MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 4 |
| MS data file | : | PRT1245_Other_P1_B12.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:22:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,772 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 2525 |
606.7896 | 1211.5646 | 1211.5822 | -14.5 | 1 | 0 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
KDSIYANWSK + Deamidated (NQ) |
| 2844 |
749.3752 | 1496.7358 | 1496.7147 | 14.1 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
LDSEFQPLSSAFR + Deamidated (NQ) |
| 3022 |
534.9493 | 1601.8261 | 1601.8382 | -7.55 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
STVPIIIRCCIDR |
| 2252 |
567.2847 | 1132.5548 | 1132.5335 | 18.8 | 0 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
MEAHNQFLK + Oxidation (M) |
| 2602 |
643.8290 | 1285.6434 | 1285.6374 | 4.68 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
INGNGKNNIDAR + Deamidated (NQ) |
| 3681 |
684.9840 | 2051.9302 | 2051.9436 | -6.55 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
EAVVDDGSENAFGIPEFTR |
| 1345 |
874.4278 | 873.4205 | 873.4192 | 1.55 | 0 | 0 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
NNQIEEK |
| 1616 |
483.7361 | 965.4576 | 965.4753 | -18.3 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
IATRGAFAC |
| 2561 |
625.3345 | 1248.6544 | 1248.6350 | 15.6 | 1 | 0 | 0.94 | 1Score > 22 indicates identity Score > 13 indicates homology |
ELNLGKDEFGK |
| 1299 |
854.4123 | 853.4050 | 853.4181 | -15.4 | 0 | 0 | 3.3 | 1Score > 18 indicates identity |
VATGYSEK |
| 3506 |
976.4488 | 1950.8830 | 1950.9026 | -10.0 | 1 | 0 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
LTASPDNLASMMSKDQNK + Deamidated (NQ) |
| 1873 |
520.7551 | 1039.4956 | 1039.5008 | -4.96 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
NEGIMGLYK + Oxidation (M) |
| 3234 |
584.2688 | 1749.7846 | 1749.7614 | 13.2 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
ANINDLNEVLDQCTK + 4 Deamidated (NQ) |
| 1334 |
867.4229 | 866.4156 | 866.4021 | 15.6 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
INSEQFK + 2 Deamidated (NQ) |
| 2763 |
723.8716 | 1445.7286 | 1445.7085 | 13.9 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
SHGAVLPSYCSLR |
| 1615 |
483.7354 | 965.4562 | 965.4753 | -19.7 | 1 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
IATRGAFAC |
| 2256 |
567.7828 | 1133.5510 | 1133.5313 | 17.5 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
QNTSQNNSIK + Deamidated (NQ) |
| 2872 |
757.9145 | 1513.8144 | 1513.8075 | 4.60 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
KPTMIKLQFHNR + 2 Deamidated (NQ) |
| 3047 |
810.9093 | 1619.8040 | 1619.8042 | -0.11 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
ALDQSISKLQSWDK + 2 Deamidated (NQ) |
| 3801 |
701.3392 | 2100.9958 | 2101.0368 | -19.5 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
STFREQIIYPDSIEQFK + Deamidated (NQ) |
| 4388 |
920.4587 | 2758.3543 | 2758.3450 | 3.37 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
VTDFQFIQDTTKSLASVYLHSSNR + 2 Deamidated (NQ) |
| 2726 |
710.8868 | 1419.7590 | 1419.7544 | 3.27 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
NLAVVQTPMFRK + Deamidated (NQ); Oxidation (M) |
| 895 |
579.3149 | 578.3076 | 578.3098 | -3.71 | 0 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
AMITK + Oxidation (M) |
| 1106 |
754.3371 | 753.3298 | 753.3268 | 3.98 | 0 | 0 | 2.4 | 1Score > 17 indicates identity |
NWFCK |
| 2203 |
1119.6149 | 1118.6076 | 1118.6118 | -3.71 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
IMVIGGKNSGK + Oxidation (M) |
| 2853 |
752.8713 | 1503.7280 | 1503.7425 | -9.64 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
SPTGIVLMNMGGPSK + Oxidation (M) |
| 3097 |
