MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2530 607.7874 1213.5602
2531 608.7802 1215.5458
2532 608.7802 1215.5458
2533 608.8221 1215.6296
2534 609.2845 1216.5544
2535 609.2975 1216.5804
2536 609.7892 1217.5638
2537 609.8107 1217.6068
2538 610.2938 1218.5730
2539 610.3024 1218.5902
2540 610.7877 1219.5608
2543 611.8296 1221.6446
2544 611.8322 1221.6498
2545 615.7842 1229.5538
2546 615.7843 1229.5540
2548 618.7783 1235.5420
2550 618.8149 1235.6152
2551 620.3244 1238.6342
2552 620.7963 1239.5780
2553 620.8186 1239.6226
2555 621.3239 1240.6332
2556 621.8024 1241.5902
2557 623.3371 1244.6596
2558 623.7817 1245.5488
2559 625.3124 1248.6102
2560 625.3320 1248.6494
2565 628.3074 1254.6002
2566 628.3111 1254.6076
2567 419.8833 1256.6281
2569 629.8002 1257.5858
2570 629.8023 1257.5900
2571 629.8083 1257.6020
2573 631.3191 1260.6236
2574 631.3200 1260.6254
2575 631.3322 1260.6498
2576 631.3330 1260.6514
2577 632.8201 1263.6256
2581 635.8329 1269.6512
2582 636.2980 1270.5814
2585 636.3093 1270.6040
2586 636.3242 1270.6338
2588 638.3217 1274.6288
2589 638.7940 1275.5734
2590 638.8338 1275.6530
2591 638.8350 1275.6554
2592 638.8392 1275.6638
2594 639.3292 1276.6438
2595 639.3301 1276.6456
2596 640.2955 1278.5764
2597 640.3000 1278.5854
2598 640.8091 1279.6036
2600 641.3116 1280.6086
2601 643.3339 1284.6532
2603 644.2961 1286.5776
2604 644.3001 1286.5856
2606 645.2979 1288.5812
2607 430.8778 1289.6116
2608 647.3272 1292.6398
2609 647.8557 1293.6968
2611 649.8215 1297.6284
2612 649.8244 1297.6342
2613 649.8254 1297.6362
2614 651.3279 1300.6412
2615 651.3300 1300.6454
2616 651.8083 1301.6020
2621 652.3370 1302.6594
2625 656.8331 1311.6516
2628 438.8742 1313.6008
2629 658.3115 1314.6084
2630 659.2901 1316.5656
2632 659.8293 1317.6440
2633 659.8465 1317.6784
2635 660.3781 1318.7416
2636 662.8400 1323.6654
2637 662.8408 1323.6670
2638 663.3024 1324.5902
2639 663.3088 1324.6030
2640 664.8116 1327.6086
2643 664.8317 1327.6488
2644 664.8796 1327.7446
2645 664.8799 1327.7452
2646 665.3781 1328.7416
2647 666.8314 1331.6482
2648 666.8324 1331.6502
2649 667.3307 1332.6468
2650 667.3433 1332.6720
2651 667.3448 1332.6750
2652 667.3453 1332.6760
2656 669.3474 1336.6802
2657 669.8584 1337.7022
2658 669.8586 1337.7026
2659 447.8857 1340.6353
2661 672.8278 1343.6410
2663 450.2079 1347.6019
2664 674.8110 1347.6074
2665 674.8287 1347.6428
2666 674.8292 1347.6438
2667 675.3077 1348.6008
2671 451.8985 1352.6737
2672 677.3449 1352.6752
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