MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 4 |
| MS data file | : | PRT1245_Other_P1_B12.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:22:18 GMT |
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the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,772 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 1902 |
1048.5178 | 1047.5105 | 1047.5196 | -8.67 | 1 | 6 | 0.37 | 1Score > 22 indicates identity Score > 14 indicates homology |
VKQLSSNNR + 3 Deamidated (NQ) |
| 2254 |
567.2898 | 1132.5650 | 1132.5798 | -13.0 | 1 | 6 | 1 | 1Score > 23 indicates identity Score > 18 indicates homology |
LDPKTAETMK |
| 1197 |
800.3795 | 799.3722 | 799.3864 | -17.8 | 0 | 6 | 0.71 | 1Score > 17 indicates identity |
YNYAIR + Deamidated (NQ) |
| 969 |
322.6721 | 643.3296 | 643.3289 | 1.11 | 0 | 6 | 0.91 | 1Score > 18 indicates identity |
LGSPDR |
| 1567 |
950.4695 | 949.4622 | 949.4552 | 7.38 | 1 | 5 | 0.87 | 1Score > 22 indicates identity Score > 17 indicates homology |
HMTRSFR + Oxidation (M) |
| 2051 |
542.7861 | 1083.5576 | 1083.5448 | 11.9 | 0 | 5 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
PINQPSGQIK + 3 Deamidated (NQ) |
| 2814 |
738.3923 | 1474.7700 | 1474.7515 | 12.6 | 1 | 5 | 0.35 | 1Score > 22 indicates identity Score > 13 indicates homology |
VSQLESADKQLEK + Deamidated (NQ) |
| 3843 |
1058.4988 | 2114.9830 | 2114.9646 | 8.71 | 1 | 5 | 1 | 1Score > 21 indicates identity Score > 18 indicates homology |
VQTAVTMRQVSASMGQVCK + 3 Deamidated (NQ); 2 Oxidation (M) |
| 1323 |
431.2146 | 860.4146 | 860.3988 | 18.4 | 0 | 5 | 0.63 | 1Score > 22 indicates identity Score > 16 indicates homology |
QLQDNSR + Deamidated (NQ) |
| 3559 |
661.3401 | 1980.9985 | 1980.9792 | 9.71 | 1 | 5 | 0.72 | 1Score > 22 indicates identity Score > 16 indicates homology |
NLDTFLNQLRDFLNEK + 2 Deamidated (NQ) |
| 2471 |
592.7633 | 1183.5120 | 1183.5292 | -14.5 | 0 | 5 | 1.2 | 1Score > 19 indicates identity |
QSQFNLMSGR + Deamidated (NQ); Oxidation (M) |
| 2272 |
569.7794 | 1137.5442 | 1137.5666 | -19.6 | 1 | 5 | 0.35 | 1Score > 23 indicates identity Score > 13 indicates homology |
AGDKYSIEQK |
| 1084 |
371.1805 | 740.3464 | 740.3453 | 1.56 | 0 | 5 | 0.84 | 1Score > 17 indicates identity |
HNNDLK + Deamidated (NQ) |
| 1129 |
764.3578 | 763.3505 | 763.3534 | -3.82 | 1 | 5 | 1 | 1Score > 21 indicates identity Score > 18 indicates homology |
CVKDDK |
| 993 |
667.3527 | 666.3454 | 666.3561 | -16.1 | 0 | 5 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
LQNHR |
| 2326 |
575.2880 | 1148.5614 | 1148.5713 | -8.58 | 0 | 5 | 0.95 | 1Score > 22 indicates identity Score > 18 indicates homology |
NLPSLEANYK + Deamidated (NQ) |
| 1087 |
742.3768 | 741.3695 | 741.3592 | 13.9 | 0 | 5 | 1.1 | 1Score > 18 indicates identity |
CAQVHK |
| 2284 |
1141.5608 | 1140.5535 | 1140.5346 | 16.6 | 1 | 5 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