830.4290 | 1658.8434 | 1658.8416 | 1.10 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
DWIQNGRPNLFGLK + 2 Deamidated (NQ) |
| 3992 |
735.3275 | 2202.9607 | 2202.9813 | -9.38 | 0 | 0 | 3.4 | 1Score > 18 indicates identity |
GANIASFVMVADAMLDQGDVF + Deamidated (NQ); 2 Oxidation (M) |
| 2494 |
596.8206 | 1191.6266 | 1191.6288 | -1.83 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
GVLFYGPPGTGK |
| 976 |
328.1745 | 654.3344 | 654.3449 | -16.0 | 0 | 0 | 2.8 | 1Score > 17 indicates identity |
LTQHR + Deamidated (NQ) |
| 1085 |
742.3368 | 741.3295 | 741.3333 | -5.15 | 0 | 0 | 2.4 | 1Score > 17 indicates identity |
GEFFNK + Deamidated (NQ) |
| 1164 |
783.3701 | 782.3628 | 782.3745 | -14.9 | 0 | 0 | 3.8 | 1Score > 19 indicates identity |
MSNFIR + Oxidation (M) |
| 2154 |
553.2949 | 1104.5752 | 1104.5788 | -3.23 | 1 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
QRSFNANLR |
| 2250 |
1133.5479 | 1132.5406 | 1132.5183 | 19.8 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
MSAPEAQQQK + Oxidation (M) |
| 2660 |
672.8266 | 1343.6386 | 1343.6510 | -9.18 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
DGEWLYHISPK |
| 2950 |
521.6019 | 1561.7839 | 1561.7997 | -10.1 | 0 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
VLQTMSFLMNHLI + Oxidation (M) |
| 3182 |
574.9203 | 1721.7391 | 1721.7340 | 2.96 | 1 | 0 | 3.3 | 1Score > 18 indicates identity |
STPQDTNVNSQSRER + 4 Deamidated (NQ) |
| 2734 |
715.8345 | 1429.6544 | 1429.6547 | -0.19 | 0 | 0 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
NYVMQYIQENK + Deamidated (NQ) |
| 4393 |
922.4741 | 2764.4005 | 2764.3483 | 18.9 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
IKSFGDSVPYIEEALQVYSTEFDK |
| 2072 |
544.7736 | 1087.5326 | 1087.5397 | -6.46 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
LNGQLNLEGK + 3 Deamidated (NQ) |
| 4135 |
775.7155 | 2324.1247 | 2324.1610 | -15.6 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
TVAGFSNVELPQCIIPSSYIK + 2 Deamidated (NQ) |
| 2021 |
1077.5281 | 1076.5208 | 1076.5210 | -0.17 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
NSSQRSQLR + 2 Deamidated (NQ) |
| 2783 |
728.3552 | 1454.6958 | 1454.7041 | -5.69 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
GINELLGFNSSFR + 2 Deamidated (NQ) |
| 3417 |
937.4588 | 1872.9030 | 1872.9073 | -2.28 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
LINRTNCPTQIMNPAK + 3 Deamidated (NQ) |
| 2951 |
521.6026 | 1561.7860 | 1561.7997 | -8.76 | 0 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
VLQTMSFLMNHLI + Oxidation (M) |
| 2150 |
553.2875 | 1104.5604 | 1104.5597 | 0.67 | 0 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
LLNNLSMER + Oxidation (M) |
| 1035 |
347.1678 | 692.3210 | 692.3275 | -9.38 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
MKQGGR + Deamidated (NQ); Oxidation (M) |
| 2685 |
687.3422 | 1372.6698 | 1372.6582 | 8.46 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
NIEASVQPSRDR + 2 Deamidated (NQ) |
| 3203 |
868.4240 | 1734.8334 | 1734.8617 | -16.3 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
ERNYIVAFIFGNYK + 2 Deamidated (NQ) |
| 2874 |
758.8688 | 1515.7230 | 1515.7279 | -3.20 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
APEIMLSYQGYTK + Oxidation (M) |
| 3115 |
836.9404 | 1671.8662 | 1671.8355 | 18.4 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
EAVHILLDEIDDYK |
| 3149 |
847.3816 | 1692.7486 | 1692.7559 | -4.31 | 1 | 0 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
ETSNKQTACPICQR + Deamidated (NQ) |
| 3680 |
1026.5051 | 2050.9956 | 2051.0058 | -4.96 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
NQYGLLKQTQELEETQK + 2 Deamidated (NQ) |
| 1754 |
504.2505 | 1006.4864 | 1006.4832 | 3.23 | 1 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
NGGKGGENFK |
| 2466 |
