MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1245_Other_P1_B9.mgf
Database : K-marxianus 20241107 (5,052 sequences; 2,515,027 residues)
Timestamp : 24 Jun 2025 at 21:28:59 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,728

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 22)

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-11

Accession Score Description
1 W0TCK0_KLUMD 25 Vacuolar protein sorting-associated protein 70 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=VPS70 PE=3 SV=1
Score Mass Matches Sequences emPAI
11.1 W0TCK0_KLUMD 25 87327 3 (2) 2 (1) 0.04
Vacuolar protein sorting-associated protein 70 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=VPS70 PE=3 SV=1

-3 peptide matches (2 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1420 +1 413.7583 825.5020 825.4960 7.34 0 21 0.011 +1Score > 14 indicates identity U R.EVQPILK.K
3505   952.9805 1903.9464 1903.9778 -16.5 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 U K.NLIAYLNVDVAVDGGQLK.I + 3 Deamidated (NQ)

+12

Accession Score Description
1 W0TA84_KLUMD 24 Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=VIP1 PE=3 SV=1

+13

Accession Score Description
1 W0TGU0_KLUMD 24 Protein UTH1 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=UTH1 PE=3 SV=1

+14

Accession Score Description
1 W0TJX1_KLUMD 24 HTH APSES-type domain-containing protein OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=XBP1 PE=4 SV=1

+15

Accession Score Description
1 W0T9G9_KLUMD 24 Aminopeptidase OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=APE2 PE=3 SV=1

+16

Accession Score Description
1 W0TC04_KLUMD 21 Uncharacterized protein YJL016W/YJL017W OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=KLMA_40282 PE=4 SV=1

+17

Accession Score Description
1 W0TAM7_KLUMD 21 DNA-directed RNA polymerase subunit beta OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=RET1 PE=3 SV=1

+18

Accession Score Description
1 W0T9D7_KLUMD 21 Vacuolar protein sorting-associated protein 41 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=VPS41 PE=3 SV=1

+19

Accession Score Description
1 W0TCN4_KLUMD 21 tRNA pseudouridine synthase 1 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=PUS1 PE=3 SV=1

+20

Accession Score Description
1 W0TJC8_KLUMD 20 Protein DSE1 OS=Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) OX=1003335 GN=DSE1 PE=4 SV=1
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