MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2808 737.8501 1473.6856
2810 492.2738 1473.7996
2811 737.9075 1473.8004
2812 737.9081 1473.8016
2813 492.2747 1473.8023
2815 739.3685 1476.7224
2816 739.8610 1477.7074
2817 739.8631 1477.7116
2819 741.3799 1480.7452
2820 741.8349 1481.6552
2821 495.5805 1483.7197
2822 495.5807 1483.7203
2824 495.8967 1484.6683
2825 743.3787 1484.7428
2826 743.8674 1485.7202
2827 743.8759 1485.7372
2828 744.3425 1486.6704
2829 745.3627 1488.7108
2830 745.8513 1489.6880
2834 497.5764 1489.7074
2835 746.8566 1491.6986
2836 746.8726 1491.7306
2838 747.8445 1493.6744
2839 747.8480 1493.6814
2840 748.3574 1494.7002
2841 748.8494 1495.6842
2842 499.6006 1495.7800
2846 500.9118 1499.7136
2848 500.9127 1499.7163
2849 500.9495 1499.8267
2851 751.8715 1501.7284
2852 751.8717 1501.7288
2854 752.8790 1503.7434
2855 752.8953 1503.7760
2856 753.3985 1504.7824
2857 753.8572 1505.6998
2858 753.8583 1505.7020
2859 754.3165 1506.6184
2860 754.3724 1506.7302
2861 755.3270 1508.6394
2862 755.8320 1509.6494
2863 504.2256 1509.6550
2867 756.8792 1511.7438
2868 756.8794 1511.7442
2869 757.3738 1512.7330
2870 757.3739 1512.7332
2871 505.5617 1513.6633
2875 506.2485 1515.7237
2876 759.8599 1517.7052
2877 506.9093 1517.7061
2878 759.8696 1517.7246
2879 759.8849 1517.7552
2880 760.8610 1519.7074
2881 760.8779 1519.7412
2882 761.3391 1520.6636
2883 761.8459 1521.6772
2884 762.3622 1522.7098
2885 508.9250 1523.7532
2886 763.3591 1524.7036
2887 509.5847 1525.7323
2889 382.9208 1527.6541
2890 765.3701 1528.7256
2892 510.5846 1528.7320
2893 765.3746 1528.7346
2896 765.9117 1529.8088
2897 767.3693 1532.7240
2898 767.3734 1532.7322
2900 767.4039 1532.7932
2901 767.4048 1532.7950
2902 512.2410 1533.7012
2903 512.2410 1533.7012
2904 768.3618 1534.7090
2905 768.3641 1534.7136
2906 768.4022 1534.7898
2907 512.9014 1535.6824
2908 769.3365 1536.6584
2909 769.3546 1536.6946
2911 513.5784 1537.7134
2914 769.8884 1537.7622
2915 514.2316 1539.6730
2916 514.5892 1540.7458
2917 515.2135 1542.6187
2918 772.8722 1543.7298
2919 773.3891 1544.7636
2920 773.8116 1545.6086
2921 773.8161 1545.6176
2927 774.3709 1546.7272
2929 774.8517 1547.6888
2930 775.3678 1548.7210
2932 775.3998 1548.7850
2935 777.8843 1553.7540
2937 778.3817 1554.7488
2938 778.3868 1554.7590
2939 778.8350 1555.6554
2940 778.8752 1555.7358
2941 778.9189 1555.8232
2942 779.6718 1557.3290
2944 780.3849 1558.7552
2945 780.3853 1558.7560
2949 781.8990 1561.7834
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