AEPRHMQQK + Deamidated (NQ); Oxidation (M) |
| 1525 |
939.4655 | 938.4582 | 938.4709 | -13.5 | 0 | 5 | 0.7 | 1Score > 20 indicates identity Score > 16 indicates homology |
NVTDYLSK |
| 2376 |
581.7937 | 1161.5728 | 1161.5513 | 18.5 | 0 | 5 | 1 | 1Score > 23 indicates identity Score > 18 indicates homology |
GLLGDSENETK |
| 2662 |
674.3314 | 1346.6482 | 1346.6612 | -9.64 | 1 | 5 | 1 | 1Score > 22 indicates identity Score > 18 indicates homology |
QQNIEDMLRGK + Oxidation (M) |
| 4079 |
764.0577 | 2289.1513 | 2289.1207 | 13.4 | 1 | 5 | 1 | 1Score > 22 indicates identity Score > 18 indicates homology |
MMNELLQKLPTCYYQTLK + Oxidation (M) |
| 3685 |
685.6877 | 2054.0413 | 2054.0168 | 11.9 | 0 | 5 | 1 | 1Score > 21 indicates identity Score > 18 indicates homology |
ETNPGTDVTVSSVESVLAHL |
| 1003 |
337.1797 | 672.3448 | 672.3555 | -15.8 | 1 | 5 | 1.3 | 1Score > 19 indicates identity |
EPGKSR |
| 1685 |
494.2562 | 986.4978 | 986.5145 | -16.8 | 1 | 5 | 0.45 | 1Score > 21 indicates identity Score > 14 indicates homology |
QQRQNAIK + 2 Deamidated (NQ) |
| 2564 |
626.8153 | 1251.6160 | 1251.6064 | 7.75 | 1 | 5 | 1 | 1Score > 24 indicates identity Score > 18 indicates homology |
MSLKQMQSQR + Oxidation (M) |
| 2933 |
776.8478 | 1551.6810 | 1551.6657 | 9.88 | 0 | 5 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
NTNIGGMIAECAER + Deamidated (NQ); Oxidation (M) |
| 1374 |
442.7283 | 883.4420 | 883.4399 | 2.40 | 1 | 5 | 1.6 | 1Score > 19 indicates identity |
KDIDEHK |
| 1400 |
448.2375 | 894.4604 | 894.4672 | -7.51 | 0 | 5 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
QPGHLTSR |
| 1296 |
427.2305 | 852.4464 | 852.4527 | -7.39 | 1 | 5 | 1.5 | 1Score > 19 indicates identity |
KIASFCK |
| 1022 |
682.3641 | 681.3568 | 681.3558 | 1.50 | 0 | 5 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
GAEIHR |
| 1531 |
470.7415 | 939.4684 | 939.4709 | -2.56 | 0 | 5 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
VERPMHR + Oxidation (M) |
| 3995 |
1104.0558 | 2206.0970 | 2206.0708 | 11.9 | 0 | 5 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
DKPYFDAEHVIHISHGWR |
| 1045 |
699.3474 | 698.3401 | 698.3282 | 17.1 | 0 | 5 | 0.89 | 1Score > 17 indicates identity |
NCLHR |
| 2393 |
583.2842 | 1164.5538 | 1164.5411 | 10.9 | 0 | 5 | 0.64 | 1Score > 22 indicates identity Score > 15 indicates homology |
QVNFNQETGK + Deamidated (NQ) |
| 1422 |
451.7339 | 901.4532 | 901.4505 | 3.07 | 0 | 5 | 0.91 | 1Score > 22 indicates identity Score > 17 indicates homology |
ELNNSTPK |
| 977 |
655.3433 | 654.3360 | 654.3489 | -19.7 | 0 | 5 | 1.1 | 1Score > 18 indicates identity |
GLFYR |
| 3189 |
576.2763 | 1725.8071 | 1725.7933 | 7.98 | 0 | 5 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
YMEHFAQLDAQFAR |
| 1373 |
442.7280 | 883.4414 | 883.4399 | 1.72 | 1 | 5 | 1.6 | 1Score > 19 indicates identity |
KDIDEHK |
| 1202 |
