| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 4 |
| MS data file | : | PRT1245_Other_P1_B12.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:22:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,772 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 845.9479 | 1689.8812 | 1689.8542 | 16.0 | 1 | 1 | 0.84 | 1Score > 21 indicates identityScore > 13 indicates homology |
KIICPSVESISNGMR | |
| 970.9824 | 1939.9502 | 1939.9231 | 14.0 | 0 | 1 | 1 | 1Score > 22 indicates identityScore > 14 indicates homology |
GISNMMLSLTIENSEGTK + Oxidation (M) | |
| 456.2334 | 910.4522 | 910.4508 | 1.55 | 0 | 1 | 3.9 | 1Score > 20 indicates identity |
DLEVNGHK | |
| 756.8346 | 1511.6546 | 1511.6762 | -14.2 | 0 | 1 | 2.8 | 1Score > 18 indicates identity |
TAAAMWGCHIHNK + Oxidation (M) | |
| 523.9294 | 1568.7664 | 1568.7617 | 3.00 | 0 | 1 | 0.88 | 1Score > 21 indicates identityScore > 13 indicates homology |
MNNTSITGPQVLHR + 2 Deamidated (NQ) | |
| 1081.5217 | 2161.0288 | 2161.0547 | -12.0 | 0 | 1 | 1 | 1Score > 22 indicates identityScore > 14 indicates homology |
MGPTFGAMALSGVHAAEQILK + Deamidated (NQ); 2 Oxidation (M) | |
| 1152.5762 | 2303.1378 | 2303.1501 | -5.31 | 1 | 1 | 0.96 | 1Score > 22 indicates identityScore > 13 indicates homology |
LQQLSESIKITEMQLNHMK + Deamidated (NQ); 2 Oxidation (M) | |
| 934.4514 | 933.4441 | 933.4376 | 6.97 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 14 indicates homology |
NNSRSNSR | |
| 601.7903 | 1201.5660 | 1201.5761 | -8.36 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 14 indicates homology |
ANTPSSMHKTK + Deamidated (NQ) | |
| 342.1962 | 682.3778 | 682.3762 | 2.41 | 1 | 1 | 2.3 | 1Score > 17 indicates identity |
GPKEPR | |
| 953.4333 | 952.4260 | 952.4362 | -10.7 | 1 | 1 | 3.3 | 1Score > 19 indicates identity |
DAYRGQSR + Deamidated (NQ) | |
| 542.7632 | 1083.5118 | 1083.5019 | 9.22 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 14 indicates homology |
RLQGNGMYK + 2 Deamidated (NQ); Oxidation (M) | |
| 897.4385 | 896.4312 | 896.4426 | -12.7 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 14 indicates homology |
MSFVQIR + Deamidated (NQ); Oxidation (M) | |
| 469.9758 | 1875.8741 | 1875.9115 | -20.0 | 1 | 1 | 0.86 | 1Score > 22 indicates identityScore > 13 indicates homology |
REPFFHGTSNTDQLVK + Deamidated (NQ) | |
| 984.9896 | 1967.9646 | 1967.9297 | 17.8 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 14 indicates homology |
TSGLLAPTDDHNGEQTRR + Deamidated (NQ) | |
| 571.7576 | 1141.5006 | 1141.5000 | 0.59 | 0 | 1 | 2.8 | 1Score > 18 indicates identity |
GEPSEPQQDR | |
| 947.5040 | 1892.9934 | 1892.9672 | 13.8 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 14 indicates homology |
WIFESVWAGLTELTNK | |
| 722.3496 | 2164.0270 | 2164.0396 | -5.84 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 14 indicates homology |
SNNKNEDTFQQVSLNDIAK | |
| 843.0583 | 2526.1531 | 2526.1413 | 4.64 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 14 indicates homology |
FMYDTFDDHYQATIGIDFLSK | |
| 882.4701 | 881.4628 | 881.4607 | 2.45 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 14 indicates homology |
ANGGEPLPK | |
| 1068.5294 | 2135.0442 | 2135.0429 | 0.62 | 0 | 1 | 0.88 | 1Score > 23 indicates identityScore > 13 indicates homology |
LGIEIDNGNHMLLSGNHSAK + Oxidation (M) | |
| 932.7827 | 2795.3263 | 2795.3470 | -7.40 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 14 indicates homology |
NVIPLASKMEVGSNEDVSSMGNFINK + Oxidation (M) | |
