MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2404 1168.5457 1167.5384
2405 1168.5502 1167.5429
2406 1168.5536 1167.5463
2407 1168.5541 1167.5468
2408 1168.5559 1167.5486
2409 1169.0549 1168.0476
2410 1169.5493 1168.5420
2411 1169.5508 1168.5435
2412 1169.5529 1168.5456
2413 1170.5458 1169.5385
2414 1170.5497 1169.5424
2415 585.7821 1169.5496
2416 1170.5575 1169.5502
2417 1170.5649 1169.5576
2418 1170.5675 1169.5602
2419 1170.5680 1169.5607
2420 1171.5125 1170.5052
2421 586.2716 1170.5286
2422 1171.5443 1170.5370
2423 1171.5485 1170.5412
2424 586.2831 1170.5516
2425 1171.5681 1170.5608
2426 586.7636 1171.5126
2427 1172.5518 1171.5445
2428 1172.5634 1171.5561
2429 1173.5558 1172.5485
2431 1174.5496 1173.5423
2432 1174.5535 1173.5462
2433 1175.5604 1174.5531
2434 1175.5618 1174.5545
2435 1175.5692 1174.5619
2436 1175.5737 1174.5664
2437 588.7812 1175.5478
2438 1176.5553 1175.5480
2439 588.7817 1175.5488
2441 1176.5740 1175.5667
2442 588.8069 1175.5992
2443 588.8231 1175.6316
2444 588.8237 1175.6328
2445 1177.5422 1176.5349
2446 1177.5568 1176.5495
2447 1177.5626 1176.5553
2448 589.3033 1176.5920
2449 1178.5563 1177.5490
2450 589.7943 1177.5740
2455 1179.5674 1178.5601
2458 1180.5548 1179.5475
2460 1180.5587 1179.5514
2461 1180.5651 1179.5578
2462 591.2753 1180.5360
2463 591.2811 1180.5476
2464 1181.5736 1180.5663
2468 591.8123 1181.6100
2469 1183.5581 1182.5508
2470 1183.5592 1182.5519
2472 1184.5521 1183.5448
2473 1184.5647 1183.5574
2474 592.7873 1183.5600
2475 1184.5709 1183.5636
2476 1184.5713 1183.5640
2477 1184.5718 1183.5645
2478 592.7902 1183.5658
2479 1185.5536 1184.5463
2480 1185.5547 1184.5474
2481 1185.5637 1184.5564
2482 593.7812 1185.5478
2483 1186.5677 1185.5604
2484 1187.5549 1186.5476
2485 1187.5763 1186.5690
2486 1188.5676 1187.5603
2487 1189.5632 1188.5559
2488 1189.5654 1188.5581
2489 1189.5767 1188.5694
2491 1190.5597 1189.5524
2492 1191.5620 1190.5547
2493 1192.5652 1191.5579
2495 596.8237 1191.6328
2496 1193.5642 1192.5569
2497 597.3004 1192.5862
2498 597.3224 1192.6302
2500 1194.5552 1193.5479
2501 597.7932 1193.5718
2502 598.2871 1194.5596
2503 1196.5642 1195.5569
2504 1197.5508 1196.5435
2505 1197.5602 1196.5529
2506 599.3016 1196.5886
2509 600.7823 1199.5500
2510 600.7834 1199.5522
2511 600.7847 1199.5548
2512 600.8630 1199.7114
2513 601.2984 1200.5822
2514 601.2989 1200.5832
2518 603.7923 1205.5700
2519 603.7971 1205.5796
2521 604.8199 1207.6252
2522 604.8210 1207.6274
2523 604.8222 1207.6298
2524 606.7839 1211.5532
2529 607.7870 1213.5594
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