MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4601–4700 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4656 1141.8824 3422.6254
4657 1142.9113 3425.7121
4658 857.6805 3426.6929
4659 858.1825 3428.7009
4660 858.4344 3429.7085
4661 1144.2633 3429.7681
4662 1144.5930 3430.7572
4663 861.4434 3441.7445
4664 861.4472 3441.7597
4665 865.1866 3456.7173
4666 865.6826 3458.7013
4667 1155.2174 3462.6304
4668 1158.7897 3473.3473
4669 870.4360 3477.7149
4670 1160.5742 3478.7008
4671 1161.5800 3481.7182
4672 1161.5936 3481.7590
4673 872.4398 3485.7301
4674 1163.5564 3487.6474
4675 1165.5500 3493.6282
4676 1165.5665 3493.6777
4677 1165.5796 3493.7170
4678 1171.5276 3511.5610
4679 1176.2268 3525.6586
4680 1178.9020 3533.6842
4681 888.4321 3549.6993
4682 888.4351 3549.7113
4683 1184.5696 3550.6870
4684 1184.5803 3550.7191
4686 895.4395 3577.7289
4687 1193.5864 3577.7374
4688 906.4920 3621.9389
4689 910.4433 3637.7441
4691 913.4403 3649.7321
4692 913.9520 3651.7789
4693 914.1976 3652.7613
4694 916.9570 3663.7989
4695 919.4557 3673.7937
4696 920.4542 3677.7877
4697 920.7060 3678.7949
4698 924.2106 3692.8133
4699 926.4442 3701.7477
4700 926.9473 3703.7601
4701 927.4485 3705.7649
4702 930.1891 3716.7273
4704 938.7639 3751.0265
4705 986.7158 3942.8341
4706 990.4628 3957.8221
4707 1010.4946 4037.9493
4708 1016.5244 4062.0685
4709 1016.7730 4063.0629
4710 1017.0237 4064.0657
4711 1044.0552 4172.1917
4712 1045.8136 4179.2253
4714 1053.5260 4210.0749
4715 1057.5068 4225.9981
4716 1072.5229 4286.0625
4717 1086.2535 4340.9849
4718 1095.1036 4376.3853
4719 1104.0541 4412.1873
4720 1122.0552 4484.1917
4721 901.8760 4504.3436
4722 902.0709 4505.3181
4723 1127.3374 4505.3205
4724 1138.3511 4549.3753
4725 913.4816 4562.3716
4726 916.2742 4576.3346
4728 929.4678 4642.3026
4729 940.6677 4698.3021
4730 1100.3427 5496.6771
4731 922.9901 5531.8969
4732 1107.3877 5531.9021
4733 923.1547 5532.8845
4734 924.9911 5543.9029
4735 1110.1855 5545.8911
4736 925.3251 5545.9069
4737 1112.9908 5559.9176
4738 927.6606 5559.9199
4739 1113.1915 5560.9211
4740 927.8276 5560.9219
4742 798.5599 5582.8684
4743 1118.5380 5587.6536
4744 1118.7424 5588.6756
4746 800.7020 5597.8631
4747 934.8259 5602.9117
4748 934.9953 5603.9281
4749 936.4747 5612.8045
4750 937.1663 5616.9541
4751 937.3277 5617.9225
4752 1124.7902 5618.9146
4754 1005.7088 6028.2091
4755 863.3312 6036.2675
4756 1007.8762 6041.2135
4757 864.1814 6042.2189
4758 864.1815 6042.2196
4759 866.1842 6056.2385
4760 1010.5472 6057.2395
4761 866.3279 6057.2444
4762 1010.5516 6057.2659
4763 866.4738 6058.2657
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