400.7178 | 799.4210 | 799.4301 | -11.3 | 0 | 5 | 1.3 | 1Score > 18 indicates identity |
LLGGGGGGGR |
| 1723 |
997.4601 | 996.4528 | 996.4625 | -9.67 | 0 | 5 | 0.83 | 1Score > 20 indicates identity Score > 16 indicates homology |
DNPPNGEVR |
| 2064 |
544.2855 | 1086.5564 | 1086.5709 | -13.3 | 0 | 5 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
EHTIIAEFK |
| 1453 |
457.7338 | 913.4530 | 913.4366 | 18.0 | 1 | 5 | 1.5 | 1Score > 19 indicates identity |
RQNEDPR |
| 2520 |
604.3094 | 1206.6042 | 1206.6026 | 1.34 | 1 | 5 | 1 | 1Score > 23 indicates identity Score > 17 indicates homology |
ILSMRNSENK + Oxidation (M) |
| 1013 |
678.3224 | 677.3151 | 677.3166 | -2.23 | 1 | 5 | 0.53 | 1Score > 22 indicates identity Score > 14 indicates homology |
KDMQR + Deamidated (NQ) |
| 1569 |
475.7389 | 949.4632 | 949.4651 | -1.95 | 0 | 5 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
VASSAVMNR + Oxidation (M) |
| 2168 |
1111.5110 | 1110.5037 | 1110.5240 | -18.2 | 1 | 5 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
IERNMYNR + Oxidation (M) |
| 2516 |
602.8030 | 1203.5914 | 1203.6104 | -15.7 | 1 | 5 | 0.38 | 1Score > 22 indicates identity Score > 13 indicates homology |
GPLKGCLNMSK |
| 2241 |
565.8224 | 1129.6302 | 1129.6091 | 18.7 | 1 | 5 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
KSPNSVSAVNK |
| 1042 |
349.1981 | 696.3816 | 696.3919 | -14.7 | 0 | 5 | 0.59 | 1Score > 15 indicates identity |
SVNHLK |
| 1948 |
1058.4954 | 1057.4881 | 1057.4862 | 1.78 | 1 | 5 | 1.4 | 1Score > 19 indicates identity |
MYDTTQKR + Oxidation (M) |
| 1973 |
532.7610 | 1063.5074 | 1063.5121 | -4.33 | 0 | 5 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
MGPSYVSAPR |
| 4438 |
956.4920 | 2866.4542 | 2866.4462 | 2.77 | 1 | 5 | 0.64 | 1Score > 20 indicates identity Score > 15 indicates homology |
NGLEFIVAFLGATMDAKIGAPLNPNYK + 3 Deamidated (NQ) |
| 2934 |
776.8959 | 1551.7772 | 1551.7967 | -12.5 | 0 | 5 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
LLVELVSSYNQCK |
| 2232 |
564.3015 | 1126.5884 | 1126.5692 | 17.1 | 1 | 5 | 1.7 | 1Score > 19 indicates identity |
YNVIKSMQK + Deamidated (NQ); Oxidation (M) |
| 1052 |
705.3206 | 704.3133 | 704.3089 | 6.24 | 0 | 5 | 1.2 | 1Score > 18 indicates identity |
GNSGVDR + Deamidated (NQ) |
| 2099 |
548.2574 | 1094.5002 | 1094.5026 | -2.17 | 0 | 4 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
GSTANMSLGGGK + Oxidation (M) |
| 1843 |
1031.5031 | 1030.4958 | 1030.5043 | -8.24 | 0 | 4 | 1 | 1Score > 23 indicates identity Score > 17 indicates homology |
SIPGASSQQR + Deamidated (NQ) |
| 1028 |
343.1980 | 684.3814 | 684.3918 | -15.2 | 0 | 4 | 1.2 | 1Score > 18 indicates identity |
VNRPAK + Deamidated (NQ) |
| 2149 |
553.2737 | 1104.5328 | 1104.5485 | -14.1 | 0 | 4 | 1 | 1Score > 23 indicates identity Score > 17 indicates homology |
AMALDAQLQK + Deamidated (NQ); Oxidation (M) |
| 1496 |
