MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4709)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3332 912.4158 1822.8170 1822.8155 0.83 1 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
NNFKIIDSHSMNNSGK + 2 Deamidated (NQ); Oxidation (M)
2384 582.2949 1162.5752 1162.5982 -19.8 0 4 0.73 +1Score > 23 indicates identity
Score > 15 indicates homology
LQTNQIFNGK + Deamidated (NQ)
981 328.1926 654.3706 654.3701 0.90 0 4 0.58 +1Score > 14 indicates identity TAAPAPK
1516 938.4218 937.4145 937.4215 -7.45 0 4 2.1 +1Score > 20 indicates identity WAVNTLMS + Deamidated (NQ); Oxidation (M)
1729 999.4761 998.4688 998.4781 -9.32 0 4 2.1 +1Score > 20 indicates identity EGVGPNVGDR
2459 1180.5582 1179.5509 1179.5706 -16.7 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
ASYNPIMTQR
1128 762.3447 761.3374 761.3378 -0.45 0 4 1.8 +1Score > 19 indicates identity ENSGPMK
4222 804.7550 2411.2432 2411.2067 15.1 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
IEAALSDALAALQIEDPSADELR + Deamidated (NQ)
2063 544.2852 1086.5558 1086.5570 -1.09 1 4 0.88 +1Score > 22 indicates identity
Score > 16 indicates homology
DGLLGWDRR
2624 656.2980 1310.5814 1310.6037 -17.0 1 4 2 +1Score > 19 indicates identity DHHKMAISDGGK + Oxidation (M)
3350 460.2230 1836.8629 1836.8490 7.58 0 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
DVGQDGLQAAISYATGNR + 2 Deamidated (NQ)
3005 530.9439 1589.8099 1589.7970 8.08 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
EAQRIEGMLQLLTS + 2 Deamidated (NQ)
1318 430.2115 858.4084 858.4083 0.20 0 4 1.7 +1Score > 18 indicates identity DANGKPEK + Deamidated (NQ)
1312 858.4193 857.4120 857.4171 -5.88 0 4 1.6 +1Score > 18 indicates identity YLSNIFT + Deamidated (NQ)
1063 724.4109 723.4036 723.4140 -14.3 1 4 1.5 +1Score > 18 indicates identity GNKHLR
2135 1103.5082 1102.5009 1102.5142 -12.0 0 4 1.9 +1Score > 19 indicates identity EGGETQEKPK + Deamidated (NQ)
1307 428.7305 855.4464 855.4338 14.8 0 4 1.6 +1Score > 18 indicates identity TPETSPPK
3020 801.9153 1601.8160 1601.8382 -13.8 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
STVPIIIRCCIDR
2739 717.3445 1432.6744 1432.7020 -19.2 1 4 0.67 +1Score > 21 indicates identity
Score > 14 indicates homology
MSFNAFASSLSKK + Oxidation (M)
3683 685.0151 2052.0235 2052.0561 -15.9 1 3 0.67 +1Score > 21 indicates identity
Score > 14 indicates homology
VINKCTSDIQLNSGIVYK + Deamidated (NQ)
3780 1045.9911 2089.9676 2089.9803 -6.08 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
LNLNNKPSQQQFSDPETK + 3 Deamidated (NQ)
3974 731.6685 2191.9837 2191.9954 -5.35 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
NANLNNSRNANAPGEAGHQNK + 2 Deamidated (NQ)
1263 419.2062 836.3978 836.4140 -19.4 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
HLDAEPR
1618 966.4652 965.4579 965.4679 -10.3 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
LSHENNPR
2266 1137.5419 1136.5346 1136.5132 18.9 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
AAERDNMLTT + Oxidation (M)
1908 524.7842 1047.5538 1047.5634 -9.12 0 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
NIVNLMISK + Deamidated (NQ); Oxidation (M)
1140 385.1961 768.3776 768.3878 -13.2 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
GNKANHK + Deamidated (NQ)
2654 667.8423 1333.6700 1333.6547 11.5 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
MALGNEINITNK + Deamidated (NQ); Oxidation (M)
3042 810.4078 1618.8010 1618.7739 16.7 0 3 0.52 +1Score > 23 indicates identity
Score > 13 indicates homology
LITAYYNEHGPGQR + Deamidated (NQ)
963 321.1849 640.3552 640.3657 -16.3 0 3 0.95 +1Score > 16 indicates identity VGVNPR
1071 728.3975 727.3902 727.3977 -10.3 0 3 1.3 +1Score > 17 indicates identity AVPGATGR
3564 992.9846 1983.9546 1983.9497 2.48 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
TALQQQIHAAQQATNTTR + 4 Deamidated (NQ)
1467 460.7513 919.4880 919.4909 -3.12 1 3 0.88 +1Score > 21 indicates identity
Score > 15 indicates homology
MTQRQLK + Oxidation (M)
1430 907.4361 906.4288 906.4406 -13.0 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
