MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3998 1105.9958 2209.9770
3999 1106.5312 2211.0478
4000 738.3330 2211.9772
4001 1107.0016 2211.9886
4002 1107.4874 2212.9602
4003 738.6618 2212.9636
4005 1108.4912 2214.9678
4006 739.6546 2215.9420
4007 1108.9791 2215.9436
4008 1109.0149 2216.0152
4010 1109.5400 2217.0654
4011 1110.0323 2218.0500
4012 740.3582 2218.0528
4013 1110.0344 2218.0542
4014 741.3444 2221.0114
4015 1111.5132 2221.0118
4016 741.6721 2221.9945
4017 1112.0237 2222.0328
4018 741.6852 2222.0338
4020 1112.5020 2222.9894
4021 742.0041 2222.9905
4022 1114.5345 2227.0544
4023 743.3595 2227.0567
4024 743.6625 2227.9657
4025 1115.5238 2229.0330
4026 744.3636 2230.0690
4027 1116.4838 2230.9530
4028 744.6592 2230.9558
4029 1117.4838 2232.9530
4030 1117.5255 2233.0364
4031 1118.0176 2234.0206
4032 745.6818 2234.0236
4033 1120.5016 2238.9886
4034 1120.5259 2239.0372
4035 747.4001 2239.1785
4036 1120.9894 2239.9642
4037 747.6639 2239.9699
4038 747.6700 2239.9882
4039 748.9944 2243.9614
4040 1123.5544 2245.0942
4041 749.7543 2246.2411
4042 750.3756 2248.1050
4043 1125.5112 2249.0078
4044 750.7051 2249.0935
4045 1126.0601 2250.1056
4046 1126.0631 2250.1116
4047 751.0457 2250.1153
4048 751.3770 2251.1092
4049 751.3776 2251.1110
4050 751.3848 2251.1326
4051 751.7754 2252.3044
4052 751.7785 2252.3137
4053 752.0122 2253.0148
4054 1127.5162 2253.0178
4055 1127.5176 2253.0206
4056 752.3736 2254.0990
4057 755.0502 2262.1288
4058 1133.5468 2265.0790
4059 756.3808 2266.1206
4060 1134.5566 2267.0986
4061 1134.5591 2267.1036
4062 1135.5054 2268.9962
4063 757.3402 2268.9988
4064 1135.5120 2269.0094
4065 1136.5134 2271.0122
4066 1136.5457 2271.0768
4067 1136.5509 2271.0872
4068 1136.9988 2271.9830
4069 758.3434 2272.0084
4070 758.3660 2272.0762
4071 1138.5469 2275.0792
4072 759.3685 2275.0837
4073 759.3868 2275.1386
4075 1141.5895 2281.1644
4076 1141.6056 2281.1966
4078 1145.5627 2289.1108
4080 764.7213 2291.1421
4081 1146.5791 2291.1436
4082 1147.0730 2292.1314
4083 765.0513 2292.1321
4084 765.0880 2292.2422
4085 1147.5284 2293.0422
4086 765.4169 2293.2289
4087 765.4175 2293.2307
4088 765.4187 2293.2343
4089 765.4193 2293.2361
4090 765.4200 2293.2382
4091 765.4211 2293.2415
4092 765.4233 2293.2481
4093 765.4246 2293.2520
4094 765.4278 2293.2616
4095 766.3377 2295.9913
4096 766.3751 2296.1035
4097 1149.5016 2296.9886
4098 1149.5211 2297.0276
4099 767.3527 2299.0363
4102 768.3833 2302.1281
4104 768.3914 2302.1524
4105 768.3967 2302.1683
4106 1152.5538 2303.0930
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