MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1796 1020.4866 1019.4793
1797 1020.4979 1019.4906
1798 1021.4824 1020.4751
1799 1021.4898 1020.4825
1800 511.2726 1020.5306
1802 1021.5436 1020.5363
1804 1022.4932 1021.4859
1805 1022.4932 1021.4859
1806 1022.5062 1021.4989
1808 1022.5298 1021.5225
1810 511.7728 1021.5310
1811 1023.4836 1022.4763
1812 512.2469 1022.4792
1814 512.2615 1022.5084
1815 512.2859 1022.5572
1816 1024.4796 1023.4723
1817 512.7440 1023.4734
1818 1024.4878 1023.4805
1819 1025.4801 1024.4728
1820 1025.4907 1024.4834
1821 513.2948 1024.5750
1822 1026.4858 1025.4785
1823 1026.4897 1025.4824
1824 1026.4943 1025.4870
1825 1026.4987 1025.4914
1826 1026.9862 1025.9789
1827 343.1311 1026.3715
1828 514.1932 1026.3718
1829 1027.4941 1026.4868
1830 1027.4951 1026.4878
1831 1028.5211 1027.5138
1832 1029.4984 1028.4911
1833 1029.5079 1028.5006
1834 1030.4862 1029.4789
1835 1030.4939 1029.4866
1836 1030.4980 1029.4907
1837 1030.4994 1029.4921
1838 1030.5002 1029.4929
1839 1030.5299 1029.5226
1841 1031.4857 1030.4784
1842 1031.4904 1030.4831
1844 1032.4774 1031.4701
1845 1032.5021 1031.4948
1846 1032.5051 1031.4978
1847 516.7661 1031.5176
1848 517.2394 1032.4642
1849 1033.4987 1032.4914
1850 1034.4910 1033.4837
1851 1034.4940 1033.4867
1852 1034.5007 1033.4934
1854 345.8071 1034.3995
1855 518.2071 1034.3996
1856 1035.4882 1034.4809
1857 1035.4900 1034.4827
1858 1035.5000 1034.4927
1859 1036.4927 1035.4854
1860 1036.5072 1035.4999
1862 1037.4905 1036.4832
1863 1037.4930 1036.4857
1864 1037.5168 1036.5095
1866 1038.4935 1037.4862
1867 1038.4991 1037.4918
1869 1039.4998 1038.4925
1870 520.2564 1038.4982
1871 1040.4879 1039.4806
1872 1040.4995 1039.4922
1874 1040.5092 1039.5019
1875 1041.4984 1040.4911
1876 1041.5088 1040.5015
1877 1042.5063 1041.4990
1880 521.7635 1041.5124
1881 1043.0271 1042.0198
1882 1043.4954 1042.4881
1883 1043.4999 1042.4926
1884 1043.5020 1042.4947
1885 1044.4973 1043.4900
1886 1044.5007 1043.4934
1887 1044.5056 1043.4983
1888 1044.5082 1043.5009
1889 522.7823 1043.5500
1890 1045.4999 1044.4926
1891 1045.5021 1044.4948
1892 1045.5031 1044.4958
1893 1045.5126 1044.5053
1894 1046.4974 1045.4901
1895 1046.5055 1045.4982
1897 1047.5107 1046.5034
1898 1047.5177 1046.5104
1899 524.2715 1046.5284
1900 1048.4722 1047.4649
1901 1048.5052 1047.4979
1903 524.7631 1047.5116
1904 524.7634 1047.5122
1905 524.7767 1047.5388
1906 524.7770 1047.5394
1907 1048.5530 1047.5457
1909 1049.5187 1048.5114
1910 525.2874 1048.5602
1911 1050.4893 1049.4820
1912 1050.4913 1049.4840
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