NKDSASASK
2864 504.2526 1509.7360 1509.7212 9.78 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
GVERPSTPFSDYR
1223 406.2201 810.4256 810.4348 -11.3 0 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
VSPNAAPR
3616 1006.5054 2010.9962 2011.0149 -9.29 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
LYQEEDLKNFEIQTLK + Deamidated (NQ)
3399 621.6647 1861.9723 1861.9898 -9.40 0 3 0.77 +1Score > 21 indicates identity
Score > 15 indicates homology
VVSQSQIIFTTNIAAGGR + Deamidated (NQ)
2653 667.8421 1333.6696 1333.6547 11.2 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
VENGIMQSISEK
4685 892.4351 3565.7113 3565.7093 0.55 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
GVQSPPKPPPSNEISGTIENDVESIKQAADNMAK + 2 Deamidated (NQ); Oxidation (M)
1372 884.4353 883.4280 883.4148 15.0 0 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
TVQNHQR + 2 Deamidated (NQ)
2800 733.3707 1464.7268 1464.7361 -6.32 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
YALATGNWGEQKK
1471 921.4510 920.4437 920.4273 17.8 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
SMENVTPK + Oxidation (M)
3530 655.6334 1963.8784 1963.9044 -13.3 0 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
AANLGGVAVSGLEMAQNSQK + 4 Deamidated (NQ); Oxidation (M)
1276 842.4385 841.4312 841.4406 -11.1 1 3 1.6 +1Score > 18 indicates identity QNPAERK
1446 911.4600 910.4527 910.4508 2.09 1 3 2.4 +1Score > 20 indicates identity ETYNTKR
1201 400.7176 799.4206 799.4301 -11.8 0 3 1.9 +1Score > 18 indicates identity LLGGGGGGGR
937 312.1794 622.3442 622.3551 -17.4 0 3 0.9 +1Score > 22 indicates identity
Score > 15 indicates homology
HNKPK
2134 552.2557 1102.4968 1102.5142 -15.7 0 3 2.2 +1Score > 19 indicates identity EGGETQEKPK + Deamidated (NQ)
1267 420.7331 839.4516 839.4501 1.84 0 3 1.9 +1Score > 18 indicates identity NATPVNPK
1304 855.4029 854.3956 854.3882 8.66 0 3 1.7 +1Score > 18 indicates identity NSSYTQR
1803 511.2798 1020.5450 1020.5492 -4.03 0 3 0.81 +1Score > 22 indicates identity
Score > 15 indicates homology
VTLFNTTPK + Deamidated (NQ)
3130 842.9015 1683.7884 1683.7661 13.3 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
QIQEYESMNGNLIK + 2 Deamidated (NQ); Oxidation (M)
1718 498.7426 995.4706 995.4712 -0.58 0 3 0.82 +1Score > 21 indicates identity
Score > 15 indicates homology
LEFGNYPR + Deamidated (NQ)
2087 1092.5162 1091.5089 1091.5108 -1.72 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
QPPETNHNR
2888 764.3556 1526.6966 1526.7221 -16.7 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
GNSMLFTMNRDIK + Deamidated (NQ)
982 328.1929 654.3712 654.3701 1.80 0 3 0.71 +1Score > 14 indicates identity SVPAPGK
1530 940.4722 939.4649 939.4484 17.6 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
FNKMNLR + 2 Deamidated (NQ); Oxidation (M)
3696 515.2592 2057.0077 2057.0331 -12.3 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
EPGTVFHVQQLGFTLNDR
2967 524.2629 1569.7669 1569.7787 -7.54 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
HDPLNSNIFSEAVK
2226 1125.5323 1124.5250 1124.5396 -13.0 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
AEPRHMQQK + Deamidated (NQ)
2928 774.4020 1546.7894 1546.7892 0.15 1 3 0.79 +1Score > 22 indicates identity
Score > 14 indicates homology
GLVVSPYDYNHKR
1517 469.7158 937.4170 937.4327 -16.7 1 3 2.6 +1Score > 20 indicates identity GEMKSGWK + Oxidation (M)
4498 992.8351 2975.4835 2975.4288 18.4 0 3 0.77 +1Score > 21 indicates identity
Score > 14 indicates homology
TIHIIEVDESFDATLSDDVVDQWLSK + Deamidated (NQ)
1112 757.4155 756.4082 756.4018 8.54 0 3 2.5 +1Score > 19 indicates identity TSPLPDK
1717 996.4777 995.4704 995.4560 14.5 0 3 0.65 +1Score > 21 indicates identity
Score > 13 indicates homology
QGLEGNSYK + Deamidated (NQ)
2912 769.8645 1537.7144 1537.7233 -5.75 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
RPDNQNPEGENLR
2895 765.9095 1529.8044 1529.8300 -16.7 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
KISQLVQENVTLR + 3 Deamidated (NQ)
