MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3205 868.8909 1735.7672
3206 579.9341 1736.7805
3207 869.3986 1736.7826
3208 869.4304 1736.8462
3209 580.2662 1737.7768
3210 871.4135 1740.8124
3211 871.4904 1740.9662
3212 581.3298 1740.9676
3213 581.3300 1740.9682
3214 871.4926 1740.9706
3215 581.5812 1741.7218
3216 871.8686 1741.7226
3217 871.9209 1741.8272
3218 871.9698 1741.9250
3219 872.3668 1742.7190
3220 872.3691 1742.7236
3222 872.4703 1742.9260
3223 872.8564 1743.6982
3224 582.2413 1743.7021
3225 873.9358 1745.8570
3226 874.4214 1746.8282
3227 874.9152 1747.8158
3228 583.6404 1747.8994
3229 583.6416 1747.9030
3230 875.0035 1747.9924
3231 875.3920 1748.7694
3232 875.4251 1748.8356
3233 875.8924 1749.7702
3236 876.3948 1750.7750
3237 876.3984 1750.7822
3239 585.2636 1752.7690
3242 878.9896 1755.9646
3243 879.8656 1757.7166
3244 879.8831 1757.7516
3245 879.9200 1757.8254
3246 587.2424 1758.7054
3247 880.3638 1758.7130
3249 587.5725 1759.6957
3250 880.8578 1759.7010
3251 880.8582 1759.7018
3252 880.9300 1759.8454
3253 587.6238 1759.8496
3254 881.4781 1760.9416
3255 883.4127 1764.8108
3256 883.4182 1764.8218
3257 883.8947 1765.7748
3258 886.4268 1770.8390
3259 886.4383 1770.8620
3260 886.9485 1771.8824
3262 888.3612 1774.7078
3263 888.4205 1774.8264
3264 888.4409 1774.8672
3265 592.6432 1774.9078
3266 888.9177 1775.8208
3267 888.9369 1775.8592
3268 889.4080 1776.8014
3270 890.4589 1778.9032
3271 445.7362 1778.9157
3272 890.9490 1779.8834
3273 890.9518 1779.8890
3274 594.3136 1779.9190
3275 892.4356 1782.8566
3276 595.3043 1782.8911
3277 595.5598 1783.6576
3278 892.9286 1783.8426
3279 892.9379 1783.8612
3280 894.9837 1787.9528
3281 895.4303 1788.8460
3282 896.4086 1790.8026
3283 896.4166 1790.8186
3284 897.8956 1793.7766
3285 897.9017 1793.7888
3286 598.9419 1793.8039
3287 897.9276 1793.8406
3289 898.9658 1795.9170
3290 599.8941 1796.6605
3291 899.9915 1797.9684
3292 900.3748 1798.7350
3293 600.6047 1798.7923
3294 900.4265 1798.8384
3295 901.6970 1801.3794
3296 901.8688 1801.7230
3297 601.5820 1801.7242
3298 901.9434 1801.8722
3299 902.4313 1802.8480
3300 902.4343 1802.8540
3301 902.4357 1802.8568
3302 902.4423 1802.8700
3303 903.3922 1804.7698
3304 903.3923 1804.7700
3305 602.9326 1805.7760
3306 904.4211 1806.8276
3307 905.4401 1808.8656
3308 604.6328 1810.8766
3309 604.9680 1811.8822
3310 906.9996 1811.9846
3311 907.3206 1812.6266
3312 605.2170 1812.6292
3313 907.4429 1812.8712
3314 605.2979 1812.8719
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