MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2952 781.9025 1561.7904
2956 781.9226 1561.8306
2960 782.8614 1563.7082
2961 782.8698 1563.7250
2962 782.8718 1563.7290
2965 785.8814 1569.7482
2968 785.9315 1569.8484
2969 786.9139 1571.8132
2970 786.9173 1571.8200
2971 787.3917 1572.7688
2972 787.8946 1573.7746
2973 787.9161 1573.8176
2974 787.9166 1573.8186
2975 788.3674 1574.7202
2976 525.9164 1574.7274
2977 788.3950 1574.7754
2978 526.2436 1575.7090
2979 789.3972 1576.7798
2984 527.2797 1578.8173
2985 791.3914 1580.7682
2986 528.2232 1581.6478
2987 528.2376 1581.6910
2988 791.8810 1581.7474
2989 792.8663 1583.7180
2990 792.8913 1583.7680
2991 793.3906 1584.7666
2992 529.5864 1585.7374
2993 397.4434 1585.7445
2994 793.9241 1585.8336
2995 794.3854 1586.7562
2996 794.9074 1587.8002
2998 794.9120 1587.8094
2999 794.9213 1587.8280
3000 795.3889 1588.7632
3002 530.8829 1589.6269
3003 795.8817 1589.7488
3004 795.9097 1589.8048
3006 795.9149 1589.8152
3007 531.2482 1590.7228
3008 796.9318 1591.8490
3009 797.8736 1593.7326
3010 798.3772 1594.7398
3011 798.3799 1594.7452
3012 798.8855 1595.7564
3013 798.8870 1595.7594
3014 534.2482 1599.7228
3015 800.8693 1599.7240
3016 400.9385 1599.7249
3017 534.2493 1599.7261
3018 400.9390 1599.7269
3019 400.9402 1599.7317
3025 535.2799 1602.8179
3026 802.8460 1603.6774
3027 804.4073 1606.8000
3028 804.8622 1607.7098
3029 805.4073 1608.8000
3030 805.4102 1608.8058
3031 805.8695 1609.7244
3032 805.8704 1609.7262
3033 538.6038 1612.7896
3034 539.5726 1615.6960
3036 539.5747 1615.7023
3037 808.8989 1615.7832
3038 808.9108 1615.8070
3039 808.9211 1615.8276
3040 809.8922 1617.7698
3041 810.3943 1618.7740
3043 540.6077 1618.8013
3044 810.4119 1618.8092
3045 810.4330 1618.8514
3046 810.4412 1618.8678
3048 811.4109 1620.8072
3049 811.9066 1621.7986
3050 812.3667 1622.7188
3051 812.3677 1622.7208
3052 812.4489 1622.8832
3053 542.2485 1623.7237
3054 812.9099 1623.8052
3055 542.9229 1625.7469
3056 814.8842 1627.7538
3059 814.8900 1627.7654
3060 816.4065 1630.7984
3062 816.4144 1630.8142
3064 816.9047 1631.7948
3065 816.9190 1631.8234
3066 816.9384 1631.8622
3067 817.4217 1632.8288
3069 817.9116 1633.8086
3070 817.9125 1633.8104
3071 818.3850 1634.7554
3072 818.4112 1634.8078
3074 819.8551 1637.6956
3075 547.5958 1639.7656
3076 547.5979 1639.7719
3078 821.9065 1641.7984
3079 548.5806 1642.7200
3080 823.3594 1644.7042
3081 549.2465 1644.7177
3082 824.4219 1646.8292
3083 824.4277 1646.8408
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