MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4709)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3084 824.4291 1646.8436
3085 824.9044 1647.7942
3087 825.3732 1648.7318
3088 550.5857 1648.7353
3089 825.8815 1649.7484
3090 825.9342 1649.8538
3091 825.9377 1649.8608
3092 826.3674 1650.7202
3093 827.3987 1652.7828
3096 829.9015 1657.7884
3098 831.3900 1660.7654
3099 831.3934 1660.7722
3100 555.2562 1662.7468
3102 832.4015 1662.7884
3103 556.2578 1665.7516
3104 833.8944 1665.7742
3105 834.3660 1666.7174
3107 834.3970 1666.7794
3108 834.4036 1666.7926
3109 834.8582 1667.7018
3110 834.9070 1667.7994
3111 835.4432 1668.8718
3113 558.2626 1671.7660
3114 836.9387 1671.8628
3116 558.2963 1671.8671
3117 837.4376 1672.8606
3118 837.4429 1672.8712
3119 837.8994 1673.7842
3120 838.4218 1674.8290
3122 838.8862 1675.7578
3123 838.8864 1675.7582
3124 840.8869 1679.7592
3125 560.9272 1679.7598
3126 560.9272 1679.7598
3127 841.9619 1681.9092
3128 842.4034 1682.7922
3129 842.4229 1682.8312
3131 842.9036 1683.7926
3132 843.3932 1684.7718
3133 843.4028 1684.7910
3134 843.4117 1684.8088
3135 843.4164 1684.8182
3136 844.9183 1687.8220
3137 844.9334 1687.8522
3138 845.4105 1688.8064
3139 563.9476 1688.8210
3140 845.4302 1688.8458
3141 845.9216 1689.8286
3143 564.2984 1689.8734
3146 846.8787 1691.7428
3147 846.8826 1691.7506
3148 846.8834 1691.7522
3150 848.3784 1694.7422
3151 848.4212 1694.8278
3152 566.2587 1695.7543
3153 566.2590 1695.7552
3155 850.3749 1698.7352
3156 850.4221 1698.8296
3157 850.4236 1698.8326
3158 851.3879 1700.7612
3159 851.4109 1700.8072
3160 568.6177 1702.8313
3161 852.4257 1702.8368
3163 853.4200 1704.8254
3164 853.4295 1704.8444
3165 855.3776 1708.7406
3166 855.4089 1708.8032
3167 855.4186 1708.8226
3168 855.4323 1708.8500
3169 856.3991 1710.7836
3170 856.4036 1710.7926
3171 857.4146 1712.8146
3172 857.4201 1712.8256
3173 572.2419 1713.7039
3174 858.3699 1714.7252
3175 858.4213 1714.8280
3176 858.4275 1714.8404
3177 858.9147 1715.8148
3178 859.4177 1716.8208
3179 859.9470 1717.8794
3180 573.9499 1718.8279
3181 861.8742 1721.7338
3183 575.2481 1722.7225
3184 862.3753 1722.7360
3185 862.8676 1723.7206
3187 863.4113 1724.8080
3188 863.4114 1724.8082
3190 863.9279 1725.8412
3191 576.2976 1725.8710
3192 864.4306 1726.8466
3193 865.4448 1728.8750
3195 577.5649 1729.6729
3196 865.9681 1729.9216
3197 866.9134 1731.8122
3198 867.4153 1732.8160
3199 867.8948 1733.7750
3200 867.8954 1733.7762
3201 868.4052 1734.7958
3202 868.4183 1734.8220
3204 868.4747 1734.9348
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