1555 948.4549 947.4476 947.4308 17.8 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
NNESNNKK + Deamidated (NQ)
4114 1154.5721 2307.1296 2307.0980 13.7 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
NKGLTYNDTMLYYLNLPQK + 3 Deamidated (NQ); Oxidation (M)
1181 794.3692 793.3619 793.3640 -2.61 0 3 0.72 +1Score > 21 indicates identity
Score > 14 indicates homology
QLGDSMK + Oxidation (M)
4231 809.3671 2425.0795 2425.1049 -10.5 1 3 2.4 +1Score > 19 indicates identity LMENHACNGLTSCKEQLHQR
3641 1013.4971 2024.9796 2024.9837 -1.98 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
NIGVADPQNSLNPSKPNMK + 2 Deamidated (NQ)
1158 391.2019 780.3892 780.3766 16.2 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
PAPQDPR + Deamidated (NQ)
3162 852.9709 1703.9272 1703.9260 0.75 0 3 2.4 +1Score > 19 indicates identity QHIHYLNLLIHFR + Deamidated (NQ)
1995 1068.5188 1067.5115 1067.5247 -12.4 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
HQPITSEQK + Deamidated (NQ)
3269 889.4378 1776.8610 1776.8936 -18.3 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
ILQMGAIMNLGMPSRK + 2 Deamidated (NQ); Oxidation (M)
4074 761.0724 2280.1954 2280.1824 5.70 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
GMILGNFSILVASYANEVIPR + Deamidated (NQ); Oxidation (M)
2714 703.3409 1404.6672 1404.6885 -15.1 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
NPGSINIYEIER + Deamidated (NQ)
2508 600.2719 1198.5292 1198.5466 -14.4 1 3 2.3 +1Score > 19 indicates identity EPKNENDEPK
4229 808.1061 2421.2965 2421.2863 4.19 1 3 1.5 +1Score > 17 indicates identity LLIGKSSNSHSTDSATLVNVHIK + Deamidated (NQ)
2959 782.3888 1562.7630 1562.7551 5.07 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
DFLSIRYSMPYR + Oxidation (M)
4256 819.4179 2455.2319 2455.2496 -7.20 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
GLPSEKIHDVLGYSVSEYVAHR
4101 1152.0642 2302.1138 2302.1515 -16.4 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
LSMESPHVNVFSILTDLDIR + Deamidated (NQ); Oxidation (M)
1048 701.3663 700.3590 700.3504 12.3 0 3 1.2 +1Score > 16 indicates identity ASNQPGK
2642 664.8313 1327.6480 1327.6408 5.46 0 3 0.61 +1Score > 22 indicates identity
Score > 13 indicates homology
NSATILNYFER + Deamidated (NQ)
953 634.3574 633.3501 633.3520 -2.92 0 3 0.81 +1Score > 22 indicates identity
Score > 14 indicates homology
VGSLMK
3435 473.9743 1891.8681 1891.8986 -16.1 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
DSCKNIEPIDTPISFR + Deamidated (NQ)
4600 1070.8766 3209.6080 3209.6642 -17.5 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
EASEILAGLQGILPMDISVHQVDGGLKVYR + 2 Deamidated (NQ)
931 618.2739 617.2666 617.2769 -16.6 0 2 0.65 +1Score > 13 indicates identity QQGER + Deamidated (NQ)
1187 398.2225 794.4304 794.4174 16.4 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
GVEYSLK
2818 740.3571 1478.6996 1478.7286 -19.6 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
TSMSIIDDENVKK
3094 828.9061 1655.7976 1655.7647 19.9 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
EGAQLYSCAKCQLK + Deamidated (NQ)
3541 656.9966 1967.9680 1967.9550 6.59 1 2 0.62 +1Score > 22 indicates identity
Score > 13 indicates homology
LTNEDPPGLMYLKAFDK + Deamidated (NQ); Oxidation (M)
4703 934.2245 3732.8689 3732.8808 -3.19 1 2 2.7 +1Score > 19 indicates identity IKQVSLTNLQDDWMGVILVNSTQSDPLINTPFK + 3 Deamidated (NQ); Oxidation (M)
3864 708.6938 2123.0596 2123.0351 11.5 1 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
LNRQGTMVATCSVQGTLIR + 3 Deamidated (NQ); Oxidation (M)
1313 858.4222 857.4149 857.4171 -2.50 0 2 2 +1Score > 18 indicates identity YLSNIFT + Deamidated (NQ)
2717 705.8546 1409.6946 1409.7006 -4.25 1 2 0.79 +1Score > 21 indicates identity
Score > 14 indicates homology
NTNISIMRLGMK + Deamidated (NQ); 2 Oxidation (M)
1431 907.4511 906.4438 906.4308 14.4 0 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
NSNVSHHL
3144 564.2997 1689.8773 1689.8542 13.7 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
KIICPSVESISNGMR
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