591.8113 | 1181.6080 | 1181.5863 | 18.4 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
MYIQLEGRR + Deamidated (NQ); Oxidation (M) |
| 3963 |
1091.9952 | 2181.9758 | 2181.9437 | 14.7 | 1 | 0 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
ENDIFELKDVDDQNQTQK + 4 Deamidated (NQ) |
| 1473 |
461.7551 | 921.4956 | 921.4920 | 4.00 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
NSFSNIIK |
| 2095 |
547.7654 | 1093.5162 | 1093.5186 | -2.14 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
SQVSQNRMK + Deamidated (NQ); Oxidation (M) |
| 4259 |
821.3983 | 2461.1731 | 2461.1319 | 16.7 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
SIQDLTNSMLQFSDPLTTYNR + 2 Deamidated (NQ); Oxidation (M) |
| 1445 |
456.2336 | 910.4526 | 910.4509 | 1.97 | 0 | 0 | 4.9 | 1Score > 20 indicates identity |
GQNPVDGPK |
| 3221 |
872.4072 | 1742.7998 | 1742.7999 | -0.028 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
FSVNSEEDLNFSEVK |
| 1151 |
778.3754 | 777.3681 | 777.3552 | 16.7 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
GQGGMRR + Deamidated (NQ); Oxidation (M) |
| 2183 |
557.7585 | 1113.5024 | 1113.5012 | 1.12 | 1 | 0 | 4.3 | 1Score > 19 indicates identity |
YIDDGMQKK + Deamidated (NQ); Oxidation (M) |
| 4277 |
832.4216 | 2494.2430 | 2494.2486 | -2.24 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
NEVTCSITGDNPIHKINNGLGLK + Deamidated (NQ) |
| 2467 |
591.8118 | 1181.6090 | 1181.5863 | 19.3 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
MYIQLEGRR + Deamidated (NQ); Oxidation (M) |
| 4138 |
777.3703 | 2329.0891 | 2329.1260 | -15.8 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
MSDNLLSLENPVVPSHYELR + Deamidated (NQ); Oxidation (M) |
| 4369 |
904.4234 | 2710.2484 | 2710.2288 | 7.23 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
EDAMIAQESMNNYLLMGHLLQVR + 3 Deamidated (NQ); 2 Oxidation (M) |
| 1170 |
784.3896 | 783.3823 | 783.3949 | -16.0 | 1 | 0 | 3 | 1Score > 17 indicates identity |
CKYVSK |
| 4004 |
739.3283 | 2214.9631 | 2214.9742 | -5.00 | 1 | 0 | 3.8 | 1Score > 18 indicates identity |
VQLCTLMNIKSGGCSEDCK + Oxidation (M) |
| 2583 |
636.3024 | 1270.5902 | 1270.5789 | 8.93 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
NGQASAPRPNQK + 4 Deamidated (NQ) |
| 2440 |
1176.5566 | 1175.5493 | 1175.5459 | 2.94 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
GVEAGDFEQPK |
| 2549 |
618.7977 | 1235.5808 | 1235.5703 | 8.53 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
NAAMEVQEAKK + 2 Deamidated (NQ); Oxidation (M) |
| 2562 |
625.3350 | 1248.6554 | 1248.6350 | 16.4 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
ELNLGKDEFGK |
| 3235 |
875.9709 | 1749.9272 | 1749.9108 | 9.38 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
SLLTSQLSSSTEKVNR + Deamidated (NQ) |
| 1 |
300.2086 | 299.2013 | |||||||
| 2 |
301.1434 | 300.1361 | |||||||
| 3 |
301.1436 | 300.1363 | |||||||
| 4 |
301.1436 | 300.1363 | |||||||
| 5 |
301.1437 | 300.1364 | |||||||
| 6 |
301.1439 | 300.1366 | |||||||
| 7 |
301.1440 | 300.1367 | |||||||
| 8 |
301.1444 | 300.1371 | |||||||
| 9 |
301.2210 | 300.2137 | |||||||
| 10 |
303.8863 | 302.8790 | |||||||
| 11 |
303.8866 | 302.8793 | |||||||
| 12 |
303.8870 | 302.8797 | |||||||
| 13 |
303.8872 | 302.8799 | |||||||
| 14 |
303.8873 | 302.8800 | |||||||
| 15 |
305.0122 | 304.0049 | |||||||
| 16 |
305.1735 | 304.1662 | |||||||
| 17 |
305.1745 | 304.1672 | |||||||
| 18 |
305.1748 | 304.1675 | |||||||
| 19 |
305.1750 | 304.1677 | |||||||
| 20 |
305.1750 | 304.1677 | |||||||
| 21 |
305.1752 | 304.1679 | |||||||
| 22 |
305.1754 | 304.1681 | |||||||
| 23 |
305.1756 | 304.1683 | |||||||
| 24 |
305.1758 | 304.1685 | |||||||
| 25 |
305.1759 | 304.1686 | |||||||
| 26 |
305.1760 | 304.1687 | |||||||
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