MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3663 1021.4916 2040.9686
3664 1021.4932 2040.9718
3666 681.3552 2041.0438
3667 1021.9315 2041.8484
3668 1021.9431 2041.8716
3669 1021.9505 2041.8864
3671 1022.4981 2042.9816
3672 682.3345 2043.9817
3673 1023.4940 2044.9734
3674 1023.5007 2044.9868
3675 682.9798 2045.9176
3676 1023.9805 2045.9464
3677 1024.4739 2046.9332
3678 683.6507 2047.9303
3679 1026.4725 2050.9304
3682 1026.9843 2051.9540
3684 1027.5259 2053.0372
3687 1028.5343 2055.0540
3688 1029.0076 2056.0006
3689 1029.0081 2056.0016
3690 686.3430 2056.0072
3691 686.3433 2056.0081
3692 686.3433 2056.0081
3693 1029.4869 2056.9592
3694 686.6605 2056.9597
3695 1029.4883 2056.9620
3697 1029.5200 2057.0254
3698 1030.0260 2058.0374
3699 1030.4360 2058.8574
3700 687.2958 2058.8656
3701 687.3544 2059.0414
3702 1030.5299 2059.0452
3703 687.3569 2059.0489
3704 1030.9292 2059.8438
3705 1031.0375 2060.0604
3706 688.3015 2061.8827
3707 1031.9590 2061.9034
3708 688.3118 2061.9136
3709 1031.9795 2061.9444
3710 688.6458 2062.9156
3711 1032.4663 2062.9180
3712 1032.4792 2062.9438
3713 688.9832 2063.9278
3714 1033.4694 2064.9242
3715 689.3207 2064.9403
3716 1033.5045 2064.9944
3717 689.3572 2065.0498
3718 689.6741 2066.0005
3719 1034.5164 2067.0182
3720 1034.9757 2067.9368
3721 1035.4890 2068.9634
3722 1035.5132 2069.0118
3723 1036.4940 2070.9734
3724 691.3338 2070.9796
3725 1036.5009 2070.9872
3726 1037.4935 2072.9724
3727 1037.5062 2072.9978
3728 1037.5498 2073.0850
3729 1037.5535 2073.0924
3730 1037.5547 2073.0948
3732 1038.0459 2074.0772
3733 692.3679 2074.0819
3734 1038.0515 2074.0884
3735 1038.0538 2074.0930
3736 1038.0538 2074.0930
3737 692.3722 2074.0948
3738 1038.0549 2074.0952
3739 1038.0560 2074.0974
3740 692.3778 2074.1116
3741 1038.4321 2074.8496
3742 692.7207 2075.1403
3743 692.7212 2075.1418
3744 692.9958 2075.9656
3745 1038.9907 2075.9668
3746 693.6315 2077.8727
3747 1039.9557 2077.8968
3748 1039.9775 2077.9404
3749 693.6558 2077.9456
3750 1040.4604 2078.9062
3751 693.9787 2078.9143
3752 1040.4716 2078.9286
3753 1040.4767 2078.9388
3754 1040.5002 2078.9858
3755 694.3198 2079.9376
3756 1040.9778 2079.9410
3757 694.6682 2080.9828
3758 1042.0037 2081.9928
3759 1042.4982 2082.9818
3760 1042.5100 2083.0054
3761 695.3430 2083.0072
3762 1042.5121 2083.0096
3764 1042.5166 2083.0186
3765 1042.5167 2083.0188
3766 695.3504 2083.0294
3767 1042.5312 2083.0478
3768 1043.4834 2084.9522
3769 695.9963 2084.9671
3770 1043.4941 2084.9736
3771 1043.4955 2084.9764
3772 1043.4993 2084.9840
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