MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3315 605.6086 1813.8040
3316 908.8748 1815.7350
3317 908.9518 1815.8890
3318 909.4597 1816.9048
3319 909.8667 1817.7188
3320 910.4241 1818.8336
3321 910.4423 1818.8700
3322 910.9335 1819.8524
3323 911.3861 1820.7576
3324 607.9291 1820.7655
3328 608.2167 1821.6283
3330 608.3188 1821.9346
3331 912.3964 1822.7782
3333 912.4413 1822.8680
3334 912.9090 1823.8034
3335 913.4297 1824.8448
3336 913.4338 1824.8530
3337 914.4334 1826.8522
3338 916.4824 1830.9502
3339 916.8719 1831.7292
3340 611.6271 1831.8595
3342 917.4379 1832.8612
3343 917.9378 1833.8610
3344 917.9456 1833.8766
3345 459.4828 1833.9021
3346 612.3121 1833.9145
3347 918.4255 1834.8364
3348 918.4355 1834.8564
3349 918.9279 1835.8412
3351 613.2952 1836.8638
3352 919.8839 1837.7532
3353 919.9437 1837.8728
3354 920.3948 1838.7750
3355 920.4513 1838.8880
3356 920.4532 1838.8918
3357 613.9715 1838.8927
3358 614.2999 1839.8779
3361 921.4335 1840.8524
3362 615.2466 1842.7180
3363 922.3784 1842.7422
3365 615.3237 1842.9493
3366 615.9163 1844.7271
3367 923.9447 1845.8748
3368 924.4405 1846.8664
3369 924.4714 1846.9282
3370 924.4721 1846.9296
3371 925.4351 1848.8556
3372 925.4359 1848.8572
3373 925.9459 1849.8772
3374 617.9507 1850.8303
3376 926.4471 1850.8796
3377 926.9149 1851.8152
3379 926.9695 1851.9244
3380 464.2202 1852.8517
3381 618.6270 1852.8592
3382 618.6414 1852.9024
3383 927.4614 1852.9082
3384 927.8250 1853.6354
3386 619.2870 1854.8392
3387 619.3456 1855.0150
3389 620.5782 1858.7128
3390 930.4457 1858.8768
3391 930.4471 1858.8796
3392 620.6365 1858.8877
3393 930.4572 1858.8998
3395 931.9420 1861.8694
3396 621.6318 1861.8736
3400 932.4340 1862.8534
3401 932.4551 1862.8956
3402 932.9337 1863.8528
3403 932.9431 1863.8716
3404 933.4153 1864.8160
3405 933.4313 1864.8480
3407 622.6377 1864.8913
3408 933.4531 1864.8916
3409 934.0504 1866.0862
3410 934.0518 1866.0890
3411 623.0379 1866.0919
3412 934.4416 1866.8686
3413 623.3716 1867.0930
3415 936.4887 1870.9628
3416 624.9848 1871.9326
3420 626.3040 1875.8902
3421 939.4429 1876.8712
3423 939.9291 1877.8436
3424 940.4225 1878.8304
3425 627.6431 1879.9075
3426 627.9706 1880.8900
3427 942.9728 1883.9310
3428 943.4418 1884.8690
3429 630.3137 1887.9193
3430 944.9711 1887.9276
3431 945.4332 1888.8518
3432 945.4443 1888.8740
3433 946.4838 1890.9530
3434 946.4882 1890.9618
3436 631.6301 1891.8685
3437 474.2282 1892.8837
3438 474.2286 1892.8853
3440 947.4987 1892.9828
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