| 794.9075 | 1587.8004 | 1587.8256 | -15.9 | 1 | 1 | 1 | 1Score > 24 indicates identityScore > 14 indicates homology |
AAQNPLADIQVYKR + 2 Deamidated (NQ) | |
| 930.1601 | 5574.9169 | 5574.8558 | 11.0 | 1 | 1 | 0.79 | 1Score > 13 indicates identity |
MLQFNTENDTVAPVFPMEQDINAAPDAVPLVQTTTLQVFVKLAEPIVFLK + Deamidated (NQ); 2 Oxidation (M) | |
| 372.6861 | 743.3576 | 743.3490 | 11.6 | 0 | 1 | 3.3 | 1Score > 19 indicates identity |
EGTYFK | |
| 1170.0486 | 2338.0826 | 2338.0522 | 13.0 | 0 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
LDFESQDVSNLDSNLGMDPLK + 2 Deamidated (NQ) | |
| 478.7625 | 955.5104 | 955.5127 | -2.35 | 0 | 1 | 2.9 | 1Score > 18 indicates identity |
NVPIWAQK + Deamidated (NQ) | |
| 836.4387 | 835.4314 | 835.4188 | 15.1 | 0 | 1 | 0.93 | 1Score > 23 indicates identityScore > 13 indicates homology |
ALNFTNR + Deamidated (NQ) | |
| 553.2915 | 1104.5684 | 1104.5788 | -9.38 | 1 | 1 | 0.82 | 1Score > 23 indicates identityScore > 13 indicates homology |
QRSFNANLR | |
| 695.3430 | 1388.6714 | 1388.6531 | 13.2 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
NPSRSIADSAQNK + 2 Deamidated (NQ) | |
| 933.1144 | 2796.3214 | 2796.3011 | 7.25 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
QLKFLNMENETTVDDLQDQLQEK + 2 Deamidated (NQ); Oxidation (M) | |
| 781.8945 | 1561.7744 | 1561.7592 | 9.74 | 0 | 1 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
IICPSVESISNGMR | |
| 578.2725 | 1154.5304 | 1154.5105 | 17.3 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
HNLADQDWR + Deamidated (NQ) | |
| 669.3469 | 1336.6792 | 1336.6834 | -3.10 | 1 | 1 | 0.99 | 1Score > 23 indicates identityScore > 13 indicates homology |
KESGLTSNSLSSK | |
| 468.7132 | 935.4118 | 935.4270 | -16.2 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
TSEMLDPK + Oxidation (M) | |
| 475.2597 | 948.5048 | 948.4950 | 10.4 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
AMKNIEVK + Deamidated (NQ); Oxidation (M) | |
| 560.7792 | 1119.5438 | 1119.5349 | 8.00 | 0 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
NILFFNHNT + Deamidated (NQ) | |
| 1148.5342 | 1147.5269 | 1147.5217 | 4.53 | 1 | 1 | 0.83 | 1Score > 21 indicates identityScore > 13 indicates homology |
SKNSNVNNNR + 2 Deamidated (NQ) | |
| 1068.5321 | 2135.0496 | 2135.0146 | 16.4 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
IQVHYMNYGESIKFFSR + Deamidated (NQ); Oxidation (M) | |
| 707.8364 | 1413.6582 | 1413.6785 | -14.3 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
DWMKMVANLYK + Oxidation (M) | |
| 636.3036 | 1270.5926 | 1270.5830 | 7.62 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
DADFRYQDLK + Deamidated (NQ) | |
| 898.0777 | 2691.2113 | 2691.2295 | -6.78 | 1 | 1 | 4.1 | 1Score > 19 indicates identity |
CYKQLPLGEVAQQQYDSMITSTK + 4 Deamidated (NQ) | |
| 939.4550 | 1876.8954 | 1876.8634 | 17.1 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
CQYFVTLLCMERNK + Oxidation (M) | |
| 657.3573 | 656.3500 | 656.3493 | 1.07 | 0 | 1 | 2 | 1Score > 16 indicates identity |
LGENPK | |
| 496.2668 | 990.5190 | 990.5056 | 13.6 | 0 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
AMGINDLLK + Deamidated (NQ); Oxidation (M) | |
| 462.5398 | 1384.5976 | 1384.5929 | 3.41 | 1 | 1 | 3 | 1Score > 18 indicates identity |
YNNENGSMPSKK + Deamidated (NQ); Oxidation (M) | |
| 451.8979 | 1352.6719 | 1352.6659 | 4.39 | 1 | 1 | 0.86 | 1Score > 22 indicates identityScore > 13 indicates homology |
MASRFTFPPQR + Oxidation (M) | |
| 834.4146 | 2500.2220 | 2500.2341 | -4.87 | 0 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
APQAAGVIHNDLMNTFILAQVMK + 3 Deamidated (NQ); Oxidation (M) | |
