MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3773 696.0065 2084.9977
3774 1043.5078 2085.0010
3775 1044.0922 2086.1698
3776 1044.4327 2086.8508
3777 1044.4922 2086.9698
3778 697.6627 2089.9663
3779 1045.9905 2089.9664
3781 1046.0000 2089.9854
3782 698.3263 2091.9571
3783 698.6538 2092.9396
3784 1047.4886 2092.9626
3785 1047.4937 2092.9728
3786 524.4935 2093.9449
3787 699.3624 2095.0654
3788 525.0040 2095.9869
3789 699.6697 2095.9873
3790 1049.4760 2096.9374
3791 1049.5178 2097.0210
3792 700.0220 2097.0442
3793 1049.5315 2097.0484
3794 1049.5337 2097.0528
3795 700.3212 2097.9418
3796 1049.9999 2097.9852
3797 1050.5063 2098.9980
3798 1051.0148 2100.0150
3799 1051.5049 2100.9952
3800 1051.5051 2100.9956
3802 701.3474 2101.0204
3803 701.3667 2101.0783
3804 701.3676 2101.0810
3805 701.3702 2101.0888
3806 701.6660 2101.9762
3807 701.6823 2102.0251
3808 701.6977 2102.0713
3809 701.9961 2102.9665
3810 1052.4968 2102.9790
3811 702.3344 2103.9814
3812 702.6611 2104.9615
3813 1053.4956 2104.9766
3814 1053.4958 2104.9770
3815 1053.5100 2105.0054
3816 1053.9882 2105.9618
3817 1053.9882 2105.9618
3819 1053.9923 2105.9700
3820 1053.9939 2105.9732
3821 702.9985 2105.9737
3822 703.3315 2106.9727
3823 703.6663 2107.9771
3824 703.9899 2108.9479
3825 1055.4813 2108.9480
3826 1055.4828 2108.9510
3827 703.9922 2108.9548
3828 1055.5137 2109.0128
3830 1056.4510 2110.8874
3831 1056.5349 2111.0552
3832 1056.9702 2111.9258
3833 705.0257 2112.0553
3834 705.0278 2112.0616
3835 1057.4935 2112.9724
3837 1057.5120 2113.0094
3838 1057.5160 2113.0174
3839 1057.5175 2113.0204
3840 705.3493 2113.0261
3841 1058.0260 2114.0374
3842 705.6870 2114.0392
3844 706.0570 2115.1492
3845 1058.5844 2115.1542
3846 706.3384 2115.9934
3847 1059.5070 2116.9994
3848 707.3284 2118.9634
3849 707.3317 2118.9733
3850 1060.4945 2118.9744
3851 1060.4956 2118.9766
3852 707.3329 2118.9769
3853 1060.5031 2118.9916
3854 1060.9884 2119.9622
3855 1061.4868 2120.9590
3856 707.9947 2120.9623
3857 1061.4889 2120.9632
3858 708.3281 2121.9625
3859 708.3527 2122.0363
3860 1062.5233 2123.0320
3861 1062.5261 2123.0376
3862 708.6873 2123.0401
3863 1062.5310 2123.0474
3865 1062.5465 2123.0784
3866 708.9973 2123.9701
3867 709.0257 2124.0553
3868 1063.4814 2124.9482
3869 1064.0239 2126.0332
3870 1064.5253 2127.0360
3871 1064.9960 2127.9774
3872 1066.0157 2130.0168
3873 1066.4707 2130.9268
3874 1066.5104 2131.0062
3875 1066.5171 2131.0196
3876 1066.5187 2131.0228
3877 711.6671 2131.9795
3878 712.0048 2132.9926
3879 1067.5049 2132.9952
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