465.7311 | 929.4476 | 929.4389 | 9.44 | 1 | 4 | 1.5 | 1Score > 19 indicates identity |
QHMQKNK + Deamidated (NQ); Oxidation (M) |
| 880 |
544.3095 | 543.3022 | 543.3017 | 1.05 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
KGPDK |
| 1005 |
673.3525 | 672.3452 | 672.3555 | -15.3 | 0 | 4 | 1.5 | 1Score > 19 indicates identity |
VAVGNGR + Deamidated (NQ) |
| 1428 |
453.7199 | 905.4252 | 905.4276 | -2.65 | 0 | 4 | 0.93 | 1Score > 23 indicates identity Score > 17 indicates homology |
QNEQIMK + Oxidation (M) |
| 1809 |
511.7688 | 1021.5230 | 1021.5226 | 0.42 | 0 | 4 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
MAGATSSIIR + Oxidation (M) |
| 956 |
319.1872 | 636.3598 | 636.3707 | -17.1 | 0 | 4 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
QHKPK |
| 2620 |
652.3025 | 1302.5904 | 1302.6027 | -9.38 | 0 | 4 | 0.8 | 1Score > 21 indicates identity Score > 16 indicates homology |
IGYCIHPGSNGK + Deamidated (NQ) |
| 4362 |
898.4448 | 2692.3126 | 2692.3384 | -9.59 | 0 | 4 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
TAQTINDFNYGEQLPLIILANWR + 3 Deamidated (NQ) |
| 3023 |
801.9212 | 1601.8278 | 1601.8382 | -6.44 | 1 | 4 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
STVPIIIRCCIDR |
| 3545 |
657.3301 | 1968.9685 | 1968.9350 | 17.0 | 0 | 4 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
MSNTEELIQNSIGFLQK + 2 Deamidated (NQ); Oxidation (M) |
| 1061 |
361.7015 | 721.3884 | 721.3759 | 17.4 | 0 | 4 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
ALSQFR + Deamidated (NQ) |
| 2785 |
728.8864 | 1455.7582 | 1455.7681 | -6.80 | 1 | 4 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
KTTTSPINIPGGNR + Deamidated (NQ) |
| 874 |
529.2993 | 528.2920 | 528.3020 | -18.9 | 0 | 4 | 1.5 | 1Score > 18 indicates identity |
AVPSR |
| 1384 |
891.4343 | 890.4270 | 890.4093 | 19.9 | 1 | 4 | 0.7 | 1Score > 23 indicates identity Score > 15 indicates homology |
EAQNKGSR + 2 Deamidated (NQ) |
| 1642 |
485.7468 | 969.4790 | 969.4767 | 2.39 | 1 | 4 | 1.8 | 1Score > 19 indicates identity |
KDTTGSSFK |
| 1133 |
767.3675 | 766.3602 | 766.3531 | 9.31 | 1 | 4 | 0.8 | 1Score > 20 indicates identity Score > 16 indicates homology |
KDMQTK + Deamidated (NQ); Oxidation (M) |
| 2369 |
580.7853 | 1159.5560 | 1159.5770 | -18.1 | 0 | 4 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
MVPTMFPPPK + Oxidation (M) |
| 1632 |
968.4734 | 967.4661 | 967.4545 | 12.0 | 1 | 4 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
FMEQKNR + Oxidation (M) |
| 1930 |
1054.4812 | 1053.4739 | 1053.4839 | -9.49 | 1 | 4 | 0.59 | 1Score > 20 indicates identity Score > 14 indicates homology |
TDNQYTRR + Deamidated (NQ) |
| 1143 |
386.2253 | 770.4360 | 770.4286 | 9.60 | 0 | 4 | 1.4 | 1Score > 18 indicates identity |
GGADVKPK |
| 3364 |
922.4781 | 1842.9416 | 1842.9476 | -3.22 | 0 | 4 | 0.44 | 1Score > 21 indicates identity Score > 13 indicates homology |
