MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3880 1067.5226 2133.0306
3882 1068.4915 2134.9684
3883 712.6635 2134.9687
3884 1068.4938 2134.9730
3885 712.6650 2134.9732
3886 1068.5024 2134.9902
3889 712.6919 2135.0539
3890 712.6991 2135.0755
3891 713.0099 2136.0079
3892 1069.0464 2136.0782
3893 713.3255 2136.9547
3894 1069.4863 2136.9580
3895 1069.4866 2136.9586
3896 1069.5171 2137.0196
3897 713.3682 2137.0828
3898 1069.5491 2137.0836
3899 1071.4907 2140.9668
3900 1072.5259 2143.0372
3902 536.9880 2143.9229
3903 1073.4985 2144.9824
3904 1073.5212 2145.0278
3905 716.6400 2146.8982
3906 1075.4993 2148.9840
3907 717.3355 2148.9847
3908 1075.5105 2149.0064
3909 1075.9857 2149.9568
3910 1076.0142 2150.0138
3911 717.6852 2150.0338
3912 1076.0369 2150.0592
3914 1076.5137 2151.0128
3915 1076.5256 2151.0366
3916 718.0212 2151.0418
3917 1076.5452 2151.0758
3918 718.0331 2151.0775
3919 718.3602 2152.0588
3920 1077.4834 2152.9522
3921 1077.4836 2152.9526
3922 718.9948 2153.9626
3923 719.6620 2155.9642
3924 719.9810 2156.9212
3925 1079.4799 2156.9452
3926 719.9896 2156.9470
3927 1079.5355 2157.0564
3928 720.3696 2158.0870
3929 720.3774 2158.1104
3930 1081.4961 2160.9776
3932 1082.0614 2162.1082
3934 1082.4988 2162.9830
3935 1082.5166 2163.0186
3936 1083.0002 2163.9858
3937 722.3395 2163.9967
3938 722.3407 2164.0003
3941 1083.4937 2164.9728
3942 722.6660 2164.9762
3943 1083.5115 2165.0084
3944 1083.9799 2165.9452
3945 1085.4807 2168.9468
3946 1085.5316 2169.0486
3949 1085.9884 2169.9622
3950 1086.5135 2171.0124
3951 1086.5264 2171.0382
3953 1086.5408 2171.0670
3954 724.9761 2171.9065
3955 1087.4565 2172.8984
3956 725.3483 2173.0231
3957 725.6854 2174.0344
3958 1088.5245 2175.0344
3959 726.3778 2176.1116
3960 1089.5591 2177.1036
3961 1090.5049 2178.9952
3962 1091.5477 2181.0808
3964 1092.0479 2182.0812
3965 1093.5211 2185.0276
3966 1093.9833 2185.9520
3967 729.6600 2185.9582
3968 1094.5001 2186.9856
3969 1094.5045 2186.9944
3970 730.3325 2187.9757
3971 548.2533 2188.9841
3972 1096.5626 2191.1106
3973 731.3796 2191.1170
3975 731.6948 2192.0626
3977 1098.4989 2194.9832
3978 1098.9940 2195.9734
3979 733.0024 2195.9854
3980 1099.0054 2195.9962
3981 733.3353 2196.9841
3982 733.6628 2197.9666
3983 1100.4911 2198.9676
3985 734.3622 2200.0648
3986 1101.5402 2201.0658
3987 734.7388 2201.1946
3988 735.0300 2202.0682
3989 735.0318 2202.0736
3990 1102.0444 2202.0742
3991 735.3238 2202.9496
3993 735.3320 2202.9742
3994 735.3542 2203.0408
3996 1105.0378 2208.0610
3997 737.0283 2208.0631
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