MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3551 989.4982 1976.9818
3552 990.4700 1978.9254
3553 990.4818 1978.9490
3554 660.9830 1979.9272
3555 991.4448 1980.8750
3556 991.4775 1980.9404
3560 661.6446 1981.9120
3561 661.9880 1982.9422
3562 992.9781 1983.9416
3563 992.9794 1983.9442
3565 994.5145 1987.0144
3566 663.6781 1988.0125
3567 995.4502 1988.8858
3568 498.2405 1988.9329
3569 995.9516 1989.8886
3570 664.3076 1989.9010
3572 995.9703 1989.9260
3573 664.3196 1989.9370
3574 995.9866 1989.9586
3575 996.4634 1990.9122
3576 997.4984 1992.9822
3577 665.6697 1993.9873
3578 998.0054 1993.9962
3579 998.4628 1994.9110
3580 665.9842 1994.9308
3581 998.4733 1994.9320
3582 998.4757 1994.9368
3583 998.4791 1994.9436
3584 998.4996 1994.9846
3585 1000.4697 1998.9248
3586 1000.4739 1998.9332
3587 1000.4750 1998.9354
3588 1000.5003 1998.9860
3589 667.6541 1999.9405
3590 1002.4843 2002.9540
3591 1002.4858 2002.9570
3592 668.9689 2003.8849
3593 1002.9631 2003.9116
3596 1003.4505 2004.8864
3597 1003.4700 2004.9254
3598 669.3414 2005.0024
3599 1003.9526 2005.8906
3600 669.6395 2005.8967
3601 1004.4797 2006.9448
3602 1004.4839 2006.9532
3603 1004.4917 2006.9688
3604 1004.9590 2007.9034
3605 670.3271 2007.9595
3606 670.3324 2007.9754
3607 670.3347 2007.9823
3608 670.3414 2008.0024
3609 1005.0112 2008.0078
3610 1005.4946 2008.9746
3611 1005.4962 2008.9778
3612 670.6674 2008.9804
3613 1005.5016 2008.9886
3614 1005.5135 2009.0124
3615 1005.5166 2009.0186
3617 1008.4650 2014.9154
3618 1008.4743 2014.9340
3619 672.6674 2014.9804
3620 1008.5051 2014.9956
3621 1009.4651 2016.9156
3624 674.0002 2018.9788
3625 674.0279 2019.0619
3626 674.0280 2019.0622
3627 674.3284 2019.9634
3628 1011.4469 2020.8792
3629 674.6345 2020.8817
3630 1011.4596 2020.9046
3631 1011.4711 2020.9276
3632 674.6500 2020.9282
3633 1011.4752 2020.9358
3634 1011.4883 2020.9620
3636 674.9705 2021.8897
3637 1013.4770 2024.9394
3638 1013.4926 2024.9706
3639 675.9977 2024.9713
3640 675.9979 2024.9719
3642 1013.5046 2024.9946
3643 1013.5076 2025.0006
3644 1014.5029 2026.9912
3645 1014.5067 2026.9988
3646 1015.4766 2028.9386
3647 677.3541 2029.0405
3648 677.6851 2030.0335
3649 1016.4890 2030.9634
3650 678.2984 2031.8734
3651 678.3198 2031.9376
3652 679.3203 2034.9391
3653 1018.4810 2034.9474
3654 680.6780 2039.0122
3655 1020.5173 2039.0200
3656 1020.9457 2039.8768
3657 1020.9562 2039.8978
3658 680.9958 2039.9656
3659 1021.0146 2040.0146
3660 511.0329 2040.1025
3661 1021.4898 2040.9650
3662 681.3293 2040.9661
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