MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
27 305.1760 304.1687
28 305.1949 304.1876
29 305.2106 304.2033
30 307.1534 306.1461
31 307.1536 306.1463
32 307.1543 306.1470
33 307.1547 306.1474
34 307.1549 306.1476
35 307.1896 306.1823
36 307.1907 306.1834
37 307.1908 306.1835
38 307.1909 306.1836
39 307.1910 306.1837
40 307.1911 306.1838
41 307.1916 306.1843
42 307.1919 306.1846
43 307.1920 306.1847
44 307.1921 306.1848
45 307.1922 306.1849
46 307.2094 306.2021
47 308.0891 307.0818
48 308.0896 307.0823
49 308.0897 307.0824
50 308.0897 307.0824
51 308.0898 307.0825
52 308.0900 307.0827
53 308.0900 307.0827
54 308.0901 307.0828
55 308.0901 307.0828
56 308.0903 307.0830
57 308.0903 307.0830
58 308.0904 307.0831
59 308.0907 307.0834
60 308.0909 307.0836
61 308.0912 307.0839
62 308.0919 307.0846
63 308.0919 307.0846
64 308.0921 307.0848
65 308.0921 307.0848
66 308.0922 307.0849
67 308.0923 307.0850
68 308.0923 307.0850
69 308.0926 307.0853
70 308.0926 307.0853
71 308.0926 307.0853
72 308.0927 307.0854
73 308.0927 307.0854
74 308.0927 307.0854
75 308.0928 307.0855
76 308.0929 307.0856
77 308.0929 307.0856
78 308.0930 307.0857
79 308.0931 307.0858
80 309.1530 308.1457
81 309.1701 308.1628
82 309.1703 308.1630
83 309.1703 308.1630
84 309.1704 308.1631
85 309.1711 308.1638
86 309.1882 308.1809
87 309.1890 308.1817
88 309.1891 308.1818
89 309.1895 308.1822
90 309.1901 308.1828
91 309.2054 308.1981
92 309.2071 308.1998
93 309.2074 308.2001
94 309.2077 308.2004
95 309.2080 308.2007
96 309.2084 308.2011
97 309.2089 308.2016
98 310.1915 309.1842
99 310.1918 309.1845
100 311.1676 310.1603
101 311.1677 310.1604
102 311.1679 310.1606
103 311.1684 310.1611
104 311.1690 310.1617
105 311.1694 310.1621
106 311.1701 310.1628
107 311.1850 310.1777
108 311.1853 310.1780
109 311.1855 310.1782
110 311.2052 310.1979
111 311.2059 310.1986
112 311.2061 310.1988
113 311.2065 310.1992
114 311.2079 310.2006
115 311.2238 310.2165
116 311.2238 310.2165
117 311.2245 310.2172
118 311.2246 310.2173
119 312.1328 311.1255
120 312.1328 311.1255
121 312.1331 311.1258
122 312.1332 311.1259
123 313.1222 312.1149
124 313.1831 312.1758
125 313.1840 312.1767
126 313.1841 312.1768
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