MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4401–4500 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4444 962.4633 2884.3681
4445 962.7938 2885.3596
4446 962.8115 2885.4127
4447 965.1820 2892.5242
4448 967.4794 2899.4164
4449 580.8912 2899.4196
4450 968.1372 2901.3898
4451 971.1541 2910.4405
4452 729.6434 2914.5445
4453 973.7874 2918.3404
4454 973.8590 2918.5552
4455 975.4645 2923.3717
4456 975.4680 2923.3822
4457 975.5001 2923.4785
4458 980.4628 2938.3666
4459 980.7960 2939.3662
4460 981.1370 2940.3892
4461 981.1546 2940.4420
4462 736.1219 2940.4585
4463 981.1651 2940.4735
4464 981.4894 2941.4464
4465 982.4794 2944.4164
4466 982.4796 2944.4170
4467 737.1395 2944.5289
4468 982.5275 2944.5607
4469 982.8102 2945.4088
4470 983.4616 2947.3630
4471 983.4793 2947.4161
4472 738.8723 2951.4601
4473 984.8307 2951.4703
4474 739.8698 2955.4501
4475 739.9023 2955.5801
4476 986.4611 2956.3615
4477 986.4682 2956.3828
4478 986.4919 2956.4539
4479 986.7999 2957.3779
4480 986.8228 2957.4466
4481 740.3704 2957.4525
4482 988.4487 2962.3243
4483 988.4512 2962.3318
4484 988.4614 2962.3624
4485 989.8264 2966.4574
4486 989.8348 2966.4826
4487 990.1591 2967.4555
4488 742.8721 2967.4593
4489 990.1630 2967.4672
4490 742.8749 2967.4705
4492 990.4763 2968.4071
4493 990.5247 2968.5523
4494 743.9016 2971.5773
4495 744.3793 2973.4881
4496 992.8314 2975.4724
4499 993.1674 2976.4804
4500 993.4425 2977.3057
4501 993.7756 2978.3050
4502 994.4910 2980.4512
4503 994.8221 2981.4445
4504 746.3862 2981.5157
4505 995.1575 2982.4507
4506 746.6243 2982.4681
4507 995.4562 2983.3468
4508 995.4754 2983.4044
4509 995.4901 2983.4485
4511 995.4980 2983.4722
4512 748.3687 2989.4457
4513 998.4290 2992.2652
4514 998.4423 2992.3051
4515 998.7759 2993.3059
4516 999.4789 2995.4149
4517 999.8353 2996.4841
4518 750.1288 2996.4861
4519 1000.1442 2997.4108
4520 750.3681 2997.4433
4521 750.3804 2997.4925
4522 1000.4880 2998.4422
4523 1000.5014 2998.4824
4524 750.8726 2999.4613
4525 1002.4846 3004.4320
4526 752.3555 3005.3929
4527 1003.8358 3008.4856
4528 753.9083 3011.6041
4529 1005.4989 3013.4749
4530 754.3776 3013.4813
4531 1005.8281 3014.4625
4532 754.8747 3015.4697
4533 1006.2291 3015.6655
4534 755.3784 3017.4845
4535 756.3657 3021.4337
4536 1009.1670 3024.4792
4537 757.1279 3024.4825
4538 1009.1691 3024.4855
4539 1010.4435 3028.3087
4540 1010.5522 3028.6348
4541 1010.7800 3029.3182
4542 758.3395 3029.3289
4543 1011.1656 3030.4750
4544 1011.4952 3031.4638
4545 1014.4983 3040.4731
4546 1014.5064 3040.4974
4547 1015.5010 3043.4812
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