MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4301–4400 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4334 872.0652 2613.1738
4335 872.0671 2613.1795
4336 872.4219 2614.2439
4337 872.4224 2614.2454
4338 876.4152 2626.2238
4339 876.4326 2626.2760
4340 876.4424 2626.3054
4341 876.7320 2627.1742
4342 880.4662 2638.3768
4343 661.1201 2640.4513
4344 881.7271 2642.1595
4345 881.7347 2642.1823
4346 882.0598 2643.1576
4347 883.1040 2646.2902
4348 883.4681 2647.3825
4349 883.4725 2647.3957
4350 883.4780 2647.4122
4351 886.0950 2655.2632
4352 886.7199 2657.1379
4353 534.4866 2667.3966
4354 891.4327 2671.2763
4355 891.4414 2671.3024
4356 895.7690 2684.2852
4357 672.3437 2685.3457
4358 896.1318 2685.3736
4359 896.4492 2686.3258
4360 896.8386 2687.4940
4363 898.4478 2692.3216
4364 898.4542 2692.3408
4365 898.8415 2693.5027
4366 899.4218 2695.2436
4367 901.0739 2700.1999
4368 901.1003 2700.2791
4370 904.7524 2711.2354
4371 907.4247 2719.2523
4372 909.1777 2724.5113
4373 912.0894 2733.2464
4374 912.4542 2734.3408
4375 912.4649 2734.3729
4376 913.4568 2737.3486
4377 913.4665 2737.3777
4378 915.4415 2743.3027
4379 916.1740 2745.5002
4380 688.3871 2749.5193
4381 917.8426 2750.5060
4382 917.8429 2750.5069
4383 918.1733 2751.4981
4384 688.8855 2751.5129
4385 918.4286 2752.2640
4386 689.8436 2755.3453
4387 690.3391 2757.3273
4389 921.1024 2760.2854
4390 922.1373 2763.3901
4391 922.4698 2764.3876
4392 922.4724 2764.3954
4394 922.8319 2765.4739
4395 923.0765 2766.2077
4396 923.7789 2768.3149
4397 925.1671 2772.4795
4398 925.5139 2773.5199
4399 926.4421 2776.3045
4400 926.4446 2776.3120
4401 927.6617 2779.9633
4402 927.6661 2779.9765
4403 928.1171 2781.3295
4404 929.4645 2785.3717
4405 930.3244 2787.9514
4406 698.1209 2788.4545
4407 558.6987 2788.4571
4409 932.8011 2795.3815
4411 933.8042 2798.3908
4412 701.3378 2801.3221
4413 702.8910 2807.5349
4414 937.7522 2810.2348
4415 938.4464 2812.3174
4416 938.4569 2812.3489
4417 938.4657 2812.3753
4419 942.1309 2823.3709
4420 942.1320 2823.3742
4421 942.4712 2824.3918
4422 943.7790 2828.3152
4423 943.7838 2828.3296
4424 944.1227 2829.3463
4425 945.4388 2833.2946
4426 945.4724 2833.3954
4427 945.8572 2834.5498
4428 710.8572 2839.3997
4429 947.4790 2839.4152
4430 949.1185 2844.3337
4431 950.7611 2849.2615
4432 951.4448 2851.3126
4433 951.7875 2852.3407
4434 714.3625 2853.4209
4436 953.1116 2856.3130
4437 953.7991 2858.3755
4439 957.4576 2869.3510
4440 957.4626 2869.3660
4441 957.4691 2869.3855
4442 718.3818 2869.4981
4443 961.8168 2882.4286
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