LSPGDDLISFQPVSVNR |
| 2028 |
540.7524 | 1079.4902 | 1079.4917 | -1.36 | 1 | 4 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
KNDDGSMVAK + Oxidation (M) |
| 1550 |
947.4509 | 946.4436 | 946.4443 | -0.74 | 1 | 4 | 0.42 | 1Score > 22 indicates identity Score > 13 indicates homology |
GMGFHRDK |
| 1153 |
779.4164 | 778.4091 | 778.3973 | 15.2 | 1 | 4 | 0.73 | 1Score > 22 indicates identity Score > 15 indicates homology |
KEAFER |
| 2953 |
781.9047 | 1561.7948 | 1561.8239 | -18.6 | 0 | 4 | 1 | 1Score > 23 indicates identity Score > 17 indicates homology |
ATLSTLFQELNVPK + 2 Deamidated (NQ) |
| 2832 |
745.8604 | 1489.7062 | 1489.6783 | 18.7 | 0 | 4 | 0.53 | 1Score > 22 indicates identity Score > 14 indicates homology |
AVEALNDSELNGEK + 2 Deamidated (NQ) |
| 3836 |
705.3386 | 2112.9940 | 2113.0224 | -13.4 | 1 | 4 | 0.51 | 1Score > 22 indicates identity Score > 14 indicates homology |
IDMYKEQGFGNLPICIAK + Deamidated (NQ); Oxidation (M) |
| 3086 |
824.9082 | 1647.8018 | 1647.8146 | -7.75 | 0 | 4 | 1 | 1Score > 23 indicates identity Score > 16 indicates homology |
ILQMGAIMNLGMPSR + Deamidated (NQ); Oxidation (M) |
| 1722 |
499.2332 | 996.4518 | 996.4552 | -3.41 | 0 | 4 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
VSSWENFK + Deamidated (NQ) |
| 2554 |
414.5512 | 1240.6318 | 1240.6339 | -1.75 | 0 | 4 | 0.7 | 1Score > 23 indicates identity Score > 15 indicates homology |
YVLYGVSTPNK + Deamidated (NQ) |
| 2580 |
635.8052 | 1269.5958 | 1269.5911 | 3.77 | 1 | 4 | 1 | 1Score > 21 indicates identity Score > 16 indicates homology |
STAKIDFNNMK + 2 Deamidated (NQ) |
| 2541 |
610.8275 | 1219.6404 | 1219.6448 | -3.59 | 0 | 4 | 1 | 1Score > 23 indicates identity Score > 16 indicates homology |
AVGNIVNEIYK + Deamidated (NQ) |
| 1427 |
905.4468 | 904.4395 | 904.4501 | -11.7 | 1 | 4 | 1 | 1Score > 23 indicates identity Score > 16 indicates homology |
SIDNKAEK + Deamidated (NQ) |
| 3068 |
817.4252 | 1632.8358 | 1632.8215 | 8.78 | 0 | 4 | 0.67 | 1Score > 22 indicates identity Score > 15 indicates homology |
DSPLSCLLLCTGVAK |
| 1248 |
413.7066 | 825.3986 | 825.4021 | -4.18 | 0 | 4 | 1.7 | 1Score > 19 indicates identity |
QIDFFR + Deamidated (NQ) |
| 2622 |
655.3245 | 1308.6344 | 1308.6323 | 1.63 | 0 | 4 | 1 | 1Score > 22 indicates identity Score > 16 indicates homology |
ANLHGYGNPNPR |
| 3112 |
835.4945 | 1668.9744 | 1668.9814 | -4.18 | 0 | 4 | 0.41 | 1Score > 13 indicates identity |
ELVILGTGWGAISLLK |
| 1249 |
827.4078 | 826.4005 | 826.4120 | -13.8 | 0 | 4 | 1.7 | 1Score > 19 indicates identity |
AVPCQPR |
| 2106 |
1096.5295 | 1095.5222 | 1095.5271 | -4.41 | 0 | 4 | 1 | 1Score > 22 indicates identity Score > 16 indicates homology |
ISDFVCLDK |
| 1278 |
421.7601 | 841.5056 | 841.5021 | 4.17 | 0 | 4 | 1.7 | 1Score > 19 indicates identity |
GNVIILGR + Deamidated (NQ) |
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