MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
127 313.1841 312.1768
128 313.1850 312.1777
129 313.1853 312.1780
130 313.1862 312.1789
131 313.1862 312.1789
132 313.1865 312.1792
133 313.2216 312.2143
134 313.2222 312.2149
135 313.2388 312.2315
136 313.2401 312.2328
137 315.2013 314.1940
138 315.2020 314.1947
139 316.1911 315.1838
140 317.2108 316.2035
141 318.1302 317.1229
142 319.1898 318.1825
143 319.1904 318.1831
144 319.1904 318.1831
145 319.1904 318.1831
146 319.1908 318.1835
147 319.1909 318.1836
148 319.1915 318.1842
149 319.1917 318.1844
150 319.2266 318.2193
151 319.2271 318.2198
152 319.2271 318.2198
153 319.2272 318.2199
154 319.2272 318.2199
155 319.2275 318.2202
156 319.2277 318.2204
157 321.1691 320.1618
158 321.1695 320.1622
159 321.1697 320.1624
160 321.1701 320.1628
161 321.1703 320.1630
162 321.1706 320.1633
163 321.1711 320.1638
164 321.1712 320.1639
165 321.2049 320.1976
166 321.2059 320.1986
167 321.2060 320.1987
168 321.2061 320.1988
169 321.2430 320.2357
170 321.2619 320.2546
171 322.2451 321.2378
172 323.1681 322.1608
173 323.1684 322.1611
174 323.1688 322.1615
175 323.1691 322.1618
176 323.1693 322.1620
177 323.1700 322.1627
178 323.1857 322.1784
179 323.1858 322.1785
180 323.1858 322.1785
181 323.1858 322.1785
182 323.1860 322.1787
183 323.1862 322.1789
184 323.1866 322.1793
185 323.1870 322.1797
186 323.1873 322.1800
187 323.1876 322.1803
188 323.1878 322.1805
189 323.2032 322.1959
190 323.2054 322.1981
191 323.2061 322.1988
192 324.1718 323.1645
193 325.1823 324.1750
194 325.1833 324.1760
195 325.1835 324.1762
196 325.1835 324.1762
197 325.1838 324.1765
198 325.1839 324.1766
199 325.1846 324.1773
200 325.1849 324.1776
201 325.1853 324.1780
202 325.1869 324.1796
203 325.2019 324.1946
204 325.2027 324.1954
205 325.2031 324.1958
206 325.2211 324.2138
207 325.2220 324.2147
208 325.2935 324.2862
209 326.1869 325.1796
210 327.1329 326.1256
211 327.1330 326.1257
212 327.1333 326.1260
213 327.1633 326.1560
214 327.1637 326.1564
215 327.2010 326.1937
216 327.2013 326.1940
217 327.2015 326.1942
218 327.2017 326.1944
219 327.2018 326.1945
220 327.2038 326.1965
221 327.2175 326.2102
222 327.2183 326.2110
223 327.2184 326.2111
224 329.1782 328.1709
225 329.1782 328.1709
226 329.1782 328.1709
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