MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4108 1152.5789 2303.1432
4109 768.7230 2303.1472
4110 1153.5828 2305.1510
4111 769.3933 2305.1581
4112 1154.0643 2306.1140
4113 769.9908 2306.9506
4115 1155.0699 2308.1252
4117 770.3831 2308.1275
4118 770.3835 2308.1287
4119 770.3846 2308.1320
4120 770.3851 2308.1335
4121 770.4072 2308.1998
4122 1157.5620 2313.1094
4123 1157.5626 2313.1106
4124 772.0453 2313.1141
4125 772.0463 2313.1171
4126 772.3605 2314.0597
4127 773.3917 2317.1533
4128 774.0617 2319.1633
4129 775.3819 2323.1239
4130 775.3881 2323.1425
4131 775.4011 2323.1815 2323.1478 14.5 0     +0Score > 21 indicates identity
Score > 13 indicates homology		 TIMNIDATTEGENLHVLSPPR + Oxidation (M)
4132 1162.5994 2323.1842
4133 1163.0658 2324.1170
4134 1163.0681 2324.1216
4136 776.6820 2327.0242
4137 1164.5581 2327.1016
4140 1165.5555 2329.0964
4142 777.3784 2329.1134
4143 1168.5543 2335.0940
4144 779.6995 2336.0767
4145 780.1171 2337.3295
4147 780.3992 2338.1758
4148 780.7325 2339.1757
4149 1170.5969 2339.1792
4150 781.3669 2341.0789
4151 782.4059 2344.1959
4152 782.4064 2344.1974
4153 782.7009 2345.0809
4154 587.3055 2345.1929
4155 587.3055 2345.1929
4156 587.3060 2345.1949
4157 587.5266 2346.0773
4158 783.0403 2346.0991
4159 784.3589 2350.0549
4160 784.3978 2350.1716
4161 1176.5474 2351.0802
4162 784.7017 2351.0833
4163 785.0716 2352.1930
4164 785.3699 2353.0879
4165 785.3923 2353.1551
4166 1178.5964 2355.1782
4168 786.3853 2356.1341
4169 591.2869 2361.1185
4170 591.5247 2362.0697
4171 788.3668 2362.0786
4172 788.3824 2362.1254
4173 789.0609 2364.1609
4174 1183.5741 2365.1336
4175 789.3901 2365.1485
4176 789.4017 2365.1833
4177 1184.5452 2367.0758
4178 790.0327 2367.0763
4179 791.0597 2370.1573
4180 791.3892 2371.1458
4181 791.3977 2371.1713
4182 594.0546 2372.1893
4183 594.0567 2372.1977
4185 793.0708 2376.1906
4186 793.3804 2377.1194
4187 793.3965 2377.1677
4188 793.3985 2377.1737
4189 793.4045 2377.1917
4191 1191.0807 2380.1468
4192 794.3928 2380.1566
4193 1191.1064 2380.1982
4194 794.4123 2380.2151
4195 794.7204 2381.1394
4196 795.0318 2382.0736
4197 1192.0714 2382.1282
4198 795.6993 2384.0761
4199 796.3660 2386.0762
4200 796.6887 2387.0443
4202 797.3966 2389.1680
4207 599.3045 2393.1889
4208 799.4002 2395.1788
4209 799.4084 2395.2034
4210 800.0286 2397.0640
4211 800.3904 2398.1494
4212 800.3921 2398.1545
4213 800.4326 2398.2760
4214 600.8097 2399.2097
4215 600.8110 2399.2149
4216 801.4074 2401.2004
4217 601.3115 2401.2169
4218 801.4158 2401.2256
4219 801.7440 2402.2102
4220 601.5617 2402.2177
4221 803.7085 2408.1037
4223 805.3602 2413.0588
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