| 821.8687 | 1641.7228 | 1641.6907 | 19.6 | 0 | 1 | 4.1 | 1Score > 19 indicates identity |
FYNTHTGSNINDTR + 3 Deamidated (NQ) | |
| 916.9874 | 1831.9602 | 1831.9325 | 15.2 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
VTKLDVPMGIMHFNSK + Oxidation (M) | |
| 715.3929 | 2143.1569 | 2143.1623 | -2.55 | 1 | 1 | 2.9 | 1Score > 18 indicates identity |
SSDITAILPTEQSQKLVLAK + 2 Deamidated (NQ) | |
| 860.4171 | 859.4098 | 859.4188 | -10.4 | 0 | 1 | 3.1 | 1Score > 18 indicates identity |
WADEALR | |
| 938.4500 | 1874.8854 | 1874.8799 | 2.97 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
YDNPTGGVYQVYNTRK + Deamidated (NQ) | |
| 589.7953 | 1177.5760 | 1177.5649 | 9.51 | 1 | 1 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
QLSEMQKDAK + Deamidated (NQ) | |
| 1039.4954 | 3115.4644 | 3115.5230 | -18.8 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
LENDLNEELVGQLSKTMDNLENLTIPR + 2 Deamidated (NQ); Oxidation (M) | |
| 1124.5410 | 1123.5337 | 1123.5397 | -5.29 | 0 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
SLQNYLQEK + 2 Deamidated (NQ) | |
| 498.7416 | 995.4686 | 995.4560 | 12.7 | 0 | 1 | 0.99 | 1Score > 21 indicates identityScore > 13 indicates homology |
QGLEGNSYK + Deamidated (NQ) | |
| 781.8972 | 1561.7798 | 1561.8100 | -19.3 | 1 | 1 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
DINDININFAAKSK | |
| 778.3740 | 777.3667 | 777.3691 | -3.01 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
KLDNCK + Deamidated (NQ) | |
| 816.9005 | 1631.7864 | 1631.7712 | 9.35 | 1 | 1 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
LGQQNNLPKLDMNK + 4 Deamidated (NQ); Oxidation (M) | |
| 947.4977 | 1892.9808 | 1892.9672 | 7.19 | 0 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
WIFESVWAGLTELTNK | |
| 644.6515 | 1930.9327 | 1930.9272 | 2.83 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
DLDGGSKVISQWAPENSK + Deamidated (NQ) | |
| 756.8788 | 1511.7430 | 1511.7290 | 9.32 | 1 | 1 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
AMEELNYTKVNGK + Oxidation (M) | |
| 530.6088 | 1588.8046 | 1588.8130 | -5.33 | 1 | 1 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
LGAVTGLDLAQNCKK + 2 Deamidated (NQ) | |
| 768.3836 | 2302.1290 | 2302.1297 | -0.32 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
LIHMERDLSSEVVGQMDAIK + 2 Oxidation (M) | |
| 933.4354 | 1864.8562 | 1864.8890 | -17.5 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
WVRNLGNMNNVNLYR + 3 Deamidated (NQ) | |
| 743.4061 | 742.3988 | 742.3974 | 1.98 | 0 | 1 | 2.8 | 1Score > 18 indicates identity |
QGGSAPVK | |
| 380.2318 | 758.4490 | 758.4398 | 12.1 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
AIRNSAK | |
| 465.7309 | 929.4472 | 929.4566 | -10.1 | 0 | 1 | 3.7 | 1Score > 19 indicates identity |
IDGNEINR | |
| 1114.5276 | 1113.5203 | 1113.5376 | -15.5 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
LTAYNGMTVK + Deamidated (NQ); Oxidation (M) | |
| 1085.5422 | 2169.0698 | 2169.0841 | -6.56 | 1 | 1 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
SAEYSLKEIQIEELQDFK | |
| 529.7508 | 1057.4870 | 1057.5040 | -16.0 | 0 | 1 | 3.5 | 1Score > 18 indicates identity |
INDEAVSGPR + Deamidated (NQ) | |
| 521.6007 | 1561.7803 | 1561.7997 | -12.4 | 0 | 1 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
VLQTMSFLMNHLI + Oxidation (M) | |
| 792.4221 | 3165.6593 | 3165.6907 | -9.93 | 0 | 1 | 3.6 | 1Score > 19 indicates identity |
VLLIQNLPSGTTEQLLSQILGNEALVEIR + 5 Deamidated (NQ) | |
| 795.4116 | 794.4043 | 794.4188 | -18.2 | 0 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
