MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4501–4600 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4548 1017.5416 3049.6030
4549 1017.8799 3050.6179
4550 1018.8448 3053.5126
4551 764.3872 3053.5197
4552 1019.4813 3055.4221
4553 1019.5143 3055.5211
4554 1020.8226 3059.4460
4555 1024.4584 3070.3534
4556 1024.5011 3070.4815
4557 1025.1423 3072.4051
4558 1025.8207 3074.4403
4559 1028.1741 3081.5005
4560 1028.5145 3082.5217
4561 1029.4897 3085.4473
4562 772.3838 3085.5061
4563 1029.5149 3085.5229
4564 776.1320 3100.4989
4565 1034.5099 3100.5079
4566 1034.5168 3100.5286
4568 776.3800 3101.4909
4569 1035.1730 3102.4972
4570 1035.8191 3104.4355
4572 1039.5106 3115.5100
4573 780.3830 3117.5029
4574 1040.1771 3117.5095
4575 1040.5094 3118.5064
4576 782.4099 3125.6105
4577 1046.1748 3135.5026
4578 1046.5087 3136.5043
4580 1049.5100 3145.5082
4581 1053.5179 3157.5319
4583 1053.8506 3158.5300
4584 1054.5128 3160.5166
4585 1055.5056 3163.4950
4586 792.4215 3165.6569
4588 792.4258 3165.6741
4589 792.6707 3166.6537
4590 1058.8488 3173.5246
4591 1059.5127 3175.5163
4592 1059.8527 3176.5363
4593 1062.4780 3184.4122
4594 1062.7947 3185.3623
4595 1065.5071 3193.4995
4596 1065.5480 3193.6222
4597 1066.5171 3196.5295
4598 1067.7920 3200.3542
4599 1067.7988 3200.3746
4601 1071.8455 3212.5147
4602 804.1369 3212.5185
4603 1071.8468 3212.5186
4604 804.3882 3213.5237
4605 1077.1776 3228.5110
4606 808.3756 3229.4733
4607 808.3852 3229.5117
4608 1079.8917 3236.6533
4610 1087.1943 3258.5611
4611 1088.5223 3262.5451
4612 1090.5820 3268.7242
4614 1091.8640 3272.5702
4615 1094.5392 3280.5958
4616 1095.5536 3283.6390
4617 1096.1857 3285.5353
4618 822.3925 3285.5409
4619 1096.2180 3285.6322
4620 1097.4767 3289.4083
4621 1097.4972 3289.4698
4622 1097.8186 3290.4340
4623 1098.8707 3293.5903
4624 1100.5610 3298.6612
4625 1102.2220 3303.6442
4626 829.8859 3315.5145
4627 1106.5652 3316.6738
4628 1107.5569 3319.6489
4629 1108.8721 3323.5945
4630 1111.4879 3331.4419
4631 833.8767 3331.4777
4632 1111.5037 3331.4893
4633 1111.8615 3332.5627
4634 1116.4923 3346.4551
4635 1116.8253 3347.4541
4636 1117.1494 3348.4264
4637 1117.1902 3348.5488
4638 1117.5216 3349.5430
4639 840.4171 3357.6393
4640 1123.2087 3366.6043
4641 1123.5693 3367.6861
4642 843.1767 3368.6777
4643 1124.1495 3369.4267
4644 1124.1527 3369.4363
4645 846.9229 3383.6625
4646 1128.8975 3383.6707
4647 1129.1340 3384.3802
4648 1129.4674 3385.3804
4649 1130.5375 3388.5907
4650 1132.8798 3395.6176
4651 1135.4896 3403.4470
4652 1136.1617 3405.4633
4653 1140.5522 3418.6348
4654 856.4128 3421.6221
4655 856.4238 3421.6661
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