MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4201–4300 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4224 806.0571 2415.1495
4225 604.8090 2415.2069
4226 604.8115 2415.2169
4228 807.3781 2419.1125
4230 808.7581 2423.2525
4232 809.3697 2425.0873
4233 809.3961 2425.1665
4234 809.3970 2425.1692
4235 810.1059 2427.2959
4236 810.3997 2428.1773
4237 810.7051 2429.0935
4238 608.3136 2429.2253
4239 811.3627 2431.0663
4240 608.8112 2431.2157
4241 811.4249 2431.2529
4242 813.7075 2438.1007
4243 813.7834 2438.3284
4244 814.0875 2439.2407
4245 814.3704 2440.0894
4246 814.3950 2440.1632
4247 814.3964 2440.1674
4248 814.4050 2440.1932
4249 612.8100 2447.2109
4250 816.7532 2447.2378
4251 817.1395 2448.3967
4252 817.4161 2449.2265
4253 819.0394 2454.0964
4254 819.4030 2455.1872
4255 819.4171 2455.2295
4257 819.4184 2455.2334
4258 820.0607 2457.1603
4260 823.1240 2466.3502
4261 824.3635 2470.0687
4262 618.8132 2471.2237
4263 824.7487 2471.2243
4264 619.0670 2472.2389
4265 825.3932 2473.1578
4266 826.3831 2476.1275
4267 826.7178 2477.1316
4268 827.1337 2478.3793
4269 828.4048 2482.1926
4270 829.0549 2484.1429
4271 829.7512 2486.2318
4273 623.0656 2488.2333
4274 830.4212 2488.2418
4275 830.4517 2488.3333
4276 832.3943 2494.1611
4278 832.4241 2494.2505
4279 832.7390 2495.1952
4280 834.3754 2500.1044
4281 834.3827 2500.1263
4282 834.3911 2500.1515
4284 834.7505 2501.2297
4285 835.0689 2502.1849
4286 836.0916 2505.2530
4287 836.4171 2506.2295
4288 837.3932 2509.1578
4289 837.4427 2509.3063
4290 838.0609 2511.1609
4291 838.4252 2512.2538
4292 838.7571 2513.2495
4293 630.0876 2516.3213
4294 839.7812 2516.3218
4295 841.4059 2521.1959
4296 842.4011 2524.1815
4297 842.4066 2524.1980
4298 843.0330 2526.0772
4299 843.0541 2526.1405
4301 843.7554 2528.2444
4302 843.7560 2528.2462
4303 846.4218 2536.2436
4304 848.0634 2541.1684
4305 848.4260 2542.2562
4306 849.0854 2544.2344
4307 849.0874 2544.2404
4308 849.3805 2545.1197
4310 851.3870 2551.1392
4311 853.4186 2557.2340
4312 854.7120 2561.1142
4313 857.4268 2569.2586
4314 857.4309 2569.2709
4315 858.3923 2572.1551
4316 860.4094 2578.2064
4317 861.4067 2581.1983
4318 646.8639 2583.4265
4319 862.3867 2584.1383
4320 862.4244 2584.2514
4321 862.4336 2584.2790
4322 863.3883 2587.1431
4323 863.3996 2587.1770
4324 866.0800 2595.2182
4325 866.4009 2596.1809
4326 866.4180 2596.2322
4327 866.7361 2597.1865
4328 867.0927 2598.2563
4329 867.0952 2598.2638
4330 868.0527 2601.1363
4331 869.0870 2604.2392
4332 869.4153 2605.2241
4333 871.7403 2612.1991
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