MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1913 1050.5000 1049.4927
1914 1050.5020 1049.4947
1915 1050.5050 1049.4977
1916 1050.5126 1049.5053
1917 1050.5172 1049.5099
1918 1052.5040 1051.4967
1919 1052.5092 1051.5019
1920 1052.5112 1051.5039
1922 1053.5007 1052.4934
1923 527.2540 1052.4934
1924 1053.5021 1052.4948
1925 1053.5120 1052.5047
1926 1053.5133 1052.5060
1927 527.2910 1052.5674
1928 527.2914 1052.5682
1929 527.7441 1053.4736
1931 1054.5160 1053.5087
1932 1054.5197 1053.5124
1933 1054.5314 1053.5241
1934 1055.5092 1054.5019
1935 1055.5173 1054.5100
1936 1055.5276 1054.5203
1937 528.7391 1055.4636
1938 1056.4883 1055.4810
1939 1056.4933 1055.4860
1940 1056.4976 1055.4903
1941 1056.5034 1055.4961
1942 1056.5115 1055.5042
1945 1057.5040 1056.4967
1947 529.7510 1057.4874
1949 1058.5109 1057.5036
1950 1058.5116 1057.5043
1951 1058.5205 1057.5132
1952 1059.0110 1058.0037
1953 1059.5160 1058.5087
1954 1060.4847 1059.4774
1955 1060.4994 1059.4921
1956 1060.5089 1059.5016
1957 1061.5001 1060.4928
1958 1061.5034 1060.4961
1960 1061.5115 1060.5042
1961 1061.5145 1060.5072
1962 1061.5166 1060.5093
1963 531.2785 1060.5424
1965 1062.5078 1061.5005
1966 1062.5096 1061.5023
1967 1063.5005 1062.4932
1968 1063.5048 1062.4975
1970 1064.4934 1063.4861
1971 1064.4965 1063.4892
1972 1064.5029 1063.4956
1975 1064.5206 1063.5133
1976 532.7737 1063.5328
1977 1064.5415 1063.5342 1063.5410 -6.40 1     +0Score > 23 indicates identity
Score > 13 indicates homology		 RTAQFEANK
1978 1065.5062 1064.4989
1979 1065.5078 1064.5005
1980 1065.5106 1064.5033
1981 1065.5149 1064.5076
1982 1065.5254 1064.5181
1983 533.7624 1065.5102
1984 533.7637 1065.5128
1986 1067.5054 1066.4981
1987 1067.5062 1066.4989
1988 1067.5067 1066.4994
1989 1067.5182 1066.5109
1990 1067.5250 1066.5177
1991 1068.5010 1067.4937
1992 1068.5042 1067.4969
1993 1068.5155 1067.5082
1994 1068.5157 1067.5084
1996 1068.5277 1067.5204
1997 1069.5073 1068.5000
1998 1069.5181 1068.5108
1999 1070.5146 1069.5073
2000 1071.5055 1070.4982
2001 1071.5066 1070.4993
2002 1071.5120 1070.5047
2003 1072.5029 1071.4956
2004 1072.5065 1071.4992
2005 1072.5095 1071.5022
2006 1072.5156 1071.5083
2007 1072.5253 1071.5180
2008 536.7686 1071.5226
2009 1073.5059 1072.4986
2010 537.7487 1073.4828
2011 1074.4984 1073.4911
2012 1074.5106 1073.5033
2013 1075.5056 1074.4983
2014 1075.5173 1074.5100
2015 1075.5183 1074.5110
2016 1075.5193 1074.5120
2017 1076.0194 1075.0121
2018 1076.5061 1075.4988
2019 1076.5145 1075.5072
2020 1077.5197 1076.5124
2022 1077.5320 1076.5247
2023 1078.5045 1077.4972
2024 1078.5212 1077.5139
2026 1079.5198 1078.5125
2029 1080.5026 1079.4953
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