MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2030 1080.5166 1079.5093
2031 1081.4919 1080.4846
2032 1081.5155 1080.5082
2033 541.2630 1080.5114
2034 1081.5197 1080.5124
2036 541.2645 1080.5144
2037 1081.5248 1080.5175
2038 1081.5299 1080.5226
2039 1082.5076 1081.5003
2040 1082.5149 1081.5076
2041 1082.5182 1081.5109
2042 1083.5149 1082.5076
2043 1083.5160 1082.5087
2045 1083.5322 1082.5249
2046 1084.5188 1083.5115
2048 1084.5281 1083.5208
2049 542.7682 1083.5218
2050 542.7738 1083.5330
2052 1085.5208 1084.5135
2053 543.2662 1084.5178
2054 1085.5271 1084.5198
2055 1085.5280 1084.5207
2056 543.3171 1084.6196
2057 1086.5187 1085.5114
2058 1087.5206 1086.5133
2059 1087.5311 1086.5238
2060 1087.5355 1086.5282
2061 544.2845 1086.5544
2065 544.2864 1086.5582
2066 544.2867 1086.5588
2068 1087.5677 1086.5604
2069 1087.5710 1086.5637
2070 1088.5131 1087.5058
2071 1088.5135 1087.5062
2073 544.7739 1087.5332
2075 1089.5037 1088.4964
2076 1089.5197 1088.5124
2077 1089.5314 1088.5241
2079 1090.5245 1089.5172
2081 1091.5082 1090.5009
2082 1091.5105 1090.5032
2083 1091.5197 1090.5124
2084 1091.5303 1090.5230
2085 546.2805 1090.5464
2086 1092.5132 1091.5059
2088 1092.5278 1091.5205
2090 1093.5225 1092.5152
2091 1093.5341 1092.5268
2092 1094.5159 1093.5086
2093 1094.5204 1093.5131
2094 1094.5209 1093.5136
2096 1094.5243 1093.5170
2097 1094.5323 1093.5250
2098 1094.5327 1093.5254
2100 1095.5092 1094.5019
2101 1095.5295 1094.5222
2102 548.7511 1095.4876
2103 1096.5131 1095.5058
2105 1096.5245 1095.5172
2107 549.0035 1095.9924
2108 1097.5294 1096.5221
2109 1097.5309 1096.5236
2110 1098.5183 1097.5110
2111 549.7640 1097.5134
2112 1098.5283 1097.5210
2113 1098.5305 1097.5232
2114 1098.5341 1097.5268
2115 1099.5066 1098.4993
2116 1099.5145 1098.5072
2117 1099.5273 1098.5200
2119 550.7526 1099.4906
2120 1100.5129 1099.5056
2121 1100.5227 1099.5154
2122 1100.5229 1099.5156
2123 1100.5347 1099.5274
2124 1100.5386 1099.5313
2125 550.7842 1099.5538
2126 1101.5216 1100.5143
2129 1102.5225 1101.5152
2130 1102.5270 1101.5197
2131 1102.5273 1101.5200
2132 1102.5312 1101.5239
2133 1102.5345 1101.5272
2136 1103.5239 1102.5166
2137 1103.5243 1102.5170
2138 1103.5244 1102.5171
2139 1103.5262 1102.5189
2140 552.2828 1102.5510
2142 1104.5155 1103.5082
2143 1104.5175 1103.5102
2144 1104.5220 1103.5147
2145 1104.5275 1103.5202
2146 1104.5334 1103.5261
2147 1105.5211 1104.5138
2148 1105.5305 1104.5232
2153 553.2925 1104.5704
2155 1106.5333 1105.5260
2156 1106.5339 1105.5266
2157 1107.5189 1106.5116
2158 1107.5300 1106.5227
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