MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2674 681.8138 1361.6130
2675 682.3337 1362.6528
2676 682.3738 1362.7330
2677 682.3742 1362.7338
2678 455.5393 1363.5961
2679 682.8076 1363.6006
2680 683.2981 1364.5816
2681 684.3364 1366.6582
2682 456.5822 1366.7248
2684 685.3452 1368.6758
2686 688.8450 1375.6754
2688 690.3251 1378.6356
2689 690.3499 1378.6852
2690 690.3540 1378.6934
2692 691.3339 1380.6532
2693 691.8119 1381.6092
2694 692.8553 1383.6960
2696 693.3412 1384.6678
2697 693.3426 1384.6706
2698 462.5699 1384.6879
2700 695.3464 1388.6782
2701 695.8362 1389.6578
2703 464.5465 1390.6177
2704 696.3477 1390.6808
2705 697.8580 1393.7014
2706 699.8762 1397.7378
2707 700.3731 1398.7316
2708 700.3742 1398.7338
2710 701.2880 1400.5614
2711 701.3405 1400.6664
2712 701.7849 1401.5552
2713 702.8615 1403.7084
2715 703.3605 1404.7064
2716 703.8351 1405.6556
2719 707.3488 1412.6830
2721 708.3892 1414.7638
2722 709.3439 1416.6732
2723 710.8575 1419.7004
2724 710.8588 1419.7030
2725 710.8592 1419.7038
2727 711.8762 1421.7378
2728 711.8783 1421.7420
2729 712.3270 1422.6394
2730 713.3597 1424.7048
2731 713.8577 1425.7008
2732 714.3246 1426.6346
2733 715.8335 1429.6524
2735 715.8696 1429.7246
2736 715.8717 1429.7288
2737 716.3865 1430.7584
2738 716.3887 1430.7628
2742 717.8911 1433.7676
2743 717.8920 1433.7694
2744 717.8924 1433.7702
2745 478.9309 1433.7709
2746 479.2332 1434.6778
2747 718.3464 1434.6782
2748 718.3481 1434.6816
2749 719.3345 1436.6544
2751 720.8652 1439.7158
2752 721.3203 1440.6260
2753 722.3553 1442.6960
2754 722.8788 1443.7430
2756 723.8611 1445.7076
2758 482.9136 1445.7190
2759 723.8673 1445.7200
2761 723.8690 1445.7234
2762 723.8704 1445.7262
2764 723.8716 1445.7286
2765 723.8723 1445.7300
2766 723.8729 1445.7312
2767 723.8735 1445.7324
2768 723.8746 1445.7346
2769 723.8755 1445.7364
2770 723.8765 1445.7384
2771 723.8766 1445.7386
2772 723.8784 1445.7422
2773 724.3754 1446.7362
2777 725.8379 1449.6612
2778 725.8463 1449.6780
2779 725.8792 1449.7438
2780 726.3705 1450.7264
2781 726.3716 1450.7286
2782 726.8258 1451.6370
2784 728.8647 1455.7148
2786 486.8906 1457.6500
2787 487.2394 1458.6964
2789 730.3859 1458.7572
2790 730.8738 1459.7330
2792 730.8762 1459.7378
2793 730.8988 1459.7830
2794 730.9146 1459.8146
2795 487.9362 1460.7868
2796 731.4016 1460.7886
2797 488.2353 1461.6841
2798 731.8542 1461.6938
2799 731.8616 1461.7086
2801 490.2078 1467.6016
2806 736.3514 1470.6882
2807 736.3690 1470.7234
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