GLFHGHK | |
| 794.0735 | 2379.1987 | 2379.1602 | 16.2 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
AMTIYWDDHTKKPLSMALNK + Deamidated (NQ); Oxidation (M) | |
| 392.2043 | 782.3940 | 782.3963 | -2.86 | 0 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
NGFFGLK + Deamidated (NQ) | |
| 899.4950 | 898.4877 | 898.4984 | -11.9 | 1 | 1 | 3.1 | 1Score > 18 indicates identity |
ERLQPTR | |
| 1081.5197 | 1080.5124 | 1080.5339 | -19.9 | 0 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
TLSLFEQNK + 2 Deamidated (NQ) | |
| 742.8682 | 1483.7218 | 1483.7453 | -15.8 | 0 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
GIANTHSSLICSKP | |
| 952.8108 | 2855.4106 | 2855.4328 | -7.77 | 0 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
EYVSLVGQVLLDGSSLSNEEILQFSK + 2 Deamidated (NQ) | |
| 1022.4603 | 2042.9060 | 2042.9353 | -14.3 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
AKLQVEQEQSLQNYMSK + 4 Deamidated (NQ); Oxidation (M) | |
| 1002.9807 | 2003.9468 | 2003.9225 | 12.2 | 1 | 1 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
EFYSSNKFVAGEAVADNR + Deamidated (NQ) | |
| 393.1978 | 784.3810 | 784.3967 | -19.9 | 0 | 1 | 4 | 1Score > 19 indicates identity |
QAPGLELG + Deamidated (NQ) | |
| 1064.5161 | 1063.5088 | 1063.5121 | -3.04 | 0 | 1 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
MGPSYVSAPR | |
| 657.8066 | 1313.5986 | 1313.6186 | -15.2 | 1 | 1 | 0.94 | 1Score > 20 indicates identityScore > 13 indicates homology |
FRMNFEQGLR + Deamidated (NQ); Oxidation (M) | |
| 929.4566 | 1856.8986 | 1856.9124 | -7.41 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
NVCLKNSEIIQICHK + 2 Deamidated (NQ) | |
| 911.9836 | 3643.9053 | 3643.9411 | -9.83 | 1 | 1 | 1.8 | 1Score > 16 indicates identity |
WPWRSTQWFMVILSGCNVILLTVLLPETLR + Oxidation (M) | |
| 382.6870 | 763.3594 | 763.3534 | 7.89 | 0 | 0 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
MATPSNK + Oxidation (M) | |
| 532.2686 | 1062.5226 | 1062.5346 | -11.2 | 0 | 0 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
LGGYPDVSAGK | |
| 717.9955 | 2150.9647 | 2150.9603 | 2.02 | 0 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
NLVLTQSNFNGGINNDNNAK + 5 Deamidated (NQ) | |
| 966.4651 | 965.4578 | 965.4641 | -6.45 | 0 | 0 | 1 | 1Score > 22 indicates identityScore > 13 indicates homology |
NVLDFCAK | |
| 878.4495 | 877.4422 | 877.4294 | 14.7 | 0 | 0 | 1 | 1Score > 24 indicates identityScore > 13 indicates homology |
RPYDAEK | |
| 487.5854 | 1459.7344 | 1459.7154 | 13.0 | 0 | 0 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
INGLNSNNSIIGNK + 3 Deamidated (NQ) | |
| 1112.4905 | 2222.9664 | 2222.9936 | -12.2 | 1 | 0 | 3.5 | 1Score > 18 indicates identity |
DQGMNRIGNLLVPNDNYCK + 3 Deamidated (NQ) | |
| 1090.8507 | 3269.5303 | 3269.5186 | 3.56 | 1 | 0 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
NLINIDKVPGWLAGENGSFSGTIMNGDFQR + 4 Deamidated (NQ); Oxidation (M) | |
| 943.4778 | 5654.8231 | 5654.7796 | 7.70 | 0 | 0 | 1.7 | 1Score > 15 indicates identity |
GWAPQVLVLDHPSLGCFLTHCGWNSTIEGITAGVPLITWPVFAEQFYNEK | |
| 696.3322 | 695.3249 | 695.3126 | 17.7 | 0 | 0 | 4.2 | 1Score > 19 indicates identity |
AADYQK + Deamidated (NQ) | |
| 545.2941 | 1088.5736 | 1088.5760 | -2.19 | 1 | 0 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
RVAAMSVAQR + Deamidated (NQ) | |
| 702.9949 | 2105.9629 | 2105.9914 | -13.6 | 1 | 0 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
FPQYKLTTFANQVMMDR + Deamidated (NQ); Oxidation (M) |
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