MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2287 571.7616 1141.5086
2288 1142.5164 1141.5091
2289 1142.5178 1141.5105
2291 1142.5354 1141.5281
2292 1142.5374 1141.5301
2293 381.8460 1142.5162
2294 1143.5482 1142.5409
2295 1143.5542 1142.5469
2296 1144.5334 1143.5261
2297 1144.5356 1143.5283
2298 572.7960 1143.5774
2299 572.7972 1143.5798
2300 572.7980 1143.5814
2301 1145.5476 1144.5403
2302 1145.5482 1144.5409
2306 1146.5424 1145.5351
2307 1146.5491 1145.5418
2308 1146.5553 1145.5480
2309 1146.5621 1145.5548
2310 573.8185 1145.6224
2311 1147.5382 1146.5309
2312 1147.5491 1146.5418
2314 1148.5427 1147.5354
2315 1148.5476 1147.5403
2317 1148.5653 1147.5580
2319 1149.5376 1148.5303
2320 1149.5404 1148.5331
2321 575.2812 1148.5478
2322 575.2812 1148.5478
2323 1149.5562 1148.5489
2324 1149.5674 1148.5601
2325 575.2875 1148.5604
2327 1150.5455 1149.5382
2328 1150.5455 1149.5382
2329 1150.5676 1149.5603
2330 1151.5472 1150.5399
2331 1151.5504 1150.5431
2332 1151.5603 1150.5530
2333 1151.5734 1150.5661
2334 576.7679 1151.5212
2336 1152.5397 1151.5324
2337 577.2594 1152.5042
2338 1153.5364 1152.5291
2339 1153.5499 1152.5426
2340 1153.5530 1152.5457
2341 1153.5618 1152.5545
2342 1154.5433 1153.5360
2343 1154.5457 1153.5384
2344 1154.5487 1153.5414
2345 577.7846 1153.5546
2346 1154.5649 1153.5576
2347 577.7864 1153.5582
2349 578.2740 1154.5334
2350 1156.5468 1155.5395
2351 1156.5486 1155.5413
2352 1156.5623 1155.5550
2353 1157.5376 1156.5303
2354 579.2866 1156.5586
2355 1158.5103 1157.5030
2356 579.7597 1157.5048
2357 579.7601 1157.5056
2358 1158.5363 1157.5290
2359 1158.5367 1157.5294
2360 1158.5604 1157.5531
2361 1159.5508 1158.5435
2362 1159.5525 1158.5452
2363 580.7654 1159.5162
2364 1160.5242 1159.5169
2365 1160.5535 1159.5462
2366 1160.5558 1159.5485
2367 1160.5562 1159.5489
2370 1160.5692 1159.5619
2371 580.8254 1159.6362
2372 1161.5510 1160.5437
2373 1161.5543 1160.5470
2374 1161.5809 1160.5736
2375 581.3313 1160.6480
2377 581.8034 1161.5922
2378 581.8160 1161.6174
2379 581.8169 1161.6192
2380 1163.5386 1162.5313
2381 1163.5587 1162.5514
2382 1163.5592 1162.5519
2383 1163.5616 1162.5543
2385 1164.5413 1163.5340
2386 1164.5459 1163.5386
2387 1164.5610 1163.5537
2388 582.8075 1163.6004
2389 1164.6106 1163.6033
2390 582.8110 1163.6074
2391 1164.6223 1163.6150
2392 1165.5465 1164.5392
2395 1165.5682 1164.5609
2396 583.7766 1165.5386
2398 1166.5472 1165.5399
2399 583.7828 1165.5510
2400 1166.5596 1165.5523
2401 1167.5669 1166.5596
2402 584.2962 1166.5778
2403 584.3112 1166.6078
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