A
1819   486.7506 971.4866 971.4825 4.32 1 32 0.0026 +1Score > 19 indicates identity U K.KFHNANQL.- + Deamidated (NQ)

+4

Accession Score Description
1 W0TKF9_KLUMD 72 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=MET6 PE=3 SV=1

+5

Accession Score Description
1 W0TBW7_KLUMD 72 inositol-3-phosphate synthase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=INO1 PE=3 SV=1

+6

Accession Score Description
1 W0T5M8_KLUMD 46 Heat shock protein SSA2 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=SSA2 PE=3 SV=1

+7

Accession Score Description
1 W0TFH4_KLUMD 32 Acyl-CoA-binding protein,ACBP OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=ACB1 PE=4 SV=2

+8

Accession Score Description
1 W0TG93_KLUMD 32 Glyceraldehyde-3-phosphate dehydrogenase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=GAP3 PE=3 SV=1

+9

Accession Score Description
1 W0TJ61_KLUMD 26 Heterogeneous nuclear rnp K-like protein 2 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=HEK2 PE=3 SV=1

+10

Accession Score Description
1 W0T4R5_KLUMD 26 Glucose-6-phosphate isomerase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=RAG2 PE=3 SV=1
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