MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3442 947.5095 1893.0044
3443 947.9394 1893.8642
3444 947.9918 1893.9690
3445 632.9513 1895.8321
3446 948.9894 1895.9642
3447 948.9913 1895.9680
3448 475.9577 1899.8017
3449 634.3061 1899.8965
3450 951.4407 1900.8668
3451 951.4507 1900.8868
3452 952.4496 1902.8846
3453 952.9368 1903.8590
3454 953.4333 1904.8520
3455 954.4459 1906.8772
3456 956.4507 1910.8868
3457 637.9922 1910.9548
3458 956.4906 1910.9666
3459 956.9786 1911.9426
3460 638.3222 1911.9448
3461 956.9808 1911.9470
3462 957.4675 1912.9204
3463 958.4494 1914.8842
3464 959.4631 1916.9116
3465 960.4453 1918.8760
3466 961.4521 1920.8896
3467 961.4706 1920.9266
3468 641.3344 1920.9814
3469 961.9990 1921.9834
3470 643.2988 1926.8746
3471 964.4460 1926.8774
3472 964.4741 1926.9336
3473 964.9753 1927.9360
3474 643.9930 1928.9572
3475 966.4529 1930.8912
3476 483.7395 1930.9289
3477 483.7399 1930.9305
3479 967.9510 1933.8874
3480 645.6376 1933.8910
3481 967.9587 1933.9028
3482 484.7374 1934.9205
3483 646.3220 1935.9442
3484 968.9888 1935.9630
3485 646.3367 1935.9883
3486 969.4340 1936.8534
3487 969.4912 1936.9678
3488 969.4927 1936.9708
3489 646.6652 1936.9738
3490 969.5029 1936.9912
3491 646.9980 1937.9722
3493 971.4523 1940.8900
3494 971.4760 1940.9374
3495 647.9940 1940.9602
3496 972.9880 1943.9614
3497 649.3219 1944.9439
3498 975.4521 1948.8896
3499 975.4562 1948.8978
3500 650.9668 1949.8786
3501 650.9705 1949.8897
3502 488.4803 1949.8921
3503 488.4809 1949.8945
3504 976.0140 1950.0134
3505 976.4476 1950.8806
3507 976.4835 1950.9524
3508 976.4851 1950.9556
3509 651.6603 1951.9591
3510 976.9988 1951.9830
3511 976.9990 1951.9834
3512 651.6687 1951.9843
3513 651.6687 1951.9843
3514 977.4769 1952.9392
3515 977.4845 1952.9544
3516 977.4854 1952.9562
3517 977.4968 1952.9790
3518 977.9860 1953.9574
3519 652.3265 1953.9577
3520 652.6395 1954.8967
3521 978.4743 1954.9340
3522 489.9819 1955.8985
3523 980.4485 1958.8824
3524 653.9692 1958.8858
3525 653.9705 1958.8897
3526 980.4552 1958.8958
3527 982.4628 1962.9110
3528 982.4654 1962.9162
3529 982.4752 1962.9358
3532 983.4486 1964.8826
3533 983.4489 1964.8832
3534 983.5219 1965.0292
3536 984.4979 1966.9812
3537 656.9909 1967.9509
3538 984.9839 1967.9532
3540 984.9901 1967.9656
3542 985.4893 1968.9640
3543 657.3299 1968.9679
3544 985.4914 1968.9682
3546 987.9858 1973.9570
3547 659.3209 1974.9409
3548 988.4778 1974.9410
3549 988.4814 1974.9482
3550 989.